| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 100mg |
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| 250mg | |||
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| Targets |
Dichotomitin is an isoflavonoid that targets pathways involved in inflammation and cell proliferation. Like other isoflavonoids, it is believed to have antioxidant, anti-inflammatory, and anticancer properties. Its mechanism of action is likely related to its ability to modulate signaling pathways such as NF-κB and to scavenge free radicals.
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| ln Vitro |
In vitro, Dichotomitin demonstrates biological activity consistent with its isoflavonoid structure. It exhibits antioxidant properties by scavenging free radicals. It also shows anti-inflammatory activity by inhibiting the production of inflammatory mediators. Its anticancer activity is assessed in cancer cell lines, where it inhibits cell proliferation and induces apoptosis.
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| ln Vivo |
In vivo, Dichotomitin is believed to contribute to the pharmacological effects of Belamcanda chinensis, which is used in traditional Chinese medicine. The plant is used for its anti-inflammatory and throat-soothing properties. Dichotomitin's antioxidant and anti-inflammatory activities may contribute to these effects. Specific standalone in vivo data are limited.
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| Enzyme Assay |
The in vitro antioxidant assay for Dichotomitin involves measuring its ability to scavenge free radicals, such as DPPH or ABTS. The compound is incubated with the radical, and the decrease in absorbance is measured. For anti-inflammatory activity, its ability to inhibit the production of nitric oxide or cytokines in LPS-stimulated macrophages can be measured.
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| Cell Assay |
In vitro cell culture studies for Dichotomitin utilize various cell lines. Its anticancer activity is assessed in cancer cell lines using MTT assays. Its anti-inflammatory effects can be studied in macrophage cell lines by measuring cytokine production. Its antioxidant effects can be studied by measuring its ability to reduce ROS levels in cells.
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| Animal Protocol |
In vivo animal experiments for Dichotomitin are not extensively documented. However, its anti-inflammatory and antioxidant effects could be studied in rodent models of inflammation or oxidative stress. The compound would be administered orally or intraperitoneally, and endpoints such as inflammatory cytokine levels, tissue damage, and oxidative stress markers would be assessed.
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| ADME/Pharmacokinetics |
Dichotomitin has a molecular formula of C18H14O8 and a molecular weight of 358.3 g/mol. It is a solid compound that is soluble in organic solvents like DMSO. It is typically stored as a powder at -20°C for long-term stability. Its stability is maintained under recommended storage conditions. As a natural product, it is often isolated from plant sources and is used as an analytical standard.
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| Toxicity/Toxicokinetics |
Toxicological data for Dichotomitin are limited, as it is a research compound. As a natural isoflavonoid, it is generally considered to have low toxicity. However, as a research compound, it is not intended for human therapeutic use, and comprehensive toxicity studies are not available. Standard safety precautions should be followed.
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| References | |
| Additional Infomation |
5,3'-Dihydroxy-4',5'-Dimethoxy-6,7-methylenedioxyisoflavones are isoflavone compounds. It has been reported that dihydroisoflavone glycosides are found in plants of the genus Iris potaninii, Iris domestica, and Iris germanica, and relevant data are available for reference.
Dichotomitin is a research compound with no clinical approval. It is a valuable tool for studying the pharmacology of isoflavonoids and their potential applications in health and disease. Its antioxidant, anti-inflammatory, and anticancer properties make it a compound of interest for research into chronic diseases, including cancer and inflammatory disorders. |
| Molecular Formula |
C18H14O8
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|---|---|
| Molecular Weight |
358.2990
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| Exact Mass |
358.068
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| CAS # |
88509-91-5
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| PubChem CID |
5316653
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| Appearance |
Off-white to light yellow solid powder
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| Density |
1.5±0.1 g/cm3
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| Boiling Point |
607.3±55.0 °C at 760 mmHg
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| Flash Point |
225.2±25.0 °C
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| Vapour Pressure |
0.0±1.8 mmHg at 25°C
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| Index of Refraction |
1.666
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| LogP |
2.79
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
26
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| Complexity |
578
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
PFFOGGCBLWTCPM-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C18H14O8/c1-22-12-4-8(3-10(19)17(12)23-2)9-6-24-11-5-13-18(26-7-25-13)16(21)14(11)15(9)20/h3-6,19,21H,7H2,1-2H3
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| Chemical Name |
9-hydroxy-7-(3-hydroxy-4,5-dimethoxyphenyl)-[1,3]dioxolo[4,5-g]chromen-8-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~11.11 mg/mL (~31.01 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.11 mg/mL (3.10 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 11.1 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.11 mg/mL (3.10 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 11.1 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 1.11 mg/mL (3.10 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7910 mL | 13.9548 mL | 27.9096 mL | |
| 5 mM | 0.5582 mL | 2.7910 mL | 5.5819 mL | |
| 10 mM | 0.2791 mL | 1.3955 mL | 2.7910 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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