Dexamethasone Sodium Phosphate

Alias: Dexamethasone sodium phosphate
Cat No.:V1837 Purity: ≥98%
Dexamethasone Sodium Phosphate, the salt form of Dexamethasoneadministeredthrough i.
Dexamethasone Sodium Phosphate Chemical Structure CAS No.: 55203-24-2
Product category: Autophagy
This product is for research use only, not for human use. We do not sell to patients.
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InvivoChem's Dexamethasone Sodium Phosphate has been cited by 2 publications
Purity & Quality Control Documentation

Purity: ≥98%

Product Description

Dexamethasone Sodium Phosphate, the salt form of Dexamethasone administered through i.v. injection, is a potent synthetic glucocorticoid class of steroid drugs, and an interleukin receptor modulator with anti-inflammatory and immunosuppressive activities. Dexamethasone has anti-inflammatory and immunosuppressant effects. It is 25-fold more potent than cortisol in its glucocorticoid effect, while having minimal mineralocorticoid effect. Dexamethasone is used for the treatment of many conditions including: rheumatologic problems, a number of skin diseases such as erythema multiforme, severe allergies, asthma.

Biological Activity I Assay Protocols (From Reference)
ln Vitro

In vitro activity: Dexamethasone inhibits COX-2 mRNA expression induced by IL-1 in human articular chondrocytes. Dexamethasone suppresses the cyclooxygenase-2 induction by tumor necrosis factor α (TNFα) with an IC50 of 1 nM in MC3T3-E1 cells. Dexamethasone binds to the glucocorticoid receptor and then to the glucocorticoid response element. Dexamethasone (10 μM) induces osteoblastic differentiation of rat bone marrow stromal cell cultures with elevated mRNA expression of alkaline phosphatase osteopontin, bone sialoprotein, and osteocalcin. Dexamethasone (5 μM) treatment decreases proliferation of adult hippocampal neural progenitor cells and SRE-driven gene expression.

ln Vivo
Dexamethasone (2 mg/kg) reduces the number of the BrdU-labeled hepatocytes by 80% in male Fischer F344 rats. Dexamethasone (2 mg/kg) pretreatment suppresses the expression of both TNF and IL-6 after partial hepatectomy and significantly reduces the proliferative response of the hepatocytes in male Fischer F344 rats. Dexamethasone also severely diminishes the induction and expansion of oval cells induced by the 2-acetylaminofluorene/partial hepatectomy (AAF/PH) protocol but does not have any effect on the proliferation of the bile duct cells stimulated by bile duct ligation. Dexamethasone (100 μg/kg) produces a significant decrease 59.2% of BrdU(+) hippocampal progenitor cells in Sprague–Dawley rats. Dexamethasone (100 μg/kg) decreases ERK activation in granule cell layer in Sprague–Dawley rats.
Animal Protocol
Dissolved in saline; 100 μg/kg; i.p. injection
Sprague-Dawley rats
References
Neurosci Lett.2003 Jun 26;344(2):112-6;J Physiol.2003 Feb 15;547(Pt 1):61-6.
These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C22H30FO8P.2NA
Molecular Weight
516.4
CAS #
55203-24-2
Related CAS #
55203-24-2
SMILES
O=C1C=C2CC[C@H]3[C@@H]4C[C@@H](C)[C@@](C(COP([O-])([O-])=O)=O)(O)[C@@]4(C)C[C@H](O)[C@]3(F)[C@@]2(C)C=C1.[Na+].[Na+]
Synonyms
Dexamethasone sodium phosphate
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO:<1 mg/mL
Water:103 mg/mL (199.45 mM)
Ethanol:<1 mg/mL
Solubility (In Vivo)
Saline: 30mg/kg
 (Please use freshly prepared in vivo formulations for optimal results.)
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 1.9365 mL 9.6824 mL 19.3648 mL
5 mM 0.3873 mL 1.9365 mL 3.8730 mL
10 mM 0.1936 mL 0.9682 mL 1.9365 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

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What volume of a given 10 mM stock solution is required to make 25 ml of a 25 μM solution?
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g/mol

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Note: Chemical formula is case sensitive: C12H18N3O4  c12h18n3o4
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In vivo Formulation Calculator (Clear solution)
Step 1: Enter information below (Recommended: An additional animal to make allowance for loss during the experiment)
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Calculation results

Working concentration mg/mL;

Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
             (2) Be sure to add the solvent(s) in order.

Biological Data
  • Dexamethasone Sodium Phosphate

    Effect of l-NAME on endothelin-1 (ET) sensitivity.J Physiol.2003 Feb 15;547(Pt 1):61-6.
  • Dexamethasone Sodium Phosphate

    Relaxation to acetylcholine (ACH; A) and sodium nitroprusside (SNP; B).J Physiol.2003 Feb 15;547(Pt 1):61-6.
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