| Size | Price | Stock | Qty |
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| 100mg |
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| 250mg |
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| 500mg | |||
| Other Sizes |
| Targets |
Dodecylphosphocholine is a detergent widely utilized in NMR studies of membrane proteins. It is known for its ability to solubilize membrane proteins while maintaining their native structure and functionality. It is also a substrate-type inhibitor of pancreas phospholipase A2.
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| ln Vitro |
By using NMR spectroscopy, laudecylphosphocholine micelles can be utilized to characterize the pore formation of the influenza M2 transmembrane (M2TM) domain [2].
Dodecylphosphocholine is extensively used in nuclear magnetic resonance (NMR) studies of membrane proteins. It forms micelles that mimic the lipid bilayer environment, allowing membrane proteins to be studied in a native-like state. The compound is also used as a model surfactant for studying micelle formation, protein–lipid interactions, and the transition between bilayer and micellar phases. |
| ln Vivo |
Dodecylphosphocholine is not used as a therapeutic agent in vivo. Its primary applications are in biochemical and biophysical research. The compound is used to solubilize membrane proteins for structural and functional studies. It is also used to study the effects of detergents on biological membranes and to develop new methods for membrane protein characterization.
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| Enzyme Assay |
In vitro biochemical assays for Dodecylphosphocholine involve studying its detergent properties. Critical micelle concentration (CMC) is determined by surface tension or fluorescence methods. The compound's ability to solubilize membrane proteins is assessed by measuring protein extraction efficiency and maintaining protein activity. NMR spectroscopy is used to study protein structure in DPC micelles. The compound's interaction with lipids and proteins is studied using biophysical techniques.
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| Cell Assay |
Cell-based assays for Dodecylphosphocholine are limited due to its detergent properties. The compound can be cytotoxic at high concentrations. When used in cell culture, it is typically employed at sub-lytic concentrations for membrane permeabilization or protein extraction. The compound's effects on cell viability and membrane integrity can be assessed using standard assays. Its use as a phospholipase A2 inhibitor can be studied in cell-based enzyme assays.
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| Animal Protocol |
In vivo animal studies for Dodecylphosphocholine are not common, as the compound is not a therapeutic agent. The compound is primarily used in research applications. Toxicological studies are limited. The compound is generally considered safe for laboratory use when handled appropriately. Its safety profile for in vivo applications has not been extensively characterized.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of Dodecylphosphocholine are not relevant for therapeutic applications. The compound is not intended for systemic administration. Its absorption, distribution, metabolism, and excretion have not been extensively studied. The compound is used in vitro, and its pharmacokinetics are not well-characterized.
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| Toxicity/Toxicokinetics |
Toxicological profile of Dodecylphosphocholine is characteristic of detergents. The compound can cause irritation upon contact with skin or eyes. Ingestion can cause gastrointestinal distress. Inhalation of aerosols can cause respiratory irritation. The compound is generally considered to have low toxicity at the concentrations used in research applications. Appropriate safety precautions should be taken.
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| References |
[1]. de Haas GH, Dijkman R, Ransac S, Verger R. Competitive inhibition of lipolytic enzymes. IV. Structural details of acylamino phospholipid analogues important for the potent inhibitory effects on pancreatic phospholipase A2. Biochim Biophys Acta. 1990;1046(3):249-257.
[2]. François Dehez, et al. Mitochondrial ADP/ATP Carrier in Dodecylphosphocholine Binds Cardiolipins with Non-native Affinity. Biophys J. 2017 Dec 5;113(11):2311-2315. [3]. Sarah D Cady, et al. Specific binding of adamantane drugs and direction of their polar amines in the pore of the influenza M2 transmembrane domain in lipid bilayers and dodecylphosphocholine micelles determined by NMR spectroscopy. J Am Chem Soc. 2011 Mar 30;133(12):4274-84. |
| Additional Infomation |
Dodecylphosphocholine is a monododecyl ester of phosphate choline, and it functions as a detergent.
Dodecylphosphocholine is a widely used detergent for membrane protein research, particularly in NMR studies. It is a valuable tool for studying membrane protein structure, dynamics, and function. The compound's zwitterionic nature makes it mild and suitable for maintaining protein native structure. It is also used as a model surfactant in biophysical studies of micelle formation and lipid-protein interactions. |
| Molecular Formula |
C17H38NO4P
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|---|---|
| Molecular Weight |
351.47
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| Exact Mass |
351.253
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| CAS # |
29557-51-5
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| Related CAS # |
Dodecylphosphocholine-d38;130890-78-7;Dodecylphosphocholine-d25;861924-55-2
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| PubChem CID |
644308
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| Appearance |
White to off-white solid powder
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| LogP |
1.46
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
16
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| Heavy Atom Count |
23
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| Complexity |
310
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CCCCCCCCCCCCOP(=O)([O-])OCC[N+](C)(C)C
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| InChi Key |
QBHFVMDLPTZDOI-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C17H38NO4P/c1-5-6-7-8-9-10-11-12-13-14-16-21-23(19,20)22-17-15-18(2,3)4/h5-17H2,1-4H3
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| Chemical Name |
dodecyl 2-(trimethylazaniumyl)ethyl phosphate
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| Synonyms |
DDPPC; DPC; Dodecylphosphocholine
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~100 mg/mL (~284.53 mM)
DMSO : ~32.5 mg/mL (~92.47 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (5.92 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (5.92 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (5.92 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.8452 mL | 14.2260 mL | 28.4519 mL | |
| 5 mM | 0.5690 mL | 2.8452 mL | 5.6904 mL | |
| 10 mM | 0.2845 mL | 1.4226 mL | 2.8452 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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