| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
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| 100mg |
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| Other Sizes |
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| Targets |
Coccidia
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|---|---|
| ln Vivo |
To enhance the anti-coccidial effect of dinitolmide and reduce its residual, the dinitolmide/MMT compounds were synthesized by the method of solution intercalation via dinitolmide intercalated into Na + -montmorillonite (Na + -MMT). The structure of compounds was characterized by X-ray diffraction and Fourier transformed infrared. Its anti-coccidial effect was examined by Eimeria tenella infection experiment. One hundred fifty AA broiler chickens were divided into five groups. Chickens were orally inoculated with 5 × 10(4) E. tenella oocysts after dinitolmide was given. Their curative effects were observed. The results showed that intercalated dinitolmide expanded the basal spacing (d 001) of MMT from 12.6 to 15.2 Å. The IR bands of amide group in dinitolmide/MMT were detected at 1,533 cm(-1) which showed that dinitolmide was successfully intercalated into the interlayer spaces of MMT. The dinitolmide/MMT showed higher anti-coccidian oocyst activity compared with dinitolmide (p < 0.05). The dinitolmide/MMT compound can significantly increase body weight gains and reduce bloody diarrhea, lesion score, and oocyst excretion. The anti-coccidia index of dinitolmide/MMT group (165.21) is much higher than dinitolmide group (88.84). The dinitolmide/MMT hybrid systems can be more effective in control of coccidiosis in comparison to dinitolmide[1].
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| Toxicity/Toxicokinetics |
Non-Human Toxicity Values
LD50 Rat oral 600 mg/kg LD50 Dog iv 75 mg/kg Ecotoxicity Values EC50 Daphnia magna (Water flea; intoxication, immobilization) 155 mg/L/48 hr (95% confidence interval: 146-164 mg/L); static PMID:938765 LC50 Daphnia magna (Water flea) 165 mg/L/48 hr (95% confidence interval: 157-173 mg/L); static Adverse Effects Occupational hepatotoxin - Secondary hepatotoxins: the potential for toxic effect in the occupational setting is based on cases of poisoning by human ingestion or animal experimentation. Methemoglobinemia - The presence of increased methemoglobin in the blood; the compound is classified as secondary toxic effect Non-Human Toxicity Values LD50 Rat oral 600 mg/kg LD50 Dog iv 75 mg/kg |
| References |
[1]. Effect of dinitolmide intercalated into Montmorillonite on E. tenella infection in chickens. Parasitol Res. 2014 Mar;113(3):1233-8.
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| Additional Infomation |
Yellowish, crystalline solid. Mp: 177 °C. Very slightly soluble in water. Soluble in acetone, acetonitrile, and dimethylformamide. Used as an anti-parasite drug for poultry. Store in a cool, ventilated place, away from acute fire hazards and easily oxidized materials.
Dinitolmide is a dinitrotoluene. Dinitolmide is a fodder additive for prophylaxis against coccidiosis infections in poultry. A coccidiostat for poultry. VET: /Dinitolmide exerts its/ greatest coccidiostatic activity against the asexual stages by arresting parasite development. Efficacy is limited to Eimeria tenell and E. necatrix unless combined with other products. FDA Food Additive Status Zoalene - FEED, REG, 2 ppm - Residues in uncooked fat of chickens - 556.770; 3 ppm - Residues in uncooked muscle meat of chickens - do; 3 ppm - Residues in uncooked muscle meat & liver of turkeys - do; 6 ppm - Residues in uncooked liver & kidneys of chickens - do; Use in chicken & turkey feeds - 558.60; 558.62; 558.78; 558.128; 558.248; 558.274; 558.450; 558.530; 558.680 Sources/Uses Used as a veterinary coccidiostat and food additive; [ACGIH] Coccidiostats are any of a group of chemical agents, e.g., nitrofurazone, nitrophenide, nicarbazin, mixed in feed or drinking water to control coccidiosis in animals. [Dorland's Illustrated Medical Dictionary] For 3,5-dinitro-o-toluamide (USEPA/OPP Pesticide Code: 037510) there are 0 labels match. /SRP: Not registered for current use in the U.S., but approved pesticide uses may change periodically and so federal, state and local authorities must be consulted for currently approved uses./ Therapeutic Uses (VET): Coccidiostat VET: /Dinitolmide exerts its/ greatest coccidiostatic activity against the asexual stages by arresting parasite development. Efficacy is limited to Eimeria tenell and E. necatrix unless combined with other products. |
| Molecular Formula |
C8H7N3O5
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|---|---|
| Molecular Weight |
225.1583
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| Exact Mass |
225.038
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| CAS # |
148-01-6
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| PubChem CID |
3092
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| Appearance |
Crystals from dilute alcohol
Yellowish, crystalline solid Yellowish solid or needles from aqueous ethanol |
| Density |
1.5±0.1 g/cm3
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| Boiling Point |
298.0±40.0 °C at 760 mmHg
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| Melting Point |
183-185 °C(lit.)
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| Flash Point |
134.0±27.3 °C
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| Vapour Pressure |
0.0±0.6 mmHg at 25°C
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| Index of Refraction |
1.636
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| LogP |
1.01
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
16
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| Complexity |
321
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O=C(C1C([H])=C(C([H])=C(C=1C([H])([H])[H])[N+](=O)[O-])[N+](=O)[O-])N([H])[H]
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| InChi Key |
ZEFNOZRLAWVAQF-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C8H7N3O5/c1-4-6(8(9)12)2-5(10(13)14)3-7(4)11(15)16/h2-3H,1H3,(H2,9,12)
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| Chemical Name |
2-methyl-3,5-dinitrobenzamide
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| Synonyms |
dinitolmide; 2-Methyl-3,5-dinitrobenzamide; 148-01-6; Zoalene; 3,5-Dinitro-o-toluamide; Zoamix; Coccidot; Coccidine A;
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 100 mg/mL (~444.13 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (11.10 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (11.10 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (11.10 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.4413 mL | 22.2064 mL | 44.4129 mL | |
| 5 mM | 0.8883 mL | 4.4413 mL | 8.8826 mL | |
| 10 mM | 0.4441 mL | 2.2206 mL | 4.4413 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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