| Size | Price | Stock | Qty |
|---|---|---|---|
| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
dtBHQ targets arachidonic acid (AA) metabolism by modulating the activities of 5-lipoxygenase (5-LO) and cyclooxygenase-2 (COX-2). These enzymes are key in the biosynthesis of leukotrienes and prostaglandins, respectively, which are potent inflammatory mediators. By inhibiting these enzymes, dtBHQ can influence inflammatory pathways. The compound's IC50 of 1.8 μM for 5-LO and 14.1 μM for COX-2 indicates a greater potency towards the lipoxygenase pathway.
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| ln Vitro |
Arachidonic acid (AA) metabolism is impacted by DTBHQ in vitro at physiologically meaningful doses [1].
In vitro, dtBHQ influences arachidonic acid (AA) metabolism in biologically relevant concentrations. It modulates 5-LO and COX-2 activities. As an indirect food additive and antioxidant, it can act as a stabilizer. Its effects on AA metabolism have been characterized in cell-free and cell-based systems. |
| ln Vivo |
In vivo, dtBHQ is primarily known as an indirect food additive and antioxidant. Its role in modulating arachidonic acid metabolism has been demonstrated in vitro. However, detailed in vivo efficacy data in animal models of inflammation or other diseases is limited in publicly available sources. It is also used as an antioxidant in rubber and plastics.
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| Enzyme Assay |
In vitro enzyme assays for dtBHQ measure its inhibition of 5-LO and COX-2 activities. For 5-LO, the enzyme is incubated with arachidonic acid, and the production of leukotrienes (e.g., LTB4) is measured by ELISA or HPLC. Various concentrations of dtBHQ are added to determine IC50 values. For COX-2, the enzyme is incubated with arachidonic acid, and prostaglandin E2 (PGE2) production is measured by ELISA. IC50 values are calculated from dose-response curves.
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| Cell Assay |
For in vitro cellular assays, cells such as macrophages or neutrophils are stimulated with calcium ionophore or LPS to induce AA metabolism. The cells are treated with various concentrations of dtBHQ. The production of leukotrienes and prostaglandins in the culture media is measured by ELISA or LC-MS. These assays confirm the compound's modulatory effects on AA metabolism.
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| Animal Protocol |
In vivo animal studies for dtBHQ are not typical, as it is primarily an industrial chemical and indirect food additive. However, its effects on AA metabolism could be studied in models of inflammation, though detailed protocols are not widely documented. It is used as an antioxidant in various industrial applications.
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| ADME/Pharmacokinetics |
dtBHQ has a molecular weight of 222.33 g/mol and a molecular formula of C14H22O2. It is a white to off-white solid powder with a melting point of 216-218°C. It is soluble in DMSO (~100 mg/mL). It is typically stored at room temperature. The compound is stable and used as an antioxidant.
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| Toxicity/Toxicokinetics |
dtBHQ is generally considered to have low toxicity and is used as an indirect food additive. It is also used as an antioxidant in rubber and plastics. However, standard laboratory safety precautions should be followed when handling the compound. It may cause skin and eye irritation. Comprehensive toxicity data is available from its use as a food additive and industrial chemical.
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| References | |
| Additional Infomation |
2,5-Di-tert-butylbenzene-1,4-diol is a hydroquinone compound with the molecular formula benzene-1,4-diol, in which tert-butyl groups are substituted at the 2 and 5 positions.
dtBHQ (2,5-Di-tert-butylhydroquinone) is an indirect food additive and a modulator of arachidonic acid metabolism. It inhibits 5-LO and COX-2 activities. It is also used as an antioxidant in rubber and plastics. It is available for research purposes only. |
| Molecular Formula |
C14H22O2
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|---|---|
| Molecular Weight |
222.33
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| Exact Mass |
222.161
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| CAS # |
88-58-4
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| PubChem CID |
2374
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| Appearance |
White to off-white solid powder
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| Density |
1.0±0.1 g/cm3
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| Boiling Point |
334.4±37.0 °C at 760 mmHg
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| Melting Point |
216-218 °C(lit.)
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| Flash Point |
151.5±21.1 °C
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| Vapour Pressure |
0.0±0.8 mmHg at 25°C
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| Index of Refraction |
1.520
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| LogP |
4.02
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
16
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| Complexity |
208
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
JZODKRWQWUWGCD-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C14H22O2/c1-13(2,3)9-7-12(16)10(8-11(9)15)14(4,5)6/h7-8,15-16H,1-6H3
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| Chemical Name |
2,5-ditert-butylbenzene-1,4-diol
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| Synonyms |
dt BHQ; dt-BHQ; 2,5-Di-tert-butylhydroquinone
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~449.80 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (11.25 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (11.25 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (11.25 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.4978 mL | 22.4891 mL | 44.9782 mL | |
| 5 mM | 0.8996 mL | 4.4978 mL | 8.9956 mL | |
| 10 mM | 0.4498 mL | 2.2489 mL | 4.4978 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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