| Size | Price | Stock | Qty |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg | |||
| Other Sizes |
| Targets |
DiD perchlorate integrates into cell membranes for labeling cells or membrane structures. It is suitable for long-term cell tracking and imaging. The dye is extensively used as a lipophilic tracer for labeling cells, organelles, liposomes, viruses, and lipoproteins.
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| ln Vitro |
First general protocol. As stock solutions, use dimethylimide (DMF) and dimethylimine (DMSO or 1–5 mM ethanol DMSO). DMF ethanol is the solvent. Stock remedies has to be used right away. Aliquot any excess solution, then freeze it at -20°C. The solution can be frozen and then thawed again for up to six months. 2) Prepare the working solution: To make a 1~5 μM working solution, add the storage solution to an appropriate buffer (serum, HBSS, or PBS). We advise against keeping the tank in storage for longer than a day. Note: The working solution's ultimate concentration ought to be determined by 2. 1) Centrifuge suspension cells at 1000 g for three to five minutes at 4°C; discard supernatant. Use PBS to wash twice, for five minutes each time. 1) Add 1 mL of Di working solution and stain for 5–30 minutes. The cell density is 1×106/mL. 3) Remove the supernatant after centrifuging at 400 g for three to four minutes at 4°C. 4) Use PBS to wash twice, for five minutes each time. 5) Re-suspend the cells in PBS or serum-free culture media. Utilize a flow cytometer or a fluorescence microscope to observe 3. Sterile coverslips are used to cultivate adherent cells. Second, add the spinal cord coverslip. Third, add 100 μL of working solution. Shake gently to cover all the cells. Fourth, work longitudinally for five to thirty minutes. 4) Wash for five minutes on medium two times. observation using flow cytometry or fluorescence microscopy.
DiD perchlorate is used as a fluorescent dye in vitro for labeling cell membranes and other hydrophobic structures. It is a long-chain carbocyanine dye that is widely used as a lipophilic tracer. Its fluorescence intensity is significantly enhanced when it binds to membranes or lipophilic biomolecules. |
| ln Vivo |
Following the injection of labeled cells, a bright DiD perchlorate (DiD) highlighted cell was discovered 15–40 μm from the intima a few days later. Consequently, the dispersion of the effects of DiD perchlorate labeling on cell division was consistent with the appearance of cell clusters with gradually decreasing staining intensity [3].
In vivo, DiD perchlorate is used for cell tracking and imaging in animal models. Its far-red/near-infrared fluorescence allows for deep tissue penetration and reduced background autofluorescence. It is suitable for long-term cell tracking studies, including labeling hematopoietic stem cells (HSCs). |
| Enzyme Assay |
In vitro assays for DiD perchlorate typically involve staining cells or membrane structures with the dye and visualizing them using fluorescence microscopy or flow cytometry. The dye's excitation and emission maxima are 644 nm and 665 nm, respectively, in the red/near-infrared spectrum.
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| Cell Assay |
Cell-based assays for DiD perchlorate involve incubating cells with the dye, which integrates into the cell membrane. Labeled cells can then be tracked over time or analyzed by flow cytometry. The dye is also used to label organelles, liposomes, viruses, and lipoproteins.
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| Animal Protocol |
In vivo animal studies for DiD perchlorate involve administering the dye to label cells or track their migration. The far-red/near-infrared fluorescence allows for non-invasive imaging in live animals. The dye is suitable for long-term tracking studies.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of DiD perchlorate are not relevant for therapeutic applications, as it is a fluorescent probe. The compound has a molecular weight of 959.9 g/mol and a molecular formula of C₆₁H₉₉ClN₂O₄. It is soluble in DMSO.
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| Toxicity/Toxicokinetics |
The toxicological profile of DiD perchlorate is characteristic of fluorescent dyes. It should be handled with appropriate safety precautions. Its use is limited to research applications.
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| References | |
| Additional Infomation |
DilC18(5) dye is a Cy5 dye and also an organic perchlorate. It is used as a fluorescent dye. It contains dilC18(5)(1+).
DiD perchlorate is a fluorescent probe used for labeling membranes and other hydrophobic structures. It is not approved for therapeutic use. The compound is a valuable tool for cell tracking, imaging, and flow cytometry. It is also known as a lipophilic red fluorescent probe. |
| Molecular Formula |
C61H99N2+.O4CL-
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|---|---|
| Molecular Weight |
959.902760000001
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| Exact Mass |
958.729
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| CAS # |
127274-91-3
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| PubChem CID |
16212738
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| Appearance |
Solid powder
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| LogP |
19.618
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
37
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| Heavy Atom Count |
68
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| Complexity |
1280
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CCCCCCCCCCCCCCCCCCN\1C2=CC=CC=C2C(/C1=C\C=C\C=C\C3=[N+](C4=CC=CC=C4C3(C)C)CCCCCCCCCCCCCCCCCC)(C)C.[O-]Cl(=O)(=O)=O
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| InChi Key |
ZQSBJPAQPRVNHU-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C61H99N2.ClHO4/c1-7-9-11-13-15-17-19-21-23-25-27-29-31-33-35-44-52-62-56-48-42-40-46-54(56)60(3,4)58(62)50-38-37-39-51-59-61(5,6)55-47-41-43-49-57(55)63(59)53-45-36-34-32-30-28-26-24-22-20-18-16-14-12-10-8-2;2-1(3,4)5/h37-43,46-51H,7-36,44-45,52-53H2,1-6H3;(H,2,3,4,5)/q+1;/p-1
|
| Chemical Name |
(2E)-2-[(2E,4E)-5-(3,3-dimethyl-1-octadecylindol-1-ium-2-yl)penta-2,4-dienylidene]-3,3-dimethyl-1-octadecylindole;perchlorate
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| Synonyms |
DiD perchlorate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. (3). This product is not stable in solution, please use freshly prepared working solution for optimal results. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~25 mg/mL (~26.04 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.67 mg/mL (1.74 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 16.7 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.67 mg/mL (1.74 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 16.7 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 1.67 mg/mL (1.74 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.0418 mL | 5.2089 mL | 10.4178 mL | |
| 5 mM | 0.2084 mL | 1.0418 mL | 2.0836 mL | |
| 10 mM | 0.1042 mL | 0.5209 mL | 1.0418 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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