| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
|
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| 500mg | |||
| 1g | |||
| Other Sizes |
| Targets |
Estrone sulfate potassium targets nuclear receptors in estrogen-responsive tissues. It is converted to estradiol, a more potent form of estrogen, through the activity of steroid sulfatase. This conversion is required for its biological activity.
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|---|---|
| ln Vitro |
In vitro, Estrone sulfate potassium promotes the colony formation of dispersed nitrosomethylurea mammary cells. Conversion to estradiol occurs both in vivo and in vitro. The compound is used to study human bone metabolism.
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| ln Vivo |
In vivo, Estrone sulfate potassium stimulates the growth of nitrosomethylurea-induced mammary tumors in ovariectomized rats. It is the most abundant estrogen in adult human circulation. The compound is used to study the role of sulfated estrogens in health and disease.
|
| Enzyme Assay |
The in vitro assay for Estrone sulfate potassium involves measuring its conversion to estradiol. Cells or tissue samples are incubated with the compound, and the production of estradiol is measured by immunoassay or mass spectrometry. Steroid sulfatase activity is assessed.
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| Cell Assay |
Cellular assays for Estrone sulfate potassium are performed using cultured osteoblasts and osteosarcoma cells as models for studying human bone metabolism. Cells are treated with the compound, and its effects on cell proliferation and differentiation are assessed.
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| Animal Protocol |
In vivo animal studies for Estrone sulfate potassium are conducted in ovariectomized rats. The compound is administered, and its effects on mammary tumor growth are measured.
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| ADME/Pharmacokinetics |
Estrone sulfate potassium is a natural endogenous steroid. It is the most abundant circulating estrogen. The compound is used as a research tool to study estrogen signaling and metabolism.
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| Toxicity/Toxicokinetics |
Toxicity data for Estrone sulfate potassium are not reported in the available sources. As a natural endogenous steroid, it is generally well-tolerated at physiological levels. Specific toxicological profiles are not provided.
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| References | |
| Additional Infomation |
Estrone sulfate potassium is a natural endogenous steroid and the most abundant estrogen in adult human circulation. It is converted to estradiol by steroid sulfatase. The compound is used in research on estrogen metabolism, bone biology, and hormone-dependent cancers. It is available from commercial suppliers for research purposes.
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| Molecular Formula |
C18H21O5S-.K+
|
|---|---|
| Molecular Weight |
388.51964
|
| Exact Mass |
389.083
|
| CAS # |
1240-04-6
|
| Related CAS # |
481-97-0 (Parent)
|
| PubChem CID |
23674502
|
| Appearance |
White to off-white solid powder
|
| LogP |
4.374
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
5
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
25
|
| Complexity |
630
|
| Defined Atom Stereocenter Count |
4
|
| SMILES |
C[C@]12CC[C@H]3[C@H]([C@@H]1CCC2=O)CCC4=C3C=CC(=C4)OS(=O)(=O)[O-].[K+]
|
| InChi Key |
CUQHOFAEIPGLBH-ZFINNJDLSA-M
|
| InChi Code |
InChI=1S/C18H22O5S.K/c1-18-9-8-14-13-5-3-12(23-24(20,21)22)10-11(13)2-4-15(14)16(18)6-7-17(18)19;/h3,5,10,14-16H,2,4,6-9H2,1H3,(H,20,21,22);/q;+1/p-1/t14-,15-,16+,18+;/m1./s1
|
| Chemical Name |
potassium;[(8R,9S,13S,14S)-13-methyl-17-oxo-7,8,9,11,12,14,15,16-octahydro-6H-cyclopenta[a]phenanthren-3-yl] sulfate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~257.39 mM)
H2O : < 0.1 mg/mL |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.43 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (6.43 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (6.43 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.5739 mL | 12.8694 mL | 25.7387 mL | |
| 5 mM | 0.5148 mL | 2.5739 mL | 5.1477 mL | |
| 10 mM | 0.2574 mL | 1.2869 mL | 2.5739 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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