| Size | Price | Stock | Qty |
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| 100mg |
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| 1g |
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| ln Vitro |
Hydrogen peroxide is scavenged by the non-flavonoid phenolic compound ethyl gallate. Following a 24- or 48-hour ethyl gallate treatment, HL-60 cells displayed morphological alterations that included the production of apoptotic bodies and a shrinking of the cell membrane. In line with these outcomes, ethyl gallate treatment caused time- and dose-dependent reductions in cell viability, suggesting that ethyl gallate is cytotoxic to HL-60 cells. The percentage of sub-G1 phase cells rose in response to ethyl gallate treatment in a concentration- and time-dependent manner. The percentage of cells in sub-G1 phase rose from 2.9% at baseline to 26.5% or 52.6% after treatment with 50 μM or 75 μM ethyl gallate for 24 or 48 hours, respectively. Research has revealed that ethyl gallate was used to treat HL-60 cells. Following a 24-hour treatment with 75 μM ethyl gallate, Bcl-2 expression decreased while Bax and truncated Bid (tBid) expression rose [1].
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| ln Vivo |
Rats given ethyl gallate equivalents of Nile tilapia leaf extract and rats fed ethyl gallate alone did not differ significantly in serum total protein, albumin, globulin, and glucose. Nonetheless, there were notable variations in total bilirubin levels between rats given 500 mg/kg body weight (ethyl gallate equivalent to 10 mg/kg, 0.34±0.01 mg/dL) and Nile tilapia (L.) leaf extract given 500 mg/kg body weight. received 10 mg/kg body weight (0.26 ± 0.01 mg/dL) of ethyl gallate. Between the groups given 10 and 20 mg/kg of ethyl gallate and 500 and 1000 mg/kg body weight of Nile tilapia leaf extract (26.52±1.23 and 30.05±1.38 U/L), there was a significant presence of ALT. The distinction. Twenty.50±0.94 and twenty-four.67±1.13 U/L)[2].
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| References |
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| Additional Infomation |
Ethyl gallate is a gallate ester obtained by the formal condensation of gallic acid with ethanol. It has a role as a plant metabolite.
Ethyl gallate has been reported in Phyllanthus sellowianus, Acer truncatum, and other organisms with data available. |
| Molecular Formula |
C9H10O5
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|---|---|
| Molecular Weight |
198.1727
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| Exact Mass |
198.052
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| CAS # |
831-61-8
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| PubChem CID |
13250
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| Appearance |
White to off-white solid powder
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
447.3±40.0 °C at 760 mmHg
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| Melting Point |
149-153 °C
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| Flash Point |
185.0±20.8 °C
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| Vapour Pressure |
0.0±1.1 mmHg at 25°C
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| Index of Refraction |
1.611
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| LogP |
2.07
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
14
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| Complexity |
193
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
VFPFQHQNJCMNBZ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C9H10O5/c1-2-14-9(13)5-3-6(10)8(12)7(11)4-5/h3-4,10-12H,2H2,1H3
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| Chemical Name |
ethyl 3,4,5-trihydroxybenzoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 100 mg/mL (~504.62 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (12.62 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (12.62 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (12.62 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.0462 mL | 25.2309 mL | 50.4617 mL | |
| 5 mM | 1.0092 mL | 5.0462 mL | 10.0923 mL | |
| 10 mM | 0.5046 mL | 2.5231 mL | 5.0462 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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