| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
|
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| Other Sizes |
| ln Vitro |
Ethyl Orsellinate (0-50 μg/ml; 48 hours) suppresses Hep-2 cells, MCF-7 breast cancer cells and 786-0 renal cancer cells, as well as B16-F10 murine melanoma and non-cancerous Vero cells (IC50s), respectively. =31.2, 70.3, 47.5, 64.8 and 28.1 µg/ml) [2].
In human tumor cell lines (A549 lung carcinoma, HT29 colorectal carcinoma, MCF-7 breast carcinoma, HeLa cervical carcinoma), Ethyl Orsellinate exhibited concentration-dependent antiproliferative activity. The IC50 values were 22.6 μM (HeLa), 25.3 μM (A549), 28.9 μM (MCF-7), and 31.7 μM (HT29) after 48-hour treatment, as determined by MTT assay [1] In mammalian cell lines (L929 mouse fibrosarcoma, KB human oral epidermoid carcinoma, HepG2 human hepatocellular carcinoma), Ethyl Orsellinate induced dose-dependent cytotoxicity. The IC50 values were 18.5 μM (L929), 24.2 μM (KB), and 30.1 μM (HepG2) following 72-hour incubation, with significant reduction in cell viability at concentrations ≥20 μM [2] Microscopic observation revealed that Ethyl Orsellinate (30 μM, 48 hours) caused morphological changes in HT29 and KB cells, including cell shrinkage, membrane blebbing, and formation of apoptotic bodies, suggesting apoptotic cell death [1][2] |
|---|---|
| Cell Assay |
Human tumor cells (A549, HT29, MCF-7, HeLa) were cultured in RPMI 1640 medium supplemented with fetal bovine serum. Cells were seeded in 96-well plates at a density of 5×10³ cells/well and incubated for 24 hours to adhere. Ethyl Orsellinate was dissolved in dimethyl sulfoxide (DMSO) and added at concentration gradients (1-100 μM). After 48 hours of treatment, MTT reagent was added, and the absorbance was measured at 570 nm to calculate cell viability and IC50 values [1]
L929, KB, and HepG2 cells were maintained in DMEM medium with serum. Cells were plated in 96-well plates (1×10⁴ cells/well) and allowed to grow for 24 hours. Ethyl Orsellinate (0.5-50 μM) was added, and the cells were incubated for 72 hours. After fixation with trichloroacetic acid, SRB dye was added, and unbound dye was washed away. The absorbance at 540 nm was measured to determine cell survival rate, and IC50 values were calculated via linear regression [2] For morphological analysis, HT29 and KB cells were seeded on coverslips, treated with Ethyl Orsellinate (30 μM) for 48 hours, and observed under an inverted light microscope to record cellular changes [2] |
| References | |
| Additional Infomation |
2,4-Dihydroxy-6-methylbenzoate ethyl ester is a 4-hydroxybenzoate. It has been reported to exist in Diospyros maritima, Sideritis marmorea, and other organisms with relevant data. Oselate ethyl ester is a natural oselate ester derivative, which is a class of compounds isolated from lichen or fungal metabolites [1][2]. This compound exhibits broad-spectrum in vitro antitumor and cytotoxic activity against various human and mammalian tumor cell lines, with relatively high activity against L929 and HeLa cells [1][2]. The cytotoxic effects of oselate ethyl ester are associated with the morphological characteristics of apoptosis, but its specific molecular mechanisms (e.g., target proteins, signaling pathways) have not been elucidated in the literature [1][2].
|
| Molecular Formula |
C10H12O4
|
|---|---|
| Molecular Weight |
196.1999
|
| Exact Mass |
196.073
|
| CAS # |
2524-37-0
|
| PubChem CID |
75653
|
| Appearance |
White to light yellow solid powder
|
| Density |
1.3±0.1 g/cm3
|
| Boiling Point |
349.5±22.0 °C at 760 mmHg
|
| Melting Point |
129-132 °C(lit.)
|
| Flash Point |
138.6±15.8 °C
|
| Vapour Pressure |
0.0±0.8 mmHg at 25°C
|
| Index of Refraction |
1.567
|
| LogP |
2.91
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
3
|
| Heavy Atom Count |
14
|
| Complexity |
204
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
UQSRXQMIXSZGLA-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C10H12O4/c1-3-14-10(13)9-6(2)4-7(11)5-8(9)12/h4-5,11-12H,3H2,1-2H3
|
| Chemical Name |
ethyl 2,4-dihydroxy-6-methylbenzoate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~509.68 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (12.74 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (12.74 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (12.74 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.0968 mL | 25.4842 mL | 50.9684 mL | |
| 5 mM | 1.0194 mL | 5.0968 mL | 10.1937 mL | |
| 10 mM | 0.5097 mL | 2.5484 mL | 5.0968 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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