| Size | Price | Stock | Qty |
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| 1g |
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| 10g |
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| 25g |
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| 50g | |||
| 100g | |||
| Other Sizes |
| Targets |
Ethopabate's primary target is folate metabolism. As a PABA antagonist, it inhibits the synthesis of folic acid, which is essential for the growth and development of protozoan parasites of the genus Eimeria. By disrupting the parasite's ability to synthesize this vital nutrient, Ethopabate prevents the establishment and progression of the infection.
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|---|---|
| ln Vitro |
In vitro, Ethopabate's activity is measured by its ability to inhibit the growth of Eimeria species in cell culture. It has intrinsic coccidiostatic potency, though it is often used in combination with other drugs like amprolium and nicarbazin to enhance its efficacy.
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| ln Vivo |
In vivo, Ethopabate is effective in preventing and controlling coccidiosis in poultry when administered as a feed additive. Its efficacy is often improved when used in combination with other coccidiostats.
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| Enzyme Assay |
The mode of action of Ethopabate is studied in cell-free assays that measure folate synthesis. In this system, the enzyme dihydropteroate synthase, which is involved in folate biosynthesis, is incubated with its substrates (e.g., PABA and pteridine) in the presence of Ethopabate. The inhibition of the enzyme's activity is measured.
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| Cell Assay |
For cellular assays, the antiprotozoal activity of Ethopabate is assessed in cultures of Eimeria parasites. The parasites are grown in host cells, and the compound's ability to inhibit their replication is determined.
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| Animal Protocol |
In vivo, Ethopabate is administered to poultry through their feed or drinking water. Its efficacy is evaluated in controlled trials by measuring the reduction in oocyst shedding, the severity of intestinal lesions, and the overall health and weight gain of the birds.
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| ADME/Pharmacokinetics |
Ethopabate has a molecular weight of 237.25 g/mol. As a feed additive, it is incorporated into poultry diets at specific concentrations to achieve therapeutic efficacy.
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| Toxicity/Toxicokinetics |
As a coccidiostat, Ethopabate has a low toxicity profile for the host (poultry) due to the selective toxicity of the folate biosynthesis pathway in the parasite.
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| References | |
| Additional Infomation |
Ethopabate acid is an aminobenzoic acid compound and a folic acid metabolism inhibitor used as an anticoccidial agent in poultry. See also: amprolium; Ethopabate acid (ingredient); amprolium; bacitracin zinc; Ethopabate acid (ingredient); amprolium; babemycin; Ethopabate acid (ingredient)...View more...
Ethopabate is a veterinary drug, not intended for human use. It is used globally as a feed additive in the poultry industry to control coccidiosis, a common and economically significant disease. Its use is often part of a comprehensive program that includes management practices and vaccination. |
| Molecular Formula |
C12H15NO4
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|---|---|
| Molecular Weight |
237.2518
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| Exact Mass |
237.1
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| CAS # |
59-06-3
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| PubChem CID |
6034
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| Appearance |
White to off-white solid powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
426.1±35.0 °C at 760 mmHg
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| Melting Point |
148-151 °C
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| Flash Point |
211.5±25.9 °C
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| Vapour Pressure |
0.0±1.0 mmHg at 25°C
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| Index of Refraction |
1.546
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| LogP |
2.42
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
17
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| Complexity |
280
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O(C([H])([H])C([H])([H])[H])C1C([H])=C(C([H])=C([H])C=1C(=O)OC([H])([H])[H])N([H])C(C([H])([H])[H])=O
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| InChi Key |
GOVWOKSKFSBNGD-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C12H15NO4/c1-4-17-11-7-9(13-8(2)14)5-6-10(11)12(15)16-3/h5-7H,4H2,1-3H3,(H,13,14)
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| Chemical Name |
methyl 4-acetamido-2-ethoxybenzoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~210.75 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (10.54 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (10.54 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (10.54 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.2150 mL | 21.0748 mL | 42.1496 mL | |
| 5 mM | 0.8430 mL | 4.2150 mL | 8.4299 mL | |
| 10 mM | 0.4215 mL | 2.1075 mL | 4.2150 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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