| Size | Price | Stock | Qty |
|---|---|---|---|
| 5g |
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| Other Sizes |
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| ln Vitro |
The green fluorescent dye fluorescein has an acetylated derivative called fluorescein diacetate (FDA) [1]. Human Pi-like glutathione S-transferase (hGSTP1) uses fluorescein diacetate (FDA), a fluorescent probe, for intravital labeling in a variety of cytosolic GSTs. Fluorescent probes with selective activation. MCF7 cells exogenously overexpressing hGSTP1 showed fluorescent activation of fluorescein diacetate, which is susceptible to the GST inhibitor, but not hGSTA1 or hGSTM1 overexpressing cells. For hGSTP1-1, fluorescein diacetate can be employed as a fluorescent substrate. In order to determine whether hGSTP1 activity is the cause of fluorescence activation, recombinant hGSTP1-1 and GSH were treated with fluorescein diacetate in vitro. In the presence of both GSH and hGSTP1-1, there was a significant activation of the fluorescence; in the absence of either or when the enzyme was heat-inactivated, there was only a minor activation. This suggests that hGSTP1-1 activity is necessary for fluorescence activation by fluorescein diacetate. The specific enzyme activity of 1 μM fluorescein diacetate was found to be 79 ± 15 nmol/min/mg protein, based on the linear connection between the fluorescence increase rate and hGSTP1-1 concentration. One fluorescent substrate that can be employed for the in vitro evaluation of GSTP1-1 inhibitors is fluorescein diacetate. Both Ethacrynic Acid (EA) and NBDHEX suppressed the hGSTP1-1-dependent fluorescence increase in a concentration-dependent manner using fluorescein diacetate as the substrate, with IC50 values of 3.3±0.5 μM and 0.61±0.04 μM, respectively [2].
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|---|---|
| References |
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| Molecular Formula |
C24H16O7
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|---|---|
| Molecular Weight |
416.3796
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| Exact Mass |
416.089
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| CAS # |
596-09-8
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| PubChem CID |
65047
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.5±0.1 g/cm3
|
| Boiling Point |
604.7±55.0 °C at 760 mmHg
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| Melting Point |
200-203 °C(lit.)
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| Flash Point |
264.0±31.5 °C
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| Vapour Pressure |
0.0±1.7 mmHg at 25°C
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| Index of Refraction |
1.681
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| LogP |
2.77
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
31
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| Complexity |
708
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
CHADEQDQBURGHL-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C24H16O7/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24/h3-12H,1-2H3
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| Chemical Name |
(6'-acetyloxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl) acetate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~26.67 mg/mL (~64.05 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (5.00 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (5.00 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4017 mL | 12.0083 mL | 24.0165 mL | |
| 5 mM | 0.4803 mL | 2.4017 mL | 4.8033 mL | |
| 10 mM | 0.2402 mL | 1.2008 mL | 2.4017 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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