| Size | Price | Stock | Qty |
|---|---|---|---|
| 5g |
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| 10g | |||
| 25g | |||
| Other Sizes |
| Targets |
Diflufenican inhibits phytoene desaturase (PDS) in the carotenoid synthesis pathway. By suppressing the production of PDS, it blocks carotenoid biosynthesis, inducing chlorophyll destruction and cell membrane rupture, ultimately causing the death of broadleaf weeds. It is a selective inhibitor of carotenoid biosynthesis.
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|---|---|
| ln Vitro |
In vitro, diflufenican inhibits phytoene desaturase gene expression and increases the production of phytoene in plant cell cultures. It inhibits phosphatase activity, an enzyme that hydrolyzes phosphate esters and releases free phosphate ions in cells. The compound's herbicidal activity has been characterized in various in vitro plant cell culture systems.
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| ln Vivo |
In vivo, diflufenican is effective against a range of broadleaf weeds in cereal crops such as wheat, barley, and rice. It provides both pre-emergence and early post-emergence control. The combination of diflufenican and flufenacet can be used to control Lolium rigidum populations resistant to ALS/ACCase. It has a long duration of activity and good crop safety.
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| Enzyme Assay |
In vitro enzyme assays for diflufenican typically measure its ability to inhibit phytoene desaturase activity. The enzyme is incubated with its substrate (phytoene) in the presence of increasing concentrations of the compound. The formation of downstream carotenoid products is measured by HPLC or spectrophotometry. IC50 values are calculated from dose-response curves.
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| Cell Assay |
Cell-based assays for diflufenican involve treating plant cell cultures (e.g., carrot cell cultures) with the compound and measuring carotenoid biosynthesis, phytoene accumulation, and gene expression. Cells are incubated with varying concentrations of the compound, and carotenoid levels are measured by HPLC. Phytoene desaturase gene expression is assessed by qRT-PCR.
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| Animal Protocol |
In vivo plant studies for diflufenican involve applying the compound to soil or foliage in greenhouse or field conditions. Weed control efficacy is assessed by measuring weed biomass, visual injury ratings, and crop safety. The compound's ability to inhibit weed growth and its selectivity for target crops are evaluated. Dose-response studies determine the optimal application rates.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of diflufenican are characteristic of systemic herbicides. The compound is absorbed by roots and foliage and translocated through the xylem. It accumulates in apical meristems and leaf tips. Metabolism occurs in plants. The compound has a molecular weight of 394.3 g/mol and a molecular formula of C19H11F5N2O2.
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| Toxicity/Toxicokinetics |
Diflufenican has low mammalian toxicity. It is an environmental pollutant but is considered safe for its intended agricultural use when applied according to label instructions. The compound is not a skin irritant and has low acute oral toxicity. Environmental persistence should be considered.
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| References | |
| Additional Infomation |
Diflufenican is a pyridine carboxamide with the structure pyridine-3-carboxamide, in which a 2,4-difluorophenyl atom is substituted at the carbamoyl nitrogen atom, and a 3-(trifluoromethyl)phenoxy atom is substituted at the 2-position. It is an environmental pollutant, exogenous substance, herbicide, and inhibitor of carotenoid biosynthesis. It is an aromatic ether, belonging to the (trifluoromethyl)benzene class of compounds and the pyridine carboxamide class.
Diflufenican is a selective herbicide used in agriculture for the control of broadleaf weeds. It is not approved for therapeutic use. The compound is a valuable tool for studying carotenoid biosynthesis and developing weed control strategies. It is a member of the pyridine carboxamide class of herbicides. |
| Molecular Formula |
C19H11F5N2O2
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|---|---|
| Molecular Weight |
394.301
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| Exact Mass |
394.074
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| CAS # |
83164-33-4
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| Related CAS # |
Diflufenican-d3;1185009-29-3
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| PubChem CID |
91735
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| Appearance |
Off-white to light yellow solid powder
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
376.2±42.0 °C at 760 mmHg
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| Melting Point |
110°C
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| Flash Point |
181.3±27.9 °C
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| Vapour Pressure |
0.0±0.9 mmHg at 25°C
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| Index of Refraction |
1.573
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| LogP |
3.65
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
28
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| Complexity |
533
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O=C(C1C(OC2C=C(C(F)(F)F)C=CC=2)=NC=CC=1)NC1C(F)=CC(F)=CC=1
|
| InChi Key |
WYEHFWKAOXOVJD-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C19H11F5N2O2/c20-12-6-7-16(15(21)10-12)26-17(27)14-5-2-8-25-18(14)28-13-4-1-3-11(9-13)19(22,23)24/h1-10H,(H,26,27)
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| Chemical Name |
N-(2,4-difluorophenyl)-2-[3-(trifluoromethyl)phenoxy]pyridine-3-carboxamide
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| Synonyms |
M&B 38544; Diflufenican
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~66.67 mg/mL (~169.09 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.34 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.5361 mL | 12.6807 mL | 25.3614 mL | |
| 5 mM | 0.5072 mL | 2.5361 mL | 5.0723 mL | |
| 10 mM | 0.2536 mL | 1.2681 mL | 2.5361 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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