| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
|
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| 100mg | |||
| 250mg | |||
| Other Sizes |
| Targets |
Multiple targets including oxidative stress pathways and bacterial cell components.
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|---|---|
| ln Vitro |
Fraxidin is a coumarin with antioxidant, anti-inflammatory, and antifungal properties. It exhibits antibacterial activity against Bacillus subtilis with an inhibition zone of 12 mm at a concentration of 20 µg/disk. The compound scavenges free radicals, protecting cells from oxidative stress and inflammation.
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| ln Vivo |
In vivo efficacy of fraxidin has not been extensively characterized in publicly available literature. As a natural compound with antioxidant and anti-inflammatory properties, it may have potential in models of oxidative stress and inflammation. Its antifungal and antibacterial activities suggest potential in infectious disease models.
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| Enzyme Assay |
Antioxidant activity is assessed using cell-free assays such as DPPH or ABTS radical scavenging assays. Antibacterial activity is evaluated using standard broth microdilution or agar diffusion methods to determine minimum inhibitory concentrations (MICs) against various bacterial strains.
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| Cell Assay |
Antioxidant activity is evaluated in cell cultures exposed to oxidative stress. Cells are treated with fraxidin, and the levels of reactive oxygen species (ROS) are measured using fluorescent probes. Anti-inflammatory activity is assessed by measuring the production of inflammatory cytokines in stimulated immune cells. Antibacterial activity is assessed in bacterial cultures.
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| Animal Protocol |
In vivo studies for fraxidin are limited. Potential studies could be conducted in animal models of inflammation, oxidative stress, or bacterial infection. The compound would be administered orally or intraperitoneally. Inflammatory markers, oxidative stress markers, and bacterial load would be measured.
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| ADME/Pharmacokinetics |
Fraxidin has a molecular formula of C11H10O5 and a molecular weight of 222.19 g/mol. It is a coumarin compound. The compound is stored desiccated at -20°C.
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| Toxicity/Toxicokinetics |
Specific toxicological data for fraxidin are not extensively published. As a natural product, it is generally considered to have a favorable safety profile. However, as a research compound, it should be handled with standard laboratory safety precautions.
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| References | |
| Additional Infomation |
Fraxidin is a hydroxycoumarin. It has been reported to exist in Artemisia minor, Salsola laricifolia, and other organisms with relevant data.
Fraxidin is a research tool compound for studying the pharmacology of natural coumarins. It is not approved for clinical use. The compound is a natural product with antioxidant, anti-inflammatory, antifungal, and antibacterial activities. It is also known as a class of coumarin. |
| Molecular Formula |
C11H10O5
|
|---|---|
| Molecular Weight |
222.19
|
| Exact Mass |
222.052
|
| CAS # |
525-21-3
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| PubChem CID |
3083616
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| Appearance |
Off-white to light yellow solid powder
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
424.2±45.0 °C at 760 mmHg
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| Melting Point |
196-197ºC
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| Flash Point |
171.0±22.2 °C
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| Vapour Pressure |
0.0±1.0 mmHg at 25°C
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| Index of Refraction |
1.588
|
| Source |
Originated from plants: other families
|
| LogP |
0.56
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
5
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
16
|
| Complexity |
301
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
O=C1C=CC2=CC(OC)=C(OC)C(O)=C2O1
|
| InChi Key |
QNFBKOHHLAWWTC-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C11H10O5/c1-14-7-5-6-3-4-8(12)16-10(6)9(13)11(7)15-2/h3-5,13H,1-2H3
|
| Chemical Name |
8-hydroxy-6,7-dimethoxychromen-2-one
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| Synonyms |
EINECS 208-370-7; Fraxidin
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~100 mg/mL (~450.1 mM; with ultrasonication)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (11.25 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (11.25 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (11.25 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.5007 mL | 22.5033 mL | 45.0065 mL | |
| 5 mM | 0.9001 mL | 4.5007 mL | 9.0013 mL | |
| 10 mM | 0.4501 mL | 2.2503 mL | 4.5007 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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