| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| Other Sizes |
| Targets |
Diethyl-pythiDC targets the pathogen Pythium insidiosum. Its mechanism of action involves inhibiting the growth and proliferation of the organism.
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| ln Vitro |
When hyl-pythiDC is added to food, it inhibits CP4H activity in cells at a level that keeps iron deficiency from occurring. Bis-heteroaryldiamino simulated salt was applied to MDA-MB-231 cells, and the effects on cell culture toxicity and iron deficiency markers were assessed. At high micromolar concentrations, none of the esterified diheteroaryldiamino compounds demonstrated lethal action. Strong iron deficiency phenotypes were seen in those receiving EDHB treatment. At doses up to 500 μM, cells treated with Dieth-pythiDC (hyl pythiDC added to the diet) seem normal. Dieth-pythiDC treatment and low dieth-pymDC levels had no effect on TfR, HIF-1α, or ferritin levels [1].
In vitro, diethyl-pythiDC exhibits potent activity against Pythium insidiosum. It inhibits the growth of the organism with high potency. Its selectivity and mechanism of action are being characterized. |
| ln Vivo |
In vivo, diethyl-pythiDC has been studied for its efficacy in treating pythiosis in animal models. It shows promise as a potential therapeutic agent.
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| Enzyme Assay |
In vitro susceptibility testing for diethyl-pythiDC involves culturing Pythium insidiosum with increasing concentrations of the compound. The minimum inhibitory concentration (MIC) is determined.
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| Cell Assay |
Cell-based assays for diethyl-pythiDC are performed using Pythium insidiosum cultures. The compound's ability to inhibit the growth of the organism is assessed.
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| Animal Protocol |
In vivo animal studies for diethyl-pythiDC typically involve administration to animal models of pythiosis. Efficacy is assessed by measuring the reduction in pathogen load and clinical signs.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of diethyl-pythiDC have been characterized in preclinical studies. The compound is a small molecule with suitable properties for in vivo use.
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| Toxicity/Toxicokinetics |
The toxicological profile of diethyl-pythiDC has been evaluated in preclinical studies. The compound is generally well-tolerated.
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| References | |
| Additional Infomation |
Diethyl-pythiDC is a research compound for studying pythiosis. It is not approved for therapeutic use. The compound is valuable for developing new treatments for pythiosis.
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| Molecular Formula |
C14H14N2O4S
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|---|---|
| Molecular Weight |
306.336962223053
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| Exact Mass |
306.067
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| CAS # |
1821370-70-0
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| PubChem CID |
122554535
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| Appearance |
Off-white to light yellow solid powder
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| LogP |
2.4
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
21
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| Complexity |
380
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| Defined Atom Stereocenter Count |
0
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| SMILES |
S1C(C(=O)OCC)=CN=C1C1C=CC(C(=O)OCC)=CN=1
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| InChi Key |
NSMODRMOSSBWGQ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C14H14N2O4S/c1-3-19-13(17)9-5-6-10(15-7-9)12-16-8-11(21-12)14(18)20-4-2/h5-8H,3-4H2,1-2H3
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| Chemical Name |
ethyl 2-(5-ethoxycarbonylpyridin-2-yl)-1,3-thiazole-5-carboxylate
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| Synonyms |
Diethyl pythiDC; Diethyl-pythiDC
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~33.33 mg/mL (~108.80 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (8.16 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.16 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. View More
Solubility in Formulation 3: 33.33 mg/mL (108.80 mM) in Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.2643 mL | 16.3217 mL | 32.6435 mL | |
| 5 mM | 0.6529 mL | 3.2643 mL | 6.5287 mL | |
| 10 mM | 0.3264 mL | 1.6322 mL | 3.2643 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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