| Size | Price | Stock | Qty |
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| 5mg |
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| ln Vitro |
Fagomine (D-fagomine) is a metastable sugar that coagulates enterotoxins locally and selectively. Fagomine has the ability to specifically coagulate fibriated enterotoxins, such as Escherichia coli, and prevents them from adhering to the intestinal mucosa. This may be because lectins and binding sugars, such mannose, have structural similarities. The effects of a high-fat, high-sugar diet (HFHS) on intestinal E. coli and E. coli can be altered by fagomine [2].
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| ln Vivo |
Natural dietary subsaccharide gomovine (D-fagomine) regulates body weight, fasting blood glucose levels, and putrefactive Enterobacteriaceae in response to high-energy-density diets in the short term [3]. The percentage of animals fed a high-fat and high-sugar diet (HFHS) and Fagomine (D-fagomine) increased at a much slower rate than that of animals fed HFHS (20.9%) as compared to the standard group [2].
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| Animal Protocol |
Animals and Diets: 22-week-old male Sprague-Dawley rats were housed under controlled conditions (22°C ± 2°C, 60% humidity, 12-hour light/dark cycle) with free access to water and feed. Rats (n=15) were randomly assigned to one of three dietary groups (n=5 per group) for 5 weeks: a standard diet (STD), a high-fat high-sucrose diet (HFHS), or a HFHS diet supplemented with 0.065% D-Fagomine (HFHS+FG). Diets were pelletized in-house by lyophilization from frozen emulsions. Feed consumption was monitored daily. Body weight was measured at the beginning and end of the 5-week intervention. Fecal samples were collected before the intervention and at week 5, and stored at -80°C until analysis. At the end of the study, animals were killed in accordance with ethical guidelines.
Fecal Bacterial Analysis: Total DNA was extracted from fecal samples using a commercial stool DNA kit and quantified. Quantitative real-time PCR (qRT-PCR) was performed using a real-time PCR system with SYBR Green I dye. Specific primers were used to amplify DNA from total bacteria, Enterobacteriales, and the *E. coli* subgroup. Standard quantification curves were generated using DNA from an *E. coli* strain. Cycling conditions included an initial denaturation step, followed by 45 cycles of denaturation, annealing at primer-specific temperatures (65°C for total bacteria, 61°C for Enterobacteriales and *E. coli*), and extension. Melting curve analysis was performed to confirm specificity. The relative abundance of Enterobacteriales and *E. coli* was calculated as a percentage of total bacteria counts using the 2^(ΔCp) method based on Cp values. Blood Analysis: Blood glucose levels were measured using a blood glucose meter with the enzyme electrode method. Plasma insulin was measured using a rat/mouse insulin ELISA kit. Fecal Energy Content: Energy content in feces was determined by differential scanning calorimetry. [2] |
| References |
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| Additional Infomation |
Fagomine is a piperidine compound. It has been reported to be found in silkworms, wolfberries, and other organisms with relevant data.
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| Molecular Formula |
C6H13NO3
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|---|---|
| Molecular Weight |
147.174
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| Exact Mass |
147.089
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| CAS # |
53185-12-9
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| Related CAS # |
53185-12-9;53185-13-0 (HCl);1337470-51-5 (tartrate);
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| PubChem CID |
72259
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| Appearance |
White to khaki solid powder
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| Density |
1.3±0.1 g/cm3
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| Boiling Point |
315.4±42.0 °C at 760 mmHg
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| Melting Point |
186 - 188 °C
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| Flash Point |
170.5±18.5 °C
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| Vapour Pressure |
0.0±1.5 mmHg at 25°C
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| Index of Refraction |
1.535
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| LogP |
-1.63
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
10
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| Complexity |
109
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| Defined Atom Stereocenter Count |
3
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| SMILES |
C1CN[C@@H]([C@H]([C@@H]1O)O)CO
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| InChi Key |
YZNNBIPIQWYLDM-HSUXUTPPSA-N
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| InChi Code |
InChI=1S/C6H13NO3/c8-3-4-6(10)5(9)1-2-7-4/h4-10H,1-3H2/t4-,5-,6-/m1/s1
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| Chemical Name |
(2R,3R,4R)-2-(hydroxymethyl)piperidine-3,4-diol
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| Synonyms |
Fagomine D-Fagomine 1,2,5-Trideoxy-1,5-imino-D-arabino-hexitol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ≥ 36 mg/mL (~244.62 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (679.49 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.7949 mL | 33.9743 mL | 67.9486 mL | |
| 5 mM | 1.3590 mL | 6.7949 mL | 13.5897 mL | |
| 10 mM | 0.6795 mL | 3.3974 mL | 6.7949 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
| NCT Number | Recruitment | interventions | Conditions | Sponsor/Collaborators | Start Date | Phases |
| NCT01528631 | COMPLETED | Other: D-Fagomine Other: D-Fagomine |
Glycaemic Response Insulinemic Response |
Bioglane | 2012-02 | Not Applicable |
| NCT01811303 | COMPLETEDWITH RESULTS | Other: D-fagomine | Reduction of Post-prandial Glycaemic Response | Bioglane | 2012-09 | Not Applicable |
| NCT06490263 | RECRUITING | Drug: Experimental: Mulberry Twig Alkaloids Tablets 126 mg single dose in healthy subjects Drug: Mulberry Twig Alkaloids Tablets with single dose in healthy subjects Drug: Mulberry Twig Alkaloids Tablets with multiple dose in healthy subjects Drug: Mulberry Twig Alkaloids Tablets with multiple dose in overweight or obese subjects |
Healthy Overweight and Obesity |
Beijing Wehand-Bio Pharmaceutical Co., Ltd | 2024-05-13 | Phase 1 |
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