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| Other Sizes |
| Targets |
Fast Red ITR is a diazonium salt that does not have a specific biological receptor target. Its primary use is as a chromogenic substrate for alkaline phosphatase in histochemical and immunohistochemical applications. When reacted with alkaline phosphatase, it forms a red precipitate at the site of enzyme activity.
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| ln Vitro |
In vitro, Fast Red ITR is used as a substrate for alkaline phosphatase in histochemistry and immunohistochemistry. It is used to detect the presence and localization of alkaline phosphatase activity in tissue sections or cells. It produces a red-colored precipitate that can be visualized under a microscope.
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| ln Vivo |
Fast Red ITR is not typically used in vivo. Its applications are limited to in vitro histochemical and immunohistochemical staining.
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| Enzyme Assay |
In vitro, tissue sections or cells are incubated with a solution containing Fast Red ITR and an alkaline phosphatase substrate. If alkaline phosphatase is present, it catalyzes the reaction, resulting in the formation of a red precipitate at the site of enzyme activity. The samples are then counterstained and examined under a microscope.
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| Cell Assay |
In cell-based assays, cells are fixed and incubated with Fast Red ITR in the presence of an alkaline phosphatase substrate. The formation of a red precipitate indicates the presence of alkaline phosphatase activity. This can be used to detect cells expressing alkaline phosphatase or to visualize specific antigens when using alkaline phosphatase-conjugated antibodies.
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| Animal Protocol |
Fast Red ITR is not used in in vivo animal experiments. Its use is restricted to in vitro applications.
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| ADME/Pharmacokinetics |
Fast Red ITR has a molecular weight of 258.34 and formula C11H18N2O3S. It is a white to off-white solid powder. It is soluble in organic solvents. Storage: Store in a cool, dry place.
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| Toxicity/Toxicokinetics |
Specific toxicity data are not extensively documented. As a diazonium salt, it may be irritating to the skin, eyes, and respiratory tract. The compound is for research use only. It should be handled with appropriate safety precautions.
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| References |
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| Additional Infomation |
Fast Red ITR is also known as Azoic Diazo Component 42 and N1,N1-Diethyl-4-methoxymetanilamide. It is a nonlinear optical material and is used in alkaline phosphatase histochemistry.
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| Molecular Formula |
C11H18N2O3S
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|---|---|
| Molecular Weight |
258.3372
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| Exact Mass |
258.103
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| CAS # |
97-35-8
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| PubChem CID |
66804
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| Appearance |
White to off-white solid powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
410.6±55.0 °C at 760 mmHg
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| Melting Point |
102-105°C
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| Flash Point |
202.1±31.5 °C
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| Vapour Pressure |
0.0±1.0 mmHg at 25°C
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| Index of Refraction |
1.547
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| LogP |
1.65
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
17
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| Complexity |
322
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O=S(N(CC)CC)(C1C=C(N)C(OC)=CC=1)=O
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| InChi Key |
WBGVVXSCGNGJFL-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C11H18N2O3S/c1-4-13(5-2)17(14,15)9-6-7-11(16-3)10(12)8-9/h6-8H,4-5,12H2,1-3H3
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| Chemical Name |
3-amino-N,N-diethyl-4-methoxybenzenesulfonamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~387.09 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (9.68 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.68 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (9.68 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.8709 mL | 19.3543 mL | 38.7087 mL | |
| 5 mM | 0.7742 mL | 3.8709 mL | 7.7417 mL | |
| 10 mM | 0.3871 mL | 1.9354 mL | 3.8709 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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