| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
DIPSO does not have a specific biological target. It is a zwitterionic buffer used to maintain pH in biochemical and biological experiments. Good's buffers are characterized by high water solubility, low cell membrane permeability, consistent acid-base dissociation constants, low metal chelating capability, and high chemical stability. DIPSO is used in various applications including biochemical and cell biology experiments. It can interfere with meiotic regulation in mouse oocytes.
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|---|---|
| ln Vitro |
DIPSO does not have direct in vitro biological activity as a pharmacological agent. Its value is as a buffer for biochemical and biological research. The compound maintains a stable pH environment for experiments without interfering with the biochemical processes being studied. It is used in buffer solutions for various in vitro applications including biochemical and cell biology experiments.
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| ln Vivo |
DIPSO does not have in vivo biological activity as a pharmacological agent. It is not intended for administration to animals or humans as a therapeutic. Its value is as a laboratory reagent for biochemical research. The compound is used in buffer solutions for various in vitro and ex vivo applications.
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| Enzyme Assay |
In vitro biochemical assays using DIPSO buffer are performed to maintain pH during experiments. The buffer is prepared at the desired pH within its effective range (7.0-8.2). DIPSO is used in biochemical and cell biology experiments. It has also been reported to interfere with meiotic regulation in mouse oocytes.
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| Cell Assay |
Cell culture experiments using DIPSO are possible as its pH range (7.0-8.2) is close to physiological pH. It can be used in cell culture media at appropriate concentrations. DIPSO may be used in specific applications where its buffering properties are advantageous.
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| Animal Protocol |
In vivo animal experiments are not applicable to DIPSO as it is a buffer reagent rather than a therapeutic agent. The compound is not intended for administration to animals. Its value is as a laboratory reagent for biochemical research.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties are not applicable to DIPSO as it is a buffer reagent rather than a therapeutic agent. The compound has a molecular weight of 243.28, a molecular formula of C7H17NO6S, and a pH range of 7.0-8.2. Solubility: H2O: 0.1 M at 20°C. Storage: room temperature.
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| Toxicity/Toxicokinetics |
DIPSO is considered a safe buffer for laboratory use with minimal toxicity at typical working concentrations. Good's buffers are designed for minimal interference with biochemical processes. However, as with all chemical reagents, appropriate safety precautions should be followed. The compound is for research use only and is not for human therapeutic use.
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| References |
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| Additional Infomation |
DIPSO has CAS number 68399-80-4, molecular formula C7H17NO6S, and molecular weight 243.28. Synonyms: 3-[bis(2-hydroxyethyl)amino]-2-hydroxypropane-1-sulfonic acid. It is a zwitterionic Good's buffer with a pH range of 7.0-8.2. Purity: typically ≥99%. Not for human use; for research purposes only.
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| Molecular Formula |
C7H17NO6S
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|---|---|
| Molecular Weight |
243.27798
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| Exact Mass |
243.077
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| CAS # |
68399-80-4
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| PubChem CID |
100210
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| Appearance |
White to off-white solid powder
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| Density |
1.5±0.1 g/cm3
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| Boiling Point |
449ºC
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| Melting Point |
189-192 °C(lit.)
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| Index of Refraction |
1.568
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| LogP |
-2.56
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
8
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| Heavy Atom Count |
15
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| Complexity |
243
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O=S(CC(O)CN(CCO)CCO)(O)=O
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| InChi Key |
XCBLFURAFHFFJF-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C7H17NO6S/c9-3-1-8(2-4-10)5-7(11)6-15(12,13)14/h7,9-11H,1-6H2,(H,12,13,14)
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| Chemical Name |
3-[bis(2-hydroxyethyl)amino]-2-hydroxypropane-1-sulfonic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~166.67 mg/mL (~685.10 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 50 mg/mL (205.52 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.1105 mL | 20.5524 mL | 41.1049 mL | |
| 5 mM | 0.8221 mL | 4.1105 mL | 8.2210 mL | |
| 10 mM | 0.4110 mL | 2.0552 mL | 4.1105 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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