| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| Other Sizes |
| Targets |
Fluorescein-6-isothiocyanate does not have a specific biological target as a drug; it is a fluorescent dye. The isothiocyanate group reacts with primary amines on proteins, peptides, or other biomolecules to form a stable thiourea bond. This allows for the covalent attachment of the green fluorescent probe to the target molecule.
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|---|---|
| ln Vitro |
Fluorescein-6-isothiocyanate is not evaluated for biological activity as a drug; it is a fluorescent probe. Its utility is assessed by its fluorescence properties and reactivity with amines. It has Kd values of 8.74, 2.72, and 1.88 for N-Acetyl-L-Lysine, normal mouse IgG, and 4-4-20, respectively.
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| ln Vivo |
Fluorescein-6-isothiocyanate is not evaluated for in vivo biological activity as a drug; it is a fluorescent probe. It may be used in vivo for imaging applications, such as tracking labeled proteins or cells in animal models.
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| Enzyme Assay |
There are no specific in vitro enzyme or receptor binding assays for Fluorescein-6-isothiocyanate as a drug target. Its properties are characterized by absorbance and fluorescence spectra. The efficiency of labeling can be assessed by SDS-PAGE and fluorescence imaging.
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| Cell Assay |
There are no specific in vitro cellular assays for Fluorescein-6-isothiocyanate as a pharmacologically active compound. As a fluorescent probe, it is used to label proteins or other biomolecules. Cells are incubated with the labeled molecule, and fluorescence is visualized by microscopy or flow cytometry.
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| Animal Protocol |
There are no specific in vivo animal studies for Fluorescein-6-isothiocyanate as a pharmacologically active compound. As a fluorescent probe, it may be used for in vivo imaging studies, where the labeled molecule is administered to animals and its distribution is tracked by fluorescence imaging.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of Fluorescein-6-isothiocyanate are not applicable, as it is a fluorescent probe rather than a drug. The compound is not intended for administration as a therapeutic agent.
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| Toxicity/Toxicokinetics |
The toxicity profile of Fluorescein-6-isothiocyanate is not extensively reported. As a fluorescent probe, it is not intended for human therapeutic use. The compound should be handled with care in a laboratory setting.
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| References | |
| Additional Infomation |
Fluorescein 6-isothiocyanate is the 6-isomer of Fluorescein isothiocyanate.
Fluorescein-6-isothiocyanate (CAS 18861-78-4) is a fluorescent isomeric haptenic probe used for labeling proteins and other biomolecules. It has a molecular formula of C21H11NO5S and a molecular weight of 389.38. The compound is available for research purposes only. |
| Molecular Formula |
C21H11NO5S
|
|---|---|
| Molecular Weight |
389.3807
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| Exact Mass |
389.035
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| CAS # |
18861-78-4
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| PubChem CID |
2762652
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| Appearance |
Light yellow to brown solid powder
|
| Density |
1.5±0.1 g/cm3
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| Boiling Point |
719.1±60.0 °C at 760 mmHg
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| Flash Point |
388.7±32.9 °C
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| Vapour Pressure |
0.0±2.4 mmHg at 25°C
|
| Index of Refraction |
1.754
|
| LogP |
4
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
1
|
| Heavy Atom Count |
28
|
| Complexity |
668
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
C1=CC2=C(C(=C1)N=C=S)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O
|
| InChi Key |
GTQFZXYECNSNNC-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C21H11NO5S/c23-12-2-5-15-18(8-12)26-19-9-13(24)3-6-16(19)21(15)17-7-11(22-10-28)1-4-14(17)20(25)27-21/h1-9,23-24H
|
| Chemical Name |
3',6'-dihydroxy-5-isothiocyanatospiro[2-benzofuran-3,9'-xanthene]-1-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~250 mg/mL (~642.05 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (5.34 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (5.34 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (5.34 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.5682 mL | 12.8409 mL | 25.6819 mL | |
| 5 mM | 0.5136 mL | 2.5682 mL | 5.1364 mL | |
| 10 mM | 0.2568 mL | 1.2841 mL | 2.5682 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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