Desoxycortone (DOC) is a potent agonist of the rainbow trout (Oncorhynchus mykiss) mineralocorticoid receptor (rtMR).[2]

Desoxycortone inhibited basal and LH-stimulated production of the maturation-inducing steroid (MIS, 17α,20β-dihydroxyprogesterone) in testis fragment cultures from rainbow trout. At 100 ng/ml, DOC inhibited basal MIS production by 66% and LH-stimulated MIS production by 58%.
Cortisol (a corticosteroid) also inhibited MIS production in a similar manner.
The study used an in vitro testis explant system incubated for 24 hours at 12°C.[2]

Intraperitoneal implantation of Desoxycortone (10 mg/kg) in mature male rainbow trout significantly elevated plasma DOC levels (5- to 10-fold) for at least 41 days.
DOC treatment alone did not significantly affect the initiation of spermiation (percentage of males producing milt), milt volume, seminal fluid osmolality, sodium/potassium concentrations, sperm pH, or sperm motility.
However, when co-administered with MIS (5 mg/kg), DOC significantly reduced the spermatocrit (by 26% on average), indicating increased milt fluidity/hydration.
DOC alone did not affect spermatocrit.[2]

Plasma DOC levels were measured using two radioimmunoassay (RIA) protocols with extraction and chromatographic separation.
In one protocol, plasma samples were extracted with ethyl acetate + triethylamine, purified by celite column chromatography, and then subjected to RIA using a tritiated DOC tracer and a specific rabbit anti-DOC antibody.
In another protocol, extraction was performed with cyclohexane/ethyl acetate, followed by HPLC separation on a C18 column with acetonitrile/water mobile phase, and then RIA.
Cross-reactivity with other steroids was minimal (<1.66% for corticosterone, <0.01% for MIS and cortisone).[2]

Male rainbow trout (2 years old, spring spawning strain) at the onset of spermiation were anaesthetized and implanted intraperitoneally with silastic implants containing Desoxycortone at a dose of 10 mg/kg body weight.
Implants were designed for sustained release.
Control fish received sham implants.
In some groups, DOC was co-implanted with MIS (5 mg/kg).
Fish were sampled at 2, 9, 16, 23, and 41 days post-implantation for blood collection and milt analysis.[2]

Metabolites/Metabolites 21-Hydroxyprogesterone is a known human progesterone metabolite. Mature male rainbow trout had 10 to 50 times higher plasma deoxycorticosterone (DOC) levels during sperm release compared to immature fish. In fall-spawning strains, peak DOC levels during complete sperm release were approximately 1 ng/ml. In spring-spawning strains, peak DOC levels averaged 1048 pg/ml (range 240–1800 pg/ml). Following intraperitoneal injection (10 mg/kg), plasma DOC levels remained elevated for at least 41 days (5 to 10 times higher than in the control group). [2]

No significant toxicity or mortality was reported after intraperitoneal administration of 10 mg/kg deoxycorticosterone to rainbow trout for 41 days. [2]

[1]. Action of new steroids in blocking effects of aldosterone and desoxycorticosterone on salt. Science. 1957 Nov 15;126(3281):1015-6.

[2]. Plasma 11-deoxycorticosterone (DOC) and mineralocorticoid receptor testicular expression during rainbow trout Oncorhynchus mykiss spermiation: implication with 17alpha, 20beta-dihydroxyprogesterone on the milt fluidity? Reprod Biol Endocrinol. 2008 May 19;6:19.

[3]. 11-deoxycorticosterone is a potent agonist of the rainbow trout (Oncorhynchus mykiss) mineralocorticoid receptor. Endocrinology. 2005 Jan;146(1):47-55.

[4]. First evidence of the possible implication of the 11-deoxycorticosterone (DOC) in immune activity of Eurasian perch (Perca fluviatilis, L.): comparison with cortisol. Comp Biochem Physiol A Mol Integr Physiol. 2013 Jun;165(2):149-58.

11-Deoxycorticosterone is a mineralocorticoid hormone formed by the substitution of a hydroxyl group at the 21-position of progesterone. It is a metabolite in both humans and mice. It is a mineralocorticoid, a 3-oxo-Δ⁴ steroid, a 20-oxo steroid, a 21-hydroxy steroid, and a primary α-hydroxy ketone. Its function is related to progesterone. Deoxycorticosterone has been reported in Abedus huxleyi mice, Homo sapiens, and other organisms with relevant data. Deoxycorticosterone is a C-21 steroid hormone synthesized by the adrenal glands, a precursor to cortisol and aldosterone, and possesses potential mineralocorticoid activity. Deoxycorticosterone is a steroid or mineralocorticoid hormone secreted by the zona fasciculata of the adrenal cortex. It is a precursor to aldosterone. Deoxycorticosterone is not the primary secretory hormone. It is produced from progesterone by 21β-hydroxylase, which then converts it to corticosterone by 11β-hydroxylase. Corticosterone is subsequently converted to aldosterone by aldosterone synthase. Deoxycorticosterone stimulates the collecting ducts of the kidneys, causing them to continue excreting potassium, similar to aldosterone. Deoxycorticosterone has approximately 1/20 the sodium retention capacity and 1/5 the potassium excretion capacity of aldosterone. Deoxycorticosterone can be used to treat adrenal insufficiency. Specifically, deoxycorticosterone acetate (DOCA) is used as a replacement therapy for Addison's disease. It is a steroid metabolite, an 11-deoxy derivative of corticosterone and a 21-hydroxy derivative of progesterone. See also: deoxycorticosterone acetate (its active fraction), deoxycorticosterone neovalerate (active ingredient). Deoxycorticosterone is a corticosteroid and a potential physiological ligand for the mineralocorticoid receptor (rtMR) in rainbow trout. Plasma levels are significantly elevated during sperm release in mature male rainbow trout.
rtMR is expressed in testicular somatic cells (Steeling cells and/or peritubular cells) and vas deferens epithelium.
DOC may interact with the MIS signaling pathway, thereby regulating semen fluidity/hydration during sperm release.
DOC can also inhibit testicular MIS production in vitro, suggesting a possible feedback mechanism.
This study suggests that DOC plays a role in the reproduction of male bony fish, particularly in controlling water and ion exchange during sperm hydration. [2]

C21H30O3

330.47

330.219

64-85-7

6166

White to off-white solid powder

1.15 g/cm3

488ºC at 760 mmHg

138-144 °C

263.1ºC

1.559

3.695

1

3

2

24

606

6

C[C@]12CC[C@H]3[C@H]([C@@H]1CC[C@@H]2C(=O)CO)CCC4=CC(=O)CC[C@]34C

ZESRJSPZRDMNHY-YFWFAHHUSA-N

InChI=1S/C21H30O3/c1-20-9-7-14(23)11-13(20)3-4-15-16-5-6-18(19(24)12-22)21(16,2)10-8-17(15)20/h11,15-18,22H,3-10,12H2,1-2H3/t15-,16-,17-,18+,20-,21-/m0/s1

(8S,9S,10R,13S,14S,17S)-17-(2-hydroxyacetyl)-10,13-dimethyl-1,2,6,7,8,9,11,12,14,15,16,17-dodecahydrocyclopenta[a]phenanthren-3-one

Desoxycortone Desoxycorticosteronum Desoxycorticosterone

2934.99.9001

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

DMSO : ≥ 250 mg/mL (~756.52 mM)

Solubility in Formulation 1: ≥ 2.5 mg/mL (7.57 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL.
Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.

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Solubility in Formulation 2: ≥ 2.5 mg/mL (7.57 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.

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Solubility in Formulation 3: ≥ 2.5 mg/mL (7.57 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.

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 (Please use freshly prepared in vivo formulations for optimal results.)