| Size | Price | Stock | Qty |
|---|---|---|---|
| 250mg |
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| 500mg |
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| Other Sizes |
| ln Vivo |
- Fluorothyl is a potent seizure-inducing agent used to establish a rat model of recurrent neonatal seizure. Neonatal rats (Postnatal Day 7, P7) exposed to Fluorothyl exhibited recurrent generalized tonic-clonic seizures, which were scored using the Racine scale (Grade 3-4 seizures: forelimb clonus with rearing). Repeated exposure (once daily for 7 consecutive days) induced persistent neurodamage, including activation of apoptotic pathways (increased Bax/Bcl-2 ratio and cleaved caspase-3 expression), abnormal autophagy (elevated LC3-II/LC3-I ratio and Beclin-1 levels), and NF-κB-mediated inflammatory responses (upregulated p-NF-κB p65, IL-1β, and TNF-α expression in the hippocampus and cortex) [1]
- Fluorothyl-induced recurrent seizures led to significant neuronal loss in the hippocampal CA1 region and cerebral cortex (Nissl staining), as well as impaired neurobehavioral development (evaluated by negative geotaxis test and open field test) [1] |
|---|---|
| Animal Protocol |
- Rat model of recurrent neonatal seizure: Postnatal Day 7 (P7) Sprague-Dawley rats were randomly divided into control and Fluorothyl-treated groups. Fluorothyl was administered via inhalation in a sealed chamber (concentration adjusted to induce Grade 3-4 seizures within 1-3 minutes). Seizure onset time, duration, and severity were recorded using the Racine scale. Rats in the treatment group received Fluorothyl exposure once daily for 7 consecutive days (P7-P13), while the control group was exposed to vehicle in the same chamber. On P14, rats were sacrificed, and hippocampal and cortical tissues were collected for Western blot (apoptosis, autophagy, NF-κB pathway proteins), Nissl staining (neuronal count), and immunohistochemistry (inflammatory factor expression) [1]
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| Toxicity/Toxicokinetics |
Fluorothiazole exhibited significant neurotoxicity in newborn rats. Repeated induction of epilepsy led to apoptosis of hippocampal and cortical neurons, abnormal activation of autophagy, and exacerbation of neuroinflammation, resulting in neuronal loss and neurobehavioral deficits. [1]
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| References | |
| Additional Infomation |
Flurotyl is an ether compound. Flurotyl is a convulsant primarily used in laboratory animals. It has been used as an alternative to electroconvulsive therapy to induce seizures. Flurotyl is a synthetic small molecule compound widely used as a tool to induce seizures and to establish animal models of epilepsy[1]. Flurotyl's main function is to induce recurrent generalized seizures in newborn rats, mimicking clinical recurrent neonatal epilepsy, thus providing a model for studying neuroprotective strategies (e.g., IL-33-mediated neuroprotection)[1]. Flurotyl has no therapeutic use and is used only as a research reagent to induce pathological seizure states and associated neurological damage[1].
|
| Molecular Formula |
C4H4OF6
|
|---|---|
| Molecular Weight |
182.06436
|
| Exact Mass |
182.017
|
| CAS # |
333-36-8
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| PubChem CID |
9528
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| Appearance |
Colorless to light yellow liquid
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| Density |
1.404 g/mL at 25 °C(lit.)
|
| Boiling Point |
62-63 °C(lit.)
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| Melting Point |
122.5-123
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| Flash Point |
35 °F
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| Index of Refraction |
n20/D 1.300(lit.)
|
| LogP |
2.127
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
7
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
11
|
| Complexity |
98.7
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| Defined Atom Stereocenter Count |
0
|
| SMILES |
FC(F)(F)COCC(F)(F)F
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| InChi Key |
KGPPDNUWZNWPSI-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C4H4F6O/c5-3(6,7)1-11-2-4(8,9)10/h1-2H2
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| Chemical Name |
1,1,1-trifluoro-2-(2,2,2-trifluoroethoxy)ethane
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ≥ 125 mg/mL (~686.59 mM)
H2O : ~100 mg/mL (~549.27 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (11.42 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.4927 mL | 27.4635 mL | 54.9269 mL | |
| 5 mM | 1.0985 mL | 5.4927 mL | 10.9854 mL | |
| 10 mM | 0.5493 mL | 2.7463 mL | 5.4927 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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