| Size | Price | Stock | Qty |
|---|---|---|---|
| 25g |
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| 50g |
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| Other Sizes |
Purity: ≥98%
| Targets |
Fuchsine base monohydrochloride targets cellular components including nucleic acids, proteins, and cell wall structures through electrostatic and hydrophobic interactions. In microbiology, it stains bacterial cell walls and is a key component in the Ziehl-Neelsen stain for acid-fast bacteria.
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|---|---|
| ln Vitro |
C.i. basic violet 14 is a dark green powder. A dye derived from triphenylmethane.
Rosanilin is a hydrochloride that is the monohydrochloride of 4-[(4-aminophenyl)(4-iminocyclohexa-2,5-dien-1-ylidene)methyl]-2-methylaniline. One of the major constituents of Basic fuchsin, together with pararosanilin, magenta II and new fuchsin. It has a role as a fluorochrome, a histological dye and a carcinogenic agent. It contains a rosanilin(1+). Fuchsine base monohydrochloride is a cationic dye that stains acidic cellular components. It is used as a primary stain in the Ziehl-Neelsen method for Mycobacterium tuberculosis and other acid-fast bacteria. It also stains nuclei, mitochondria, and other cellular structures in histological preparations. |
| ln Vivo |
As a histological stain, Fuchsine base monohydrochloride is not evaluated for in vivo therapeutic activity. Its application is in diagnostic pathology and microbiology for the visualization of microorganisms and tissue structures.
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| Enzyme Assay |
In vitro non-cell assays for Fuchsine base monohydrochloride are not enzyme-based. Its use involves staining fixed cells, tissues, or microorganisms on slides. Staining protocols include application of the dye solution, washing, differentiation (e.g., acid-alcohol for Ziehl-Neelsen), and counterstaining, followed by microscopic examination.
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| Cell Assay |
In vitro cell-based assays for Fuchsine base monohydrochloride involve staining cultured cells or microorganisms. Cells are fixed, stained with Fuchsine solution, and examined microscopically. The dye is also used in flow cytometry for DNA content analysis due to its binding to nucleic acids.
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| Animal Protocol |
In vivo animal studies are not performed with Fuchsine base monohydrochloride as it is a diagnostic stain rather than a therapeutic agent. It may be used ex vivo to stain tissue sections from animal studies for histopathological evaluation.
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| ADME/Pharmacokinetics |
Absorption, Distribution and Excretion
...Sixty mice were administered 12 mg of a magenta dye dissolved in peanut oil via gavage for 52 weeks (total dose 624 mg). Autopsy revealed that the dye had stained the tissues. Cationic dyes are known to bind to nucleic acids. /Cationic Dyes/ Cationic dyes appear to possess special properties of the nitrogen atom, which is part of the resonance system responsible for color, and appear to have a strong affinity for the carboxyl groups of corneal mucins. /Cationic Dyes/ Fuchsine base monohydrochloride has a molecular weight of 337.85 g/mol and formula C₂₀H₂₀ClN₃. It is soluble in water and alcohol, producing a magenta solution. As a dye, it does not exhibit systemic pharmacokinetics. It should be stored in a cool, dry place protected from light. |
| Toxicity/Toxicokinetics |
Fuchsine base monohydrochloride is considered a hazardous substance. It is a suspected carcinogen and should be handled with appropriate safety precautions, including the use of gloves and fume hoods. It can cause skin and eye irritation. Disposal should follow institutional guidelines for hazardous chemical waste.
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| References |
[1]. https://link.springer.com/article/10.1007/BF01324083
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| Additional Infomation |
Basic Violet 14 is a dark green powder, a dye derived from triphenylmethane. Rosanne is the hydrochloride of 4-[(4-aminophenyl)(4-iminocyclohexyl-2,5-diene-1-ethylene)methyl]-2-methylaniline. It is one of the main components of Basic Fuchsin, other main components of which include pararosanne, Fuchsin II, and neofuchsin. It is used as a fluorescent dye, histological dye, and carcinogen. It contains rosanne (1+) ions.
Fuchsine base monohydrochloride is widely used as a biological stain in microbiology (Ziehl-Neelsen stain for acid-fast bacteria, Gram stain) and histology. It is a key component of the Ziehl-Neelsen stain for the diagnosis of tuberculosis. Not approved for therapeutic use. Also known as Basic Violet 14 and Magenta. |
| Molecular Formula |
C20H20CLN3
|
|---|---|
| Molecular Weight |
337.8459
|
| Exact Mass |
337.134
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| CAS # |
632-99-5
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| Related CAS # |
3248-93-9 (Parent)
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| PubChem CID |
12447
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| Appearance |
METALLIC GREEN LUSTROUS CRYSTALS
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| Density |
0.999 g/mL at 20 °C
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| Boiling Point |
589.3ºC at 760 mmHg
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| Melting Point |
250 °C
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| Flash Point |
48 °F
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| Index of Refraction |
n20/D 1.334
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| LogP |
6.171
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
24
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| Complexity |
515
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| Defined Atom Stereocenter Count |
0
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| SMILES |
Cl[H].N([H])([H])C1C([H])=C([H])C(=C([H])C=1[H])C(C1C([H])=C([H])C(=C([H])C=1[H])N([H])[H])=C1C([H])=C([H])C(C(C([H])([H])[H])=C1[H])=N[H] |c:29,43|
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| InChi Key |
AXDJCCTWPBKUKL-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C20H19N3.ClH/c1-13-12-16(6-11-19(13)23)20(14-2-7-17(21)8-3-14)15-4-9-18(22)10-5-15;/h2-12,23H,21-22H2,1H3;1H
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| Chemical Name |
4-[(4-aminophenyl)-(4-imino-3-methylcyclohexa-2,5-dien-1-ylidene)methyl]aniline;hydrochloride
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| Synonyms |
Basic violet 14; 632-99-5; Fuchsin basic; Fuchsin; Basic fuchsine; MAGENTA; Fuchsine; Rosaniline;
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~147.99 mM)
H2O : < 0.1 mg/mL |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (6.16 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (6.16 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9599 mL | 14.7995 mL | 29.5989 mL | |
| 5 mM | 0.5920 mL | 2.9599 mL | 5.9198 mL | |
| 10 mM | 0.2960 mL | 1.4799 mL | 2.9599 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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