| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Bacteria;Zika virus
Bacterial cell membranes (ionophore activity), viral entry mechanisms. Dianemycin acts as an ionophore, disrupting ion gradients across cell membranes. It is active against gram-positive bacteria, Mycobacterium, and drug-resistant strains of malaria. It also inhibits viral entry of Zika virus. |
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| ln Vitro |
Dianemycin(Nangangmycin) is used to treat chicken coccidiosis and as a growth promoter in poultry. Dianemycin is effective against malaria strains that are resistant to drugs[1].
A strong inhibitor of Zika virus (ZIKV) entry in all tested cell types, including physiologically relevant primary cells, is Dianemycin (Nanchangmycin). All three ZIKV strains are effectively inhibited from infecting any of the three cell types by dienicimycin. Dianemycin exhibits low toxicity within the range of 0.1 to 0.4 μM, which corresponds to the IC50s for infection. Furthermore, Dianemycin inhibits DENV in all cell types[2]. Dianemycin exhibits in vitro antibacterial properties against gram-positive bacteria. It is active against Mycobacterium, Botrytis cinerea, potato early blight, and Mycoplasma galli. It can inhibit coccidiosis infection and is active against drug-resistant strains of malaria. Dianemycin also exhibits antiviral properties against Zika virus by blocking viral entry. |
| ln Vivo |
Dianemycin can be used as a growth promotant in poultry and to cure coccidiosis in chickens. It is active against drug-resistant strains of malaria in vivo. The compound has been studied for its antimicrobial and antiparasitic activities in animal models.
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| Enzyme Assay |
In vitro antibacterial assays for Dianemycin involve determining minimum inhibitory concentrations (MICs) against a panel of gram-positive and gram-negative bacteria using broth dilution or agar diffusion methods. Antiviral activity against Zika virus is assessed in cell culture by measuring viral replication or entry inhibition. Antimalarial activity is evaluated using Plasmodium cultures.
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| Cell Assay |
The in vitro cellular activity of Dianemycin is evaluated in cell lines infected with Zika virus to assess its antiviral properties. Antibacterial activity is assessed in bacterial cultures. Antiparasitic activity is evaluated in Plasmodium cultures. The compound's ionophore activity can be assessed by measuring ion flux across membranes in various cell types.
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| Animal Protocol |
In vivo animal studies with Dianemycin typically involve poultry for evaluation as a growth promotant and for treatment of coccidiosis. Animal models of malaria are used to assess antimalarial activity. Dosing regimens and administration routes depend on the specific experimental objectives. The compound is typically administered in feed or by oral gavage.
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| ADME/Pharmacokinetics |
No specific pharmacokinetic data is available for Dianemycin. As a polyether ionophore (molecular weight 867.11, formula C47H78O14), it is expected to have poor oral bioavailability due to high molecular weight. The compound is typically used in veterinary applications and may have limited systemic absorption.
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| Toxicity/Toxicokinetics |
No specific toxicological data is available for Dianemycin. As an ionophore antibiotic, it may have toxicity concerns at high doses due to its effects on ion homeostasis. The compound has been used in veterinary applications, suggesting an acceptable safety profile at therapeutic doses. No clinical toxicity studies have been reported for human use.
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| References | |
| Additional Infomation |
Dianemycin (Nanchangmycin) is a polyether ionophore antibiotic produced by Streptomyces species. It has antibacterial, antiviral (Zika virus), and antiparasitic (malaria, coccidiosis) activities. It is used as a growth promotant in poultry and to treat coccidiosis in chickens. It is not approved for human clinical use.
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| Molecular Formula |
C47H78O14
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|---|---|
| Molecular Weight |
867.11
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| Exact Mass |
866.539
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| Elemental Analysis |
C, 65.10; H, 9.07; O, 25.83
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| CAS # |
35865-33-9
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| Related CAS # |
Nanchangmycin;65101-87-3
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| PubChem CID |
5475287
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| Appearance |
White to off-white solid powder
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| Density |
1.21g/cm3
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| Boiling Point |
910.3ºC at 760mmHg
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| Flash Point |
256.4ºC
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| Index of Refraction |
1.549
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| LogP |
6.149
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
14
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| Rotatable Bond Count |
13
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| Heavy Atom Count |
61
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| Complexity |
1570
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C[C@H]1[C@@]2(O[C@]([C@@]3([H])O[C@](O)([C@H](C)C[C@@H]3C)CO)([H])C[C@@H]2C)O[C@]([C@]4(O[C@]5(O[C@]([C@H](C)[C@@H](O)C5)([H])[C@@H](C)/C=C(C)/C([C@H](C)C[C@H](C)C(O)=O)=O)CC4)C)([H])C[C@@H]1O[C@@]6([H])O[C@@H]([C@@H](OC)CC6)C
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| InChi Key |
FELYAZAWTURXNF-JJIBRWJFSA-N
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| InChi Code |
InChI=1S/C47H78O14/c1-24(40(50)25(2)18-28(5)43(51)52)17-26(3)41-31(8)34(49)22-45(59-41)16-15-44(11,61-45)38-21-36(56-39-14-13-35(54-12)33(10)55-39)32(9)47(58-38)30(7)20-37(57-47)42-27(4)19-29(6)46(53,23-48)60-42/h17,25-39,41-42,48-49,53H,13-16,18-23H2,1-12H3,(H,51,52)/b24-17+
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| Chemical Name |
(E)-8-(9-hydroxy-2'-(6-hydroxy-6-(hydroxymethyl)-3,5-dimethyltetrahydro-2H-pyran-2-yl)-9'-((5-methoxy-6-methyltetrahydro-2H-pyran-2-yl)oxy)-2,4',8,10'-tetramethyl-1,1',6,6'-tetraoxa[2,7'-bispiro[4.5]decan]-7-yl)-2,4,6-trimethyl-5-oxonon-6-enoic acid
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| Synonyms |
Dianemycin; BRN1676784; BRN-1676784; BRN 1676784; Nanchangmycin
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~115.33 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.58 mg/mL (2.98 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.58 mg/mL (2.98 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.1533 mL | 5.7663 mL | 11.5326 mL | |
| 5 mM | 0.2307 mL | 1.1533 mL | 2.3065 mL | |
| 10 mM | 0.1153 mL | 0.5766 mL | 1.1533 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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