| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg | |||
| 250mg | |||
| Other Sizes |
Purity: ≥98%
| Targets |
DiZPK is a photocrosslinker that functions as an aminoacyl-tRNA synthetase inhibitor. It is effective against fatty acid biosynthesis, protein synthesis, and cellular homeostasis. It binds to fatty acids in cells and prevents their demethylation.
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|---|---|
| ln Vitro |
In order to detect direct protein-protein interactions in living prokaryotic and eukaryotic cells, DiZPK, a structural analog of pyrrolysine (Pyl), functions as a photocross-linker [1].
In vitro, DiZPK is used to identify direct protein-protein interactions in living prokaryotic and eukaryotic cells. It is a photocrosslinking agent. It is a genetically encoded, highly efficient photocrosslinking amino acid. |
| ln Vivo |
In vivo, DiZPK can be used to identify direct protein-protein interactions in living prokaryotic and eukaryotic cells. It enables in vivo studies across microbiology, cell biology, and structural proteomics research.
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| Enzyme Assay |
In vitro assays for DiZPK typically involve incorporating the compound into proteins and using UV irradiation to induce crosslinking. Crosslinked protein complexes are identified by SDS-PAGE and Western blot or mass spectrometry.
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| Cell Assay |
Cell-based assays for DiZPK involve expressing proteins with the DiZPK amino acid in living cells, followed by UV irradiation to induce crosslinking. Crosslinked complexes are analyzed to identify protein-protein interactions.
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| Animal Protocol |
In vivo animal studies for DiZPK are limited. The compound is primarily used in cell culture systems. Its use in live animals has not been extensively explored.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of DiZPK are not well-characterized. The compound is typically used in cell culture media. Its absorption, distribution, metabolism, and excretion have not been studied.
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| Toxicity/Toxicokinetics |
The toxicological profile of DiZPK has not been extensively characterized. As a research compound, it should be handled with appropriate safety precautions.
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| References | |
| Additional Infomation |
DiZPK is a valuable research tool for studying protein-protein interactions in living cells. It is not approved for therapeutic use. The compound is used in microbiology, cell biology, and structural proteomics research.
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| Molecular Formula |
C12H23N5O3
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|---|---|
| Molecular Weight |
285.34
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| Exact Mass |
285.18
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| CAS # |
1337883-32-5
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| Related CAS # |
DiZPK hydrochloride;2349295-23-2
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| PubChem CID |
53314876
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| Appearance |
White to off-white solid powder
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| LogP |
1.183
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
10
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| Heavy Atom Count |
20
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| Complexity |
366
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| Defined Atom Stereocenter Count |
1
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| SMILES |
CC1(N=N1)CCCNC(=O)NCCCC[C@@H](C(=O)O)N
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| InChi Key |
YGUIFRMUMYTGAV-VIFPVBQESA-N
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| InChi Code |
InChI=1S/C12H23N5O3/c1-12(16-17-12)6-4-8-15-11(20)14-7-3-2-5-9(13)10(18)19/h9H,2-8,13H2,1H3,(H,18,19)(H2,14,15,20)/t9-/m0/s1
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| Chemical Name |
N6-((3-(3-Methyl-3H-diazirin-3-yl)propyl)carbamoyl)-L-lysine
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| Synonyms |
DiZPK CS-4117 EBP 883 BMS 790052
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~5.2 mg/mL (~18.22 mM)
DMSO : ~5 mg/mL (~17.52 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2 mg/mL (7.01 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.5046 mL | 17.5230 mL | 35.0459 mL | |
| 5 mM | 0.7009 mL | 3.5046 mL | 7.0092 mL | |
| 10 mM | 0.3505 mL | 1.7523 mL | 3.5046 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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