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| Other Sizes |
| Targets |
DMT-2'O-Methyl-rU Phosphoramidite does not have a direct biological target. It is a chemical reagent used in the synthesis of modified oligonucleotides. The 2'-O-methyl modification introduced by this phosphoramidite can enhance the stability and binding affinity of oligonucleotides. The compound is used in the preparation of oligonucleotides for research and therapeutic applications, including antisense and siRNA technologies. Its value is in nucleic acid chemistry.
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| ln Vitro |
DMT-2'O-Methyl-rU Phosphoramidite does not have direct in vitro biological activity. It is a chemical reagent used in oligonucleotide synthesis. Its in vitro activity would be evaluated indirectly through the biological activity of the modified oligonucleotides synthesized using it. The compound itself is not tested in biological assays but is used as a reagent in chemical synthesis.
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| ln Vivo |
DMT-2'O-Methyl-rU Phosphoramidite does not have in vivo biological activity. It is a chemical reagent used in oligonucleotide synthesis. The compound is not intended for administration to animals or humans. Its biological relevance is indirect, through the modified oligonucleotides that are synthesized using it.
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| Enzyme Assay |
In vitro enzyme/receptor binding assays are not applicable to DMT-2'O-Methyl-rU Phosphoramidite as it is a chemical reagent used in oligonucleotide synthesis, not a drug targeting a specific enzyme or receptor. The compound is characterized by chemical analysis methods such as HPLC for purity assessment (typically ≥95%), NMR for structural confirmation, and mass spectrometry. No enzyme or receptor binding studies have been reported.
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| Cell Assay |
Cell culture experiments are not applicable to DMT-2'O-Methyl-rU Phosphoramidite as it is a chemical reagent used in oligonucleotide synthesis, not a pharmacologically active compound. The compound is used in chemical synthesis and is not intended for cell-based assays. Researchers using this compound should focus on its chemical properties and synthetic applications.
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| Animal Protocol |
In vivo animal experiments are not applicable to DMT-2'O-Methyl-rU Phosphoramidite as it is a chemical reagent, not a therapeutic agent. The compound is not intended for administration to animals. Its biological relevance is indirect, through the modified oligonucleotides that are synthesized using it.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties are not applicable to DMT-2'O-Methyl-rU Phosphoramidite as it is a chemical reagent, not a therapeutic agent. The compound has a molecular formula of C40H49N4O9P and a molecular weight of 760.82. It is a solid. Storage: refrigerated (0-10°C), under inert gas.
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| Toxicity/Toxicokinetics |
Safety and toxicology data for DMT-2'O-Methyl-rU Phosphoramidite are limited. As a chemical reagent, it should be handled with appropriate safety precautions. The compound is for research use only and is not for human therapeutic use. Standard laboratory safety practices should be followed. No specific toxicity data are available.
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| References | |
| Additional Infomation |
DMT-2'O-Methyl-rU Phosphoramidite has CAS number 110764-79-9, molecular formula C40H49N4O9P, and molecular weight 760.82. It is a 2'-O-methyl modified ribonucleoside phosphoramidite used for oligonucleotide synthesis. Purity: ≥95%. Not for human use; for research purposes only.
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| Molecular Formula |
C40H49N4O9P
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|---|---|
| Molecular Weight |
760.8122
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| Exact Mass |
760.324
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| CAS # |
110764-79-9
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| PubChem CID |
10897989
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| Appearance |
White to light yellow solid powder
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| LogP |
6.136
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
11
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| Rotatable Bond Count |
18
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| Heavy Atom Count |
54
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| Complexity |
1240
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| Defined Atom Stereocenter Count |
4
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| SMILES |
CC(C)N(C(C)C)P(OCCC#N)O[C@@H]1[C@H](O[C@H]([C@@H]1OC)N2C=CC(=O)NC2=O)COC(C3=CC=CC=C3)(C4=CC=C(C=C4)OC)C5=CC=C(C=C5)OC
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| InChi Key |
UVUOJOLPNDCIHL-XKZJCBTISA-N
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| InChi Code |
InChI=1S/C40H49N4O9P/c1-27(2)44(28(3)4)54(51-25-11-23-41)53-36-34(52-38(37(36)49-7)43-24-22-35(45)42-39(43)46)26-50-40(29-12-9-8-10-13-29,30-14-18-32(47-5)19-15-30)31-16-20-33(48-6)21-17-31/h8-10,12-22,24,27-28,34,36-38H,11,25-26H2,1-7H3,(H,42,45,46)/t34-,36-,37-,38-,54?/m1/s1
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| Chemical Name |
3-[[(2R,3R,4R,5R)-2-[[bis(4-methoxyphenyl)-phenylmethoxy]methyl]-5-(2,4-dioxopyrimidin-1-yl)-4-methoxyoxolan-3-yl]oxy-[di(propan-2-yl)amino]phosphanyl]oxypropanenitrile
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~131.44 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.5 mg/mL (1.97 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 15.0 mg/mL clear DMSO stock solution to 400 μL of PEG300 and mix evenly; then add 50 μL of Tween-80 to the above solution and mix evenly; then add 450 μL of normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.5 mg/mL (1.97 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 15.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.3144 mL | 6.5719 mL | 13.1439 mL | |
| 5 mM | 0.2629 mL | 1.3144 mL | 2.6288 mL | |
| 10 mM | 0.1314 mL | 0.6572 mL | 1.3144 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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