| Size | Price | Stock | Qty |
|---|---|---|---|
| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
Diethyl pyridine-2,4-dicarboxylate targets prolyl 4-hydroxylase, a key enzyme involved in the hydroxylation of proline residues during collagen biosynthesis. By inhibiting this enzyme, the compound interferes with procollagen processing.
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|---|---|
| ln Vitro |
In vitro, Diethyl pyridine-2,4-dicarboxylate is a potent prolyl 4-hydroxylase-directed proinhibitor. It inhibits prolyl hydroxylation and procollagen processing in chick-embryo calvaria. It is a pyridine derivative that has been extensively studied for its biological and pharmacological properties.
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| ln Vivo |
In vivo data for Diethyl pyridine-2,4-dicarboxylate are limited in the available literature. As a prolyl 4-hydroxylase inhibitor, it may have potential for research in collagen-related disorders. Further in vivo studies are needed to evaluate its therapeutic potential.
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| Enzyme Assay |
Diethyl pyridine-2,4-dicarboxylate's prolyl 4-hydroxylase inhibition activity can be characterized using biochemical assays. The compound's ability to inhibit prolyl hydroxylation can be assessed in chick-embryo calvaria or using purified enzyme preparations.
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| Cell Assay |
In vitro cell experiments with Diethyl pyridine-2,4-dicarboxylate involve treating cells with the compound and assessing its effects on collagen synthesis and processing. The compound's effects on prolyl hydroxylation and procollagen processing are evaluated.
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| Animal Protocol |
In vivo animal studies with Diethyl pyridine-2,4-dicarboxylate have been conducted using chick-embryo calvaria models. The compound's effects on bone development and collagen metabolism can be assessed. Further studies in mammalian models are needed.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for Diethyl pyridine-2,4-dicarboxylate are not available in the searched literature. As a small molecule with a molecular weight of 223.23, the compound may have reasonable bioavailability. Further pharmacokinetic studies are needed.
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| Toxicity/Toxicokinetics |
Diethyl pyridine-2,4-dicarboxylate is considered safe for research use at typical concentrations. As a research compound, it is intended for laboratory use only and not for human consumption. Standard laboratory safety practices should be followed.
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| References | |
| Additional Infomation |
Diethyl pyridine-2,4-dicarboxylate is a potent prolyl 4-hydroxylase-directed proinhibitor. It inhibits prolyl hydroxylation and procollagen processing in chick-embryo calvaria. It has a molecular formula of C₁₁H₁₃NO₄ and a molecular weight of 223.23.
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| Molecular Formula |
C11H13NO4
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|---|---|
| Molecular Weight |
223.2252
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| Exact Mass |
223.084
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| CAS # |
41438-38-4
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| PubChem CID |
170509
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| Appearance |
Off-white to light yellow solid powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
330.9±22.0 °C at 760 mmHg
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| Flash Point |
153.9±22.3 °C
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| Vapour Pressure |
0.0±0.7 mmHg at 25°C
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| Index of Refraction |
1.509
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| LogP |
2
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
16
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| Complexity |
254
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CCOC(=O)C1=CC(=NC=C1)C(=O)OCC
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| InChi Key |
MUUDQLHCIAOWPR-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C11H13NO4/c1-3-15-10(13)8-5-6-12-9(7-8)11(14)16-4-2/h5-7H,3-4H2,1-2H3
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| Chemical Name |
diethyl pyridine-2,4-dicarboxylate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ≥ 20 mg/mL (~89.6 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (11.20 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (11.20 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (11.20 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.4797 mL | 22.3984 mL | 44.7968 mL | |
| 5 mM | 0.8959 mL | 4.4797 mL | 8.9594 mL | |
| 10 mM | 0.4480 mL | 2.2398 mL | 4.4797 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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