| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
The primary target of Diphenyl-1-pyrenylphosphine is peroxides and hydroperoxides. It acts as a fluorogenic probe that reacts specifically with these reactive oxygen species. Upon oxidation, the probe is converted to the corresponding phosphine oxide, resulting in a change in its fluorescence properties. This allows for the detection and quantification of peroxides in various samples. The compound is also reported to target NF-κB.
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| ln Vitro |
In vitro, Diphenyl-1-pyrenylphosphine is used as a probe to detect peroxides in cell lysates, tissue homogenates, and other biological samples. The compound reacts with peroxides to form a fluorescent product, which can be measured using fluorescence spectroscopy or microscopy. It is used to study oxidative stress and the role of peroxides in various biological processes. The probe is also used in chemical analyses to detect peroxide contamination.
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| ln Vivo |
In vivo, Diphenyl-1-pyrenylphosphine may be used to detect peroxides in living organisms, although detailed in vivo applications are not extensively documented. The probe could be administered to animals to monitor oxidative stress in real-time. However, specific in vivo protocols and efficacy data are limited. The compound is primarily used as a research tool for in vitro detection of peroxides. Further studies are needed to evaluate its in vivo applications.
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| Enzyme Assay |
In vitro, Diphenyl-1-pyrenylphosphine is used as a fluorogenic probe for the detection of peroxides. The compound is dissolved in an appropriate solvent, such as DMSO or ethanol, and added to the sample. The reaction with peroxides is allowed to proceed at room temperature or 37°C for a specified time. The fluorescence intensity is measured using a fluorometer or fluorescence microscope, with excitation and emission wavelengths appropriate for the pyrene fluorophore. The protocol is used to quantify peroxide levels in biological and chemical samples.
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| Animal Protocol |
Specific in vivo animal experimental protocols for Diphenyl-1-pyrenylphosphine are not well-documented in the available literature. The compound is primarily used as a research tool for in vitro detection of peroxides. Researchers using this compound for in vivo studies would need to establish appropriate dosing and administration routes based on the specific research objectives. The compound is for research use only and not for human therapeutic use.
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| ADME/Pharmacokinetics |
Diphenyl-1-pyrenylphosphine has a molecular weight of 386.43 and the chemical formula C₂₈H₁₉P. It is a fluorogenic probe that reacts with peroxides. The compound is stable as a powder and should be stored at -20°C. It is soluble in organic solvents such as DMSO and ethanol. The compound is light yellow to yellow to green powder with a melting point of 174.0 to 178.0 °C.
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| Toxicity/Toxicokinetics |
Specific toxicity data for Diphenyl-1-pyrenylphosphine are not extensively documented. As a chemical probe, it may be irritating to the skin, eyes, and respiratory tract. The compound is for research use only and not for human therapeutic use. It should be handled with appropriate safety precautions, including the use of personal protective equipment. The compound should be stored in a sealed container in a cool, dry place away from incompatible materials.
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| References | |
| Additional Infomation |
Diphenyl-1-pyrenylphosphine is also known as DPPP. It is a fluorogenic peroxide-reactive probe used to detect peroxides and hydroperoxides. Upon reaction with peroxides, it is oxidized to the corresponding phosphine oxide, resulting in a change in fluorescence. It is used in biological and chemical research to study oxidative stress. It is supplied as a powder and should be stored at -20°C. It is for research use only.
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| Molecular Formula |
C28H19P
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|---|---|
| Molecular Weight |
386.4242
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| Exact Mass |
386.122
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| CAS # |
110231-30-6
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| PubChem CID |
130626
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| Appearance |
Off-white to light yellow solid powder
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| Boiling Point |
551.5±19.0 °C at 760 mmHg
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| Melting Point |
174.0 to 178.0 °C
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| Flash Point |
305.8±27.8 °C
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| Vapour Pressure |
0.0±1.4 mmHg at 25°C
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| LogP |
8.64
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
0
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
29
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| Complexity |
527
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
DSYGKYCYNVHCNQ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C28H19P/c1-3-10-23(11-4-1)29(24-12-5-2-6-13-24)26-19-17-22-15-14-20-8-7-9-21-16-18-25(26)28(22)27(20)21/h1-19H
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| Chemical Name |
diphenyl(pyren-1-yl)phosphane
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~12.5 mg/mL (~32.35 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (3.23 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.5879 mL | 12.9393 mL | 25.8786 mL | |
| 5 mM | 0.5176 mL | 2.5879 mL | 5.1757 mL | |
| 10 mM | 0.2588 mL | 1.2939 mL | 2.5879 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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