| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| ln Vitro |
In adult rat hippocampal slices, DHPG (100 μM) had no detectable effect on isoproterenol (Iso, 10 μM)-stimulated cAMP accumulation, in contrast to the robust potentiation observed with the mGluR agonist 1S,3R-ACPD. [1]
This lack of effect on cAMP potentiation, combined with its known activity on phosphoinositide hydrolysis, was used as key pharmacological evidence to argue that mGluR-mediated potentiation of cAMP responses is not secondary to phosphoinositide hydrolysis and is likely mediated by a group II-like mGluR. [1] In adult rat hippocampal slices, DHPG (100 μM) promotes the formation of [³H]-inositol phosphates ([³H]-IPs), although it produces only about half the maximal response of 100 μM (1S,3R)-ACPD.[2] When tested on phospholipase D (PLD) activity in hippocampal slices, DHPG (up to 1 mM) is virtually inactive as an agonist.[2] However, DHPG (100 and 300 μM) inhibits the formation of [³H]-phosphatidylethanol ([³H]-PEt) induced by 100 μM (1S,3R)-ACPD in a concentration-dependent manner.[2] |
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| References |
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| Additional Infomation |
2-Amino-2-(3,5-dihydroxyphenyl)acetic acid is an α-amino acid.
(RS)-3,5-dihydroxyphenylglycine (DHPG) is a selective pharmacological tool for activating type I metabologlutamate receptors (mGluR). According to transfected cell data cited in the literature, the EC50 value of DHPG for mGluR1 is approximately 60 μM. [1] Its pharmacological properties (DHPG is active for PI hydrolysis but inactive for cAMP enhancement in the detection system used) help to elucidate signaling pathways coupled with different mGluR subtypes. [1] DHPG is a recently discovered selective mGluR agonist belonging to group I mGluRs and coupled with phosphatidylinositol hydrolysis. [2] Studies have shown that DHPG can stimulate the phospholipase C (PLC) pathway but not the phospholipase D (PLD) pathway, and can even inhibit (1S,3R)-ACPD-induced PLD activity, suggesting that the coupling of mGluR with the PLC and PLD pathways is independent. [2] |
| Molecular Formula |
C8H9NO4
|
|---|---|
| Molecular Weight |
183.161362409592
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| Exact Mass |
183.053
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| CAS # |
146255-66-5
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| Related CAS # |
(S)-3,5-DHPG;162870-29-3
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| PubChem CID |
108001
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| Appearance |
Off-white to gray solid powder
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| Density |
1.55g/cm3
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| Boiling Point |
448.8ºC at 760mmHg
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| Flash Point |
225.2ºC
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| LogP |
0.882
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| Hydrogen Bond Donor Count |
4
|
| Hydrogen Bond Acceptor Count |
5
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
13
|
| Complexity |
187
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| Defined Atom Stereocenter Count |
0
|
| InChi Key |
HOOWCUZPEFNHDT-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C8H9NO4/c9-7(8(12)13)4-1-5(10)3-6(11)2-4/h1-3,7,10-11H,9H2,(H,12,13)
|
| Chemical Name |
2-amino-2-(3,5-dihydroxyphenyl)acetic acid
|
| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~5 mg/mL (~27.30 mM)
H2O : ~1 mg/mL (~5.46 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 0.5 mg/mL (2.73 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 5.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: 4.55 mg/mL (24.84 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication (<60°C). (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.4597 mL | 27.2985 mL | 54.5971 mL | |
| 5 mM | 1.0919 mL | 5.4597 mL | 10.9194 mL | |
| 10 mM | 0.5460 mL | 2.7299 mL | 5.4597 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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