| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Fmoc-Phe-OH-d5 does not have a classical biological receptor target; it is a protected amino acid used as a synthetic intermediate, and any biological activity is attributable to the phenylalanine-containing peptide product rather than the building block itself.
|
|---|---|
| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
In vitro, Fmoc-Phe-OH-d5 is used in solid-phase peptide synthesis and as an internal standard for MALDI-TOF-MS quantification of the μ-opioid receptor antagonist DAMGO in ovine plasma; the protected amino acid itself does not exhibit intrinsic receptor activity. |
| ln Vivo |
In vivo, Fmoc-Phe-OH-d5 is not administered as a therapeutic agent; it is used as a synthetic building block and analytical tracer, with any in vivo data relating to the biological effects of phenylalanine-containing peptides.
|
| Enzyme Assay |
Noncellular assays include HPLC or LC-MS/MS quantification of coupling efficiency during peptide synthesis and MALDI-TOF-MS quantification of DAMGO using Fmoc-Phe(D5)-OH as an internal standard after N-methylation.
|
| Cell Assay |
Cellular assays are not typically used for Fmoc-Phe-OH-d5 because it is a synthetic intermediate; biological activity is assessed using the synthesized phenylalanine-containing peptides rather than the protected amino acid.
|
| Animal Protocol |
Animal protocols are not used for Fmoc-Phe-OH-d5 because it is not a therapeutic candidate; any in vivo studies involve peptides synthesized with this building block rather than the protected amino acid itself.
|
| References |
| Molecular Formula |
C24H21NO4
|
|---|---|
| Molecular Weight |
387.42784
|
| Exact Mass |
392.178
|
| CAS # |
225918-67-2
|
| Related CAS # |
Fmoc-Phe-OH;35661-40-6
|
| PubChem CID |
76974603
|
| Appearance |
White to off-white solid powder
|
| LogP |
4.611
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
7
|
| Heavy Atom Count |
29
|
| Complexity |
551
|
| Defined Atom Stereocenter Count |
1
|
| SMILES |
[2H]C1=C(C(=C(C(=C1[2H])[2H])C[C@@H](C(=O)O)NC(=O)OCC2C3=CC=CC=C3C4=CC=CC=C24)[2H])[2H]
|
| InChi Key |
SJVFAHZPLIXNDH-XFTMQEPESA-N
|
| InChi Code |
InChI=1S/C24H21NO4/c26-23(27)22(14-16-8-2-1-3-9-16)25-24(28)29-15-21-19-12-6-4-10-17(19)18-11-5-7-13-20(18)21/h1-13,21-22H,14-15H2,(H,25,28)(H,26,27)/t22-/m0/s1/i1D,2D,3D,8D,9D
|
| Chemical Name |
(2S)-2-(9H-fluoren-9-ylmethoxycarbonylamino)-3-(2,3,4,5,6-pentadeuteriophenyl)propanoic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: ~100 mg/mL (~254.8 mM; with ultrasonication)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.37 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.5811 mL | 12.9056 mL | 25.8111 mL | |
| 5 mM | 0.5162 mL | 2.5811 mL | 5.1622 mL | |
| 10 mM | 0.2581 mL | 1.2906 mL | 2.5811 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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