| Size | Price | |
|---|---|---|
| 50g | ||
| Other Sizes |
| Targets |
EDC-HCl does not have a biological target; it is a chemical crosslinking reagent. Its "targets" are carboxyl groups (-COOH) and primary amines (-NH₂) on biomolecules. EDC-HCl activates carboxyl groups to form an O-acylisourea intermediate, which reacts with primary amines to form stable amide bonds. This enables the covalent conjugation of proteins, peptides, and other molecules.
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|---|---|
| ln Vitro |
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC.HCl) is an extremely helpful chemical for forming amide bonds, or peptide bonds, in aqueous fluids [5]. In order to prepare fatty amines for HPLC and polyaniline-carbon nanotubes for cryogenic biosensors, 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC.HCl) is frequently utilized. The experiments include pre-column derivatization, solid-phase microregulation of peptides, preparation and detection of calcium sensors, creation of molecular beacons for DNA research, and simulation of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC.HCl) using fluorometric measurement [2]. Acid decarboxylase (GAD) does not significantly limit substrate degradation [3].
EDC-HCl itself is not a biologically active compound. Its utility lies in its chemical reactivity as a condensation and dehydrating agent. It accelerates the formation of esters, amides, and peptides. It is used in polynucleotide synthesis, hydroxylation, lactonization, and esterification. It enables the synthesis of peptides with specific sequences and functions. |
| ln Vivo |
EDC-HCl has no inherent in vivo biological activity as it is a chemical reagent. Its utility in vivo depends on the bioconjugates created using it. For example, EDC-HCl can be used to conjugate haptens to carrier proteins for immunization. The resulting conjugates can be administered to animals for antibody production. The crosslinker itself is not administered as a therapeutic agent.
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| Enzyme Assay |
There are no enzyme/receptor binding assays for EDC-HCl as it is not a drug targeting a biological receptor. However, the compound's reactivity with carboxyl and amine groups can be assessed by incubating it with carboxyl- and amine-containing molecules and analyzing conjugation efficiency using mass spectrometry, SDS-PAGE, or HPLC. The formation of amide bonds confirms successful conjugation.
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| Cell Assay |
Cellular assays for EDC-HCl involve labeling cell surface proteins or conjugating molecules to cells. Cells are treated with EDC-HCl and a molecule containing a primary amine. Conjugation efficiency is assessed by flow cytometry, fluorescence microscopy, or Western blotting using detection reagents specific to the conjugated molecule. Cell viability assays are performed to assess cytotoxicity.
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| Animal Protocol |
In vivo animal studies using EDC-HCl are typically conducted with bioconjugates prepared using EDC-HCl, such as hapten-carrier conjugates for immunization. The conjugate is administered to animals (e.g., mice) via injection. Antibody production is assessed by ELISA. The EDC-HCl crosslinker itself is not administered as a free compound in these studies.
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| ADME/Pharmacokinetics |
EDC-HCl has molecular weight of 191.7 g/mol and molecular formula C8H18ClN3. Melting point is approximately 113°C. It is a white to off-white crystalline powder. It is highly soluble in water. It is typically stored in a refrigerator. It is moisture-sensitive and should be stored under dry conditions.
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| Toxicity/Toxicokinetics |
EDC-HCl is a chemical reagent and is not intended for human therapeutic use. Standard laboratory safety precautions should be followed when handling EDC-HCl to avoid inhalation, ingestion, and skin contact. The compound is irritating to skin and eyes. It may cause allergic skin reactions. No systemic toxicity data is available as it is not intended for in vivo administration as a free compound.
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| References |
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| Additional Infomation |
EDC-HCl Crosslinker is a water-soluble, zero-length crosslinker. It activates carboxyl groups for reaction with primary amines to form amide bonds. It is used in peptide synthesis, protein conjugation, and hapten-carrier protein conjugation. It is a research reagent, not a therapeutic agent.
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| Molecular Formula |
C8H18CLN3
|
|---|---|
| Molecular Weight |
191.7016
|
| Exact Mass |
191.118
|
| CAS # |
25952-53-8
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| PubChem CID |
2723939
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| Appearance |
White to off-white solid powder
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| Density |
0.877 g/mL at 20 °C(lit.)
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| Boiling Point |
269.1ºC at 760 mmHg
|
| Melting Point |
110-115 °C(lit.)
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| Flash Point |
107.9ºC
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| Vapour Pressure |
0.171mmHg at 25°C
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| Index of Refraction |
n20/D 1.461
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| LogP |
1.933
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
5
|
| Heavy Atom Count |
12
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| Complexity |
134
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| Defined Atom Stereocenter Count |
0
|
| InChi Key |
FPQQSJJWHUJYPU-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C8H17N3.ClH/c1-4-9-8-10-6-5-7-11(2)3;/h4-7H2,1-3H3;1H
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~100 mg/mL (~521.65 mM)
DMSO : ~62.5 mg/mL (~326.03 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (13.04 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (13.04 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (13.04 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.2165 mL | 26.0824 mL | 52.1648 mL | |
| 5 mM | 1.0433 mL | 5.2165 mL | 10.4330 mL | |
| 10 mM | 0.5216 mL | 2.6082 mL | 5.2165 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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