| Size | Price | Stock | Qty |
|---|---|---|---|
| 5g |
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| Other Sizes |
| Targets |
Flavanone has inhibitory activity for human estrogen synthetase (aromatase). It is an inhibitor of the ERK/p38/NF-κB signaling pathway. Flavanone also exhibits antioxidant and free radical scavenging activity. These activities contribute to its potential health benefits, including the reduction of the risk of certain chronic diseases, cardiovascular diseases, and some cancers.
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|---|---|
| ln Vitro |
In vitro studies demonstrate that flavanone inhibits aromatase activity and the ERK/p38/NF-κB signaling pathway. It exhibits antioxidant and free radical scavenging activity. The compound's biological activities can be assessed using various cell-based assays, including aromatase inhibition assays, antioxidant assays (e.g., DPPH radical scavenging), and anti-inflammatory assays measuring cytokine production.
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| ln Vivo |
Flavanone is not typically used as a therapeutic agent but is studied for its potential health benefits. As a dietary flavonoid, it may contribute to the beneficial effects of flavonoid-rich foods. Monitoring of flavanone content can be useful in characterizing the authenticity of lemon juice, measuring the adulteration of citrus juices, and identifying the presence of orange juice in fruit drinks.
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| Enzyme Assay |
For non-cellular assays, flavanone's aromatase inhibition can be evaluated using human placental microsomes or recombinant CYP19A1 enzyme. The enzyme is incubated with [³H]-androstenedione and NADPH in the presence of varying concentrations of flavanone. The conversion to [³H]-estrone is measured by scintillation counting. Antioxidant activity is assessed using the DPPH radical scavenging assay, where the reduction of DPPH absorbance is measured spectrophotometrically.
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| Cell Assay |
In vitro cellular assays for flavanone involve treating cells with the compound and measuring its effects on signaling pathways and antioxidant status. The inhibition of ERK/p38/NF-κB signaling can be assessed by Western blotting for phosphorylated ERK, p38, and NF-κB. Antioxidant activity in cells can be measured using assays for reactive oxygen species (ROS) generation and glutathione levels.
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| Animal Protocol |
Flavanone is not typically used in animal experiments as a therapeutic agent. It is a dietary compound that may be studied for its health effects in animal models of disease. For example, flavanone-rich diets may be fed to rodents to assess their effects on cardiovascular disease, cancer, or inflammation. The compound's pharmacokinetics and metabolism are also studied in animals.
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| ADME/Pharmacokinetics |
Flavanone has a molecular weight of 224.26 and a molecular formula of C₁₅H₁₂O₂. It is a solid at room temperature with a melting point of 76-78°C. The compound is sparingly soluble in water but soluble in organic solvents such as ethanol, DMSO, and acetone. It is typically stored at room temperature in a dry, dark place. Purity is typically ≥98%.
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| Toxicity/Toxicokinetics |
Flavanone is generally recognized as safe (GRAS) as a dietary flavonoid. It has low toxicity and is well-tolerated at the levels found in foods. However, high doses may cause gastrointestinal disturbances. Standard laboratory safety precautions should be followed when handling the compound.
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| References | |
| Additional Infomation |
Flavanone are the simplest members of the flavanone class, consisting of flavane with an oxygen substituent at the 4-position. They are derived from the hydrides of flavane. Flavanone have been reported to be found in hops, sage, and other organisms with relevant data.
Flavanone is a naturally occurring flavonoid that serves as a key intermediate in flavonoid biosynthesis. It has inhibitory activity for aromatase and the ERK/p38/NF-κB signaling pathway. It exhibits antioxidant and free radical scavenging activity. Flavanone is used as a reference standard in analytical chemistry for the characterization of citrus products. It is available from various commercial suppliers. |
| Molecular Formula |
C15H12O2
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|---|---|
| Molecular Weight |
224.25
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| Exact Mass |
224.083
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| CAS # |
487-26-3
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| PubChem CID |
10251
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| Appearance |
Off-white to yellow solid powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
386.2±42.0 °C at 760 mmHg
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| Melting Point |
76-78 °C(lit.)
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| Flash Point |
181.9±21.4 °C
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| Vapour Pressure |
0.0±0.9 mmHg at 25°C
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| Index of Refraction |
1.603
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| LogP |
3.62
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
17
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| Complexity |
281
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
ZONYXWQDUYMKFB-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C15H12O2/c16-13-10-15(11-6-2-1-3-7-11)17-14-9-5-4-8-12(13)14/h1-9,15H,10H2
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| Chemical Name |
2-phenyl-2,3-dihydrochromen-4-one
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| Synonyms |
NSC50393; NSC 50393; Flavanone
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~445.93 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (11.15 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (11.15 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (11.15 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.4593 mL | 22.2965 mL | 44.5931 mL | |
| 5 mM | 0.8919 mL | 4.4593 mL | 8.9186 mL | |
| 10 mM | 0.4459 mL | 2.2297 mL | 4.4593 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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