| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| 100mg | |||
| Other Sizes |
| Targets |
DNA and DNA polymerases. Deoxypseudouridine is a nucleotide analog that can be incorporated into DNA oligonucleotides during synthesis. Restriction enzymes react with modified DNA substrates containing deoxypseudouridine.
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| ln Vitro |
Deoxypseudouridine is an analog of a nucleoside. The restriction enzyme reacts with the enzyme in the presence of modified DNA substrates, such as deoxypseudouridine, as demonstrated by the gel's ethidium bromide staining, which revealed that the vector DNA was digested to the same extent in the control DNA sample and similar DNA samples. In the presence, unmodified (control) DNA is just as active. DNA replaced by deoxypseudouridine or deoxytuberculin is more efficiently restricted by enzymes lacking AT base pairs in their recognition sequences compared to controls [1].
Deoxypseudouridine is a nucleotide analog. It can be incorporated into DNA oligonucleotides during synthesis, resulting in modified DNA. Restriction enzymes react with modified DNA substrates containing deoxypseudouridine. |
| ln Vivo |
No specific in vivo activity data is available for Deoxypseudouridine. As a nucleotide analog, it may be used in studies of DNA synthesis, repair, or as a tool for introducing modified bases into DNA.
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| Enzyme Assay |
In vitro assays for Deoxypseudouridine typically involve its incorporation into DNA oligonucleotides during solid-phase synthesis. Modified DNA can be used in restriction enzyme digestion assays to study enzyme interactions with modified substrates. The compound may also be used in polymerase assays to study incorporation kinetics.
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| Cell Assay |
The in vitro cellular activity of Deoxypseudouridine is not typically studied, as it is a nucleotide analog used in DNA synthesis rather than a cell-active compound. It may be used in cell-based studies to investigate the effects of modified DNA on gene expression or DNA repair.
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| Animal Protocol |
No specific in vivo animal experimental protocols are available for Deoxypseudouridine. As a nucleotide analog, it is not typically administered to animals.
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| ADME/Pharmacokinetics |
Deoxypseudouridine has a molecular formula of C9H12N2O5 and a molecular weight of 228.20. It is a modified nucleoside analog of uridine with a C-glycosidic bond. IUPAC name: 5-((2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl)-1,2,3,4-tetrahydropyrimidine-2,4-dione.
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| Toxicity/Toxicokinetics |
No specific toxicological data is available for Deoxypseudouridine. As a nucleotide analog, it is expected to have low toxicity. Standard laboratory safety precautions should be observed.
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| References | |
| Additional Infomation |
Deoxypseudouridine is a modified nucleoside analog of uridine with a C-glycosidic bond. It can be incorporated into DNA oligonucleotides to generate modified DNA. It is not approved for clinical use and is intended for research purposes only.
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| Molecular Formula |
C9H12N2O5
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|---|---|
| Molecular Weight |
228.20200
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| Exact Mass |
228.075
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| CAS # |
39967-60-7
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| Related CAS # |
Deoxypseudouridine; 39967-60-7
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| PubChem CID |
10466304
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| Appearance |
White to off-white solid powder
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| LogP |
-1.8
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
16
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| Complexity |
354
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| Defined Atom Stereocenter Count |
3
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| SMILES |
C1[C@@H]([C@H](O[C@H]1C2=CNC(=O)NC2=O)CO)O
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| InChi Key |
ASOJEESZSWWNQK-RRKCRQDMSA-N
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| InChi Code |
InChI=1S/C9H12N2O5/c12-3-7-5(13)1-6(16-7)4-2-10-9(15)11-8(4)14/h2,5-7,12-13H,1,3H2,(H2,10,11,14,15)/t5-,6+,7+/m0/s1
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| Chemical Name |
5-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-1H-pyrimidine-2,4-dione
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~37 mg/mL (~162.14 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (10.96 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (10.96 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (10.96 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.3821 mL | 21.9106 mL | 43.8212 mL | |
| 5 mM | 0.8764 mL | 4.3821 mL | 8.7642 mL | |
| 10 mM | 0.4382 mL | 2.1911 mL | 4.3821 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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