Estriol (Oestriol)

Alias: NSC-12169; oestriol;NSC12169;NSC 12169;
Cat No.:V1753 Purity: ≥98%
Estriol (also called NSC12169;NSC 12169;NSC-12169; Oestriol) is a potent antagonist of the G-protein coupled estrogen receptor in estrogen receptor-negative breast cancer cells.
Estriol (Oestriol) Chemical Structure CAS No.: 50-27-1
Product category: Estrogenprogestogen Receptor
This product is for research use only, not for human use. We do not sell to patients.
Size Price Stock Qty
250mg
500mg
1g
2g
5g
10g
Other Sizes

Other Forms of Estriol (Oestriol):

  • Estriol-d3 (estriol d3)
  • Estriol-d2 (estriol d2)
  • Estriol-d (estriol d1)
  • Estriol-13C3 (Oestriol-13C3)
  • Estriol-d3-1
Official Supplier of:
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Purity & Quality Control Documentation

Purity: ≥98%

Product Description

Estriol (also called NSC12169; NSC 12169; NSC-12169; Oestriol) is a potent antagonist of the G-protein coupled estrogen receptor in estrogen receptor-negative breast cancer cells. Estriols has been successfully used in treating post-menopausal women. Its efficacy is due to the interference between estradiol-induced positive cooperative binding and receptor dimerization.

Biological Activity I Assay Protocols (From Reference)
ln Vitro

In vitro activity: MTT assays using G-1 shows that, in SkBr3 cells, the proliferative effect induced by 100 nM G-1 is abolished in the presence of 1 μM estriol which acts as an antagonist of GPR30-dependent pathway. A cell-free transcription assay demonstrates that the antiestrogenic activity exhibited by estriol is because of interferring with estradiol-induced positive cooperative binding and receptor dimerization, binding of hER complexes to ERE, as well as reducing estradiol-dependent transcription in a dose-dependent manner. A recent study shows that estrogen (estrone, estradiol, and estriol) inhibits Alzheimers disease-associated low-order Aβ oligomer formation, and among them, estriol shows the strongest in vitro activity.


Cell Assay: For the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, cells are cultured in plastic 96-well plates under 200 μL of growth medium and at an initial density of 10,000 cells per well. Cells are washed once they have attached and further incubated in medium containing 2.5% charcoal-stripped FBS with the indicated treatment. The medium is changed every 2 days (with treatment). Where applicable, 200 ng of the indicated plasmids are transfected every 2 days before treatments using Fugene6 Reagent, as recommended by the manufacturer. Following 6 days of incubation, the assay mixture (10µL per well) containing 1mg/mL 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) is added to each well and incubated at 37 ◦C for 4 h in a 5% CO2 atmosphere. The yellow tetrazolium MTT, reduced by metabolically active cells, results in intracellular purple formazan, which is released after overnight incubation with 200 µL 1% sodium dodecyl sulfate in 0.01N HCL and quantified spectrophotometrically by reading absorbance at 570nm using an enzyme-linked immunosorbent assay plate reader.

ln Vivo
In mPTEN+/- mice, estriol treatments resulted in a 187.54% gain in the relative ratio of uterine wet weight to body weight; estriol also increases the ratio to 176.88% in wild-type mice. Estriol treatment (20 mg/kg ip), in vivo, sensitizes Kupffer cells to LPS via mechanisms dependent on an increase in CD14 by elevated portal blood endotoxin caused by increased gut permeability in rats; while one-half of the rats given estriol intraperitoneally 24 hours before an injection of a sublethal dose of LPS (5 mg/kg) died within 24 hours.
Animal Protocol
Dissolved in ethanol and corn oil; 4 μg/g/day; s.c. administration
mPTEN+/- and wild-type mice
References
Mol Cell Endocrinol.2010 May 14;320(1-2):162-70;Lab Invest.2006 Mar;86(3):286-96.
These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C18H24O3
Molecular Weight
288.39
CAS #
50-27-1
SMILES
O([H])[C@@]1([H])[C@@]([H])(C([H])([H])[C@@]2([H])[C@]3([H])C([H])([H])C([H])([H])C4C([H])=C(C([H])=C([H])C=4[C@@]3([H])C([H])([H])C([H])([H])[C@@]21C([H])([H])[H])O[H])O[H]
Synonyms
NSC-12169; oestriol;NSC12169;NSC 12169;
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO:57 mg/mL (197.6 mM)
Water:<1 mg/mL
Ethanol:10 mg/mL (34.7 mM)
Solubility (In Vivo)
5%DMSO+95%Corn oil:10 mg/mL
 (Please use freshly prepared in vivo formulations for optimal results.)
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 3.4675 mL 17.3376 mL 34.6753 mL
5 mM 0.6935 mL 3.4675 mL 6.9351 mL
10 mM 0.3468 mL 1.7338 mL 3.4675 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

Calculator

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What volume of a given 10 mM stock solution is required to make 25 ml of a 25 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=25 μM, V2=25 ml and V1 is the unknown:
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g/mol

Molecular Weight Calculator allows you to calculate the molar mass and elemental composition of a compound, as detailed below:

Note: Chemical formula is case sensitive: C12H18N3O4  c12h18n3o4
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Definitions of molecular mass, molecular weight, molar mass and molar weight:
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In vivo Formulation Calculator (Clear solution)
Step 1: Enter information below (Recommended: An additional animal to make allowance for loss during the experiment)
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Calculation results

Working concentration mg/mL;

Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
             (2) Be sure to add the solvent(s) in order.

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