| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| Targets |
Galanin (porcine) targets galanin receptors GAL1, GAL2, and GAL3, which are G-protein coupled receptors. It acts as an agonist with pKi values of 9.63 at human GAL1, 9.49 at rat GAL1, 9.02 at human GAL2, 8.98 at rat GAL2, 8.01 at human GAL3, and 8.14 at rat GAL3. These receptors are expressed in the central and peripheral nervous systems, gastrointestinal tract, pancreas, adrenal gland, and urogenital tract.
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| ln Vitro |
In vitro, Galanin (porcine) binds to galanin receptor subtypes with high affinity and activates downstream signaling pathways through G-protein coupling. It influences neuronal excitability and synaptic plasticity in cultured neurons. The peptide inhibits the secretion of somatostatin, insulin, and glucose in various tissue preparations.
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| ln Vivo |
In vivo, Galanin (porcine) significantly increases food intake under free access conditions. It also plays roles in learning and memory, anxiety regulation, and sexual behavior. The peptide's effects are mediated through activation of galanin receptors in the central nervous system and peripheral tissues.
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| Enzyme Assay |
Receptor binding assays for Galanin (porcine) use membrane preparations from cells expressing human or rat GAL1, GAL2, or GAL3 receptors. Radiolabeled galanin is incubated with varying concentrations of the test peptide, and binding affinity (pKi) is calculated from competition binding curves.
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| Cell Assay |
Cellular assays for Galanin (porcine) typically use neuronal cell cultures or cells expressing galanin receptors. Cells are treated with the peptide, and downstream signaling pathways such as MAP kinase, calcium mobilization, or cAMP modulation are measured to assess receptor activation.
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| Animal Protocol |
In vivo studies with Galanin (porcine) are conducted in rodent models. The peptide is typically administered via intracerebroventricular (ICV) or intraperitoneal injection. Food intake, memory performance, anxiety-like behavior, and social behaviors are assessed using standardized behavioral paradigms.
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| ADME/Pharmacokinetics |
As a 29-amino acid peptide, Galanin (porcine) is expected to have a short half-life due to proteolytic degradation. The compound is soluble in water up to 0.50 mg/mL. It should be stored under appropriate conditions to maintain stability.
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| Toxicity/Toxicokinetics |
Toxicity data for Galanin (porcine) are limited. Peptide neuropeptides are generally well-tolerated at physiological concentrations. Standard laboratory safety practices should be followed when handling this research compound.
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| References | |
| Additional Infomation |
Galanin (porcine) is a research-use peptide and is not approved for therapeutic applications. It is also known as Porcine galanin. The peptide was first isolated from porcine small intestine and has been extensively studied for its role in various physiological processes. It is available from multiple research suppliers.
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| Molecular Formula |
C146H213N43O40
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|---|---|
| Molecular Weight |
3210.51751999999
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| Exact Mass |
3208.6
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| CAS # |
88813-36-9
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| Appearance |
White to off-white solid powder
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| LogP |
2.098
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~31.15 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (0.78 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (0.78 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (0.78 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.3115 mL | 1.5574 mL | 3.1148 mL | |
| 5 mM | 0.0623 mL | 0.3115 mL | 0.6230 mL | |
| 10 mM | 0.0311 mL | 0.1557 mL | 0.3115 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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