| Size | Price | Stock | Qty |
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| 250mg |
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Purity: ≥98%
Dehydroepiandrosterone (trans-Dehydroandrosterone; DHEA; Prasterone; Psicosterone; EL-10; GL-701; IP-1001; NSC-9896; PB-007; SH-K-04828; Androstenolone; Diandrone) is an endogenous steroidal hormone acting as an androgen receptor antagonist and an estrogen receptor agonist. DHEA is acts as a metabolic intermediate in the biosynthesis of estrogen and androgen. Also, DHEA has a variety of potential biological effects by binding to nuclear and cell surface receptors and acts as a neurosteroid. DHEA significantly increased neural stem cells growth when grew with leukemia inhibitory factor and EGF.
| ln Vitro |
DHEA (Prasterone) is a potent antiapoptotic agent, correcting the serum deprivation-induced apoptosis in prostate cancer cells (DU145 and LNCaP cell lines) as well as in colon cancer cells (Caco2 cell line). DHEA (Prasterone) significantly reduces serum deprivation- induced apoptosis in all 3 cancer cell types, quantitated with the APOPercentage assay (apoptosis is reduced from 0.587±0.053 to 0.142±0.0016 or 0.059±0.002 after treatment for 12 hours with DHEA or NGF, respectively; n=3, P<0.01), and by flow cytometry analysis (FACS) for DU145 cells. The antiapoptotic activity of DHEA is dose dependent with an EC50 at nanomolar doses (EC50: 11.2±3.6 nM and 12.4±2.2 nM in DU145 and Caco2 cells, respectively)[1] DHEA (Prasterone) is the major sex steroid precursor in humans and can be converted directly to androgens. DHEA (Prasterone) (≥1 μM) produces a dose-dependent suppression of Chub-S7 proliferation, as determined by thymidine incorporation tests. (Prasterone) administration decreases expression of the important glucocorticoid-regulating genes H6PDH (≥100 nM) and HSD11B1 (≥1 μM) in differentiating preadipocytes in a dose-dependent manner. In accord with this discovery, DHEA (Prasterone) treatment (≥1 μM) leads in a considerable reduction in 11β-HSD1 oxoreductase activity (≥1 μM) and a contemporaneous increase in dehydrogenase activity at the highest DHEA dose utilized (25 μM DHEA) in differentiated adipocytes[2].
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| ln Vivo |
When male B6 mice (groups of five mice) were given DHEA (Prasterone) in their diet (0.45% w/w) for eight weeks, their body temperatures significantly decreased as compared to mice given the control AIN-76A diet. Comparing control and pair-fed mice revealed significant differences as well. 26 out of 29 times that the animals were tested, the mice fed DHEA (Prasterone) had significantly lower temperatures than the mice fed the control diet; the mice fed in pairs to the DHEA (Prasterone) group showed less of an impact, with 8 out of 29 values being significantly lower than the mice fed AIN-76A ad libitum. There is a significant difference in the temperatures of mice fed DHEA (Prasterone) or in pairs fed DHEA (Prasterone) across 21 out of 29 tests. Mice fed the control diet had much larger body weights than mice fed DHEA or mice fed in pairs to DHEA (Prasterone). With the exception of Week 9 (n=3), the average daily food intake (grams) from cages is calculated for each week (n=7). When mice are fed DHEA (Prasterone), their food intake is markedly reduced. Mice pairs that were fed DHEA (Prasterone) were intended to eat roughly the same amount. Therefore, it would seem that both a different mechanism and food restriction are how DHEA (Prasterone) lowers body temperature[3].
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| References |
[1]. Anagnostopoulou V, et al. Differential effects of dehydroepiandrosterone and testosterone in prostate and colon cancer cell apoptosis: the role of nerve growth factor (NGF) receptors. Endocrinology. 2013 Jul;154(7):2446-56.
[2]. McNelis JC, et al. Dehydroepiandrosterone exerts anti-glucocorticoid action on human preadipocyte proliferation, differentiation and glucose uptake. Am J Physiol Endocrinol Metab. 2013 Nov 1;305(9):E1134-44. [3]. Catalina F, et al. Decrease of core body temperature in mice by dehydroepiandrosterone. Exp Biol Med (Maywood). 2002 Jun;227(6):382-8 |
| Molecular Formula |
C19H28O2
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| Molecular Weight |
288.43
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| CAS # |
53-43-0
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| Related CAS # |
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| SMILES |
O=C1CC[C@@]2([H])[C@]3([H])CC=C4C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@@]21C
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| InChi Key |
FMGSKLZLMKYGDP-USOAJAOKSA-N
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| InChi Code |
InChI=1S/C19H28O2/c1-18-9-7-13(20)11-12(18)3-4-14-15-5-6-17(21)19(15,2)10-8-16(14)18/h3,13-16,20H,4-11H2,1-2H3/t13-,14-,15-,16-,18-,19-/m0/s1
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| Chemical Name |
(3S,8R,9S,10R,13S,14S)-3-hydroxy-10,13-dimethyl-1,2,3,4,7,8,9,10,11,12,13,14,15,16-tetradecahydro-17H-cyclopenta[a]phenanthren-17-one
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| Synonyms |
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
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| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.4670 mL | 17.3352 mL | 34.6705 mL | |
| 5 mM | 0.6934 mL | 3.4670 mL | 6.9341 mL | |
| 10 mM | 0.3467 mL | 1.7335 mL | 3.4670 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Proc Natl Acad Sci U S A. 1998 Feb 17;95(4):1852-7. |
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The effects of DHEA on the lesions induced by NMDA infused into the hippocampus of rats. Proc Natl Acad Sci U S A. 1998 Feb 17;95(4):1852-7. |
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