| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| Targets |
Fluorescamine does not have a specific pharmacological target. As an amine-reactive fluorescent probe, it is used for the detection and labeling of primary amines in biological samples. The compound reacts with primary amino groups in amino acids, peptides, and proteins to form stable and highly fluorescent derivatives. The reaction is highly specific for primary amines, and unreacted fluorescamine is rapidly hydrolyzed to a non-fluorescent product. This makes fluorescamine a valuable tool for fluorometric assays of amino acids, proteins, and proteolytic enzymes.
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| ln Vitro |
In vitro, fluorescamine is used for the detection and quantification of primary amines, amino acids, peptides, and proteins. The compound reacts with primary amino groups under mild conditions to form stable and highly fluorescent derivatives. The reaction is completed in milliseconds and can be used to detect amine-containing compounds. Unreacted fluorescamine is rapidly converted to a non-fluorescent product by reaction with water, making amine detection highly specific. The reaction products exhibit strong fluorescence with excitation/emission maxima of approximately 370/488 nm in acetone. Fluorescamine is also used to block newly generated amino termini in protein sequence analysis.
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| ln Vivo |
In vivo, fluorescamine is not typically used as a therapeutic agent. It is primarily a research tool for the detection and quantification of primary amines in biological samples. The compound's rapid reaction with primary amines and low background fluorescence make it valuable for various analytical applications.
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| Enzyme Assay |
For non-cellular assays, fluorescamine is used as a reagent for the fluorometric detection of primary amines, amino acids, peptides, and proteins. The compound is added to samples containing primary amines, and the resulting fluorescence is measured at excitation/emission maxima of approximately 370/488 nm in acetone. The fluorescence intensity is proportional to the concentration of primary amines in the sample. The assay is highly sensitive and specific due to the rapid hydrolysis of unreacted fluorescamine. The compound can also be used to block newly generated amino termini in protein sequence analysis.
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| Cell Assay |
For in vitro cell-based assays, fluorescamine can be used to detect and quantify primary amines in cell culture media, cell lysates, and other biological samples. The compound reacts with primary amino groups in proteins, peptides, and amino acids to form fluorescent derivatives. The fluorescence can be measured using a fluorometer or fluorescence plate reader at excitation/emission of approximately 370/488 nm. The assay can be used to measure protein concentrations, monitor protease activity, or detect the release of amino acids.
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| Animal Protocol |
For in vivo animal studies, fluorescamine is not typically used as a therapeutic agent. It is primarily a research tool for analytical applications.
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| ADME/Pharmacokinetics |
The pharmacokinetic properties of fluorescamine include a molecular weight of 278.26. The compound is soluble in appropriate organic solvents. It displays excitation/emission maxima of 370/488 nm in acetone. Storage should be appropriate for the compound's stability.
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| Toxicity/Toxicokinetics |
The toxicological profile of fluorescamine has not been fully characterized. As a research reagent, it is generally considered to have low toxicity at the concentrations used for analytical applications. The compound is not intended for human use.
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| Additional Infomation |
A non-fluorescent reagent for the detection of primary amines, peptides, and proteins. The reaction product exhibits strong fluorescence.
See also: fluorescein (note moved to). Fluorescamine is a non-fluorescent spiro compound that reacts with primary amines to form highly fluorescent derivatives. It is widely utilized in biochemistry for the detection and quantification of primary amines, amino acids, peptides, and proteins. The reaction occurs rapidly under mild conditions and is highly specific. Unreacted fluorescamine is rapidly hydrolyzed to a non-fluorescent product, ensuring low background fluorescence. The reaction products exhibit strong fluorescence with excitation/emission maxima of approximately 370/488 nm in acetone. The compound is also known as Fluram or Ro 20-7234. |
| Molecular Formula |
C17H10O4
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|---|---|
| Molecular Weight |
278.2589
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| Exact Mass |
278.057
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| CAS # |
38183-12-9
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| PubChem CID |
37927
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| Appearance |
White to light yellow solid powder
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
550.3±50.0 °C at 760 mmHg
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| Melting Point |
153-157 °C(lit.)
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| Flash Point |
248.5±30.2 °C
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| Vapour Pressure |
0.0±1.5 mmHg at 25°C
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| Index of Refraction |
1.687
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| LogP |
2.95
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
21
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| Complexity |
503
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
ZFKJVJIDPQDDFY-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C17H10O4/c18-15-13(11-6-2-1-3-7-11)10-20-17(15)14-9-5-4-8-12(14)16(19)21-17/h1-10H
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| Chemical Name |
4'-phenylspiro[2-benzofuran-3,2'-furan]-1,3'-dione
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 33 mg/mL (~118.59 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (8.98 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.98 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (8.98 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.5938 mL | 17.9688 mL | 35.9376 mL | |
| 5 mM | 0.7188 mL | 3.5938 mL | 7.1875 mL | |
| 10 mM | 0.3594 mL | 1.7969 mL | 3.5938 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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