| Size | Price | Stock | Qty |
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| 5mg |
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DCLK1-IN-1 is a novel and potent chemical probe for the doublecortin like kinase 1 (DCLK1 kinase) domain, may be used to investigate DCLK1 biology and establish its role in cancer, like DCLK1+ pancreatic ductal adenocarcinoma (PDAC).
| ln Vitro |
For ERK5, ACK, and LRRK2, DCLK1-IN-1 did not exhibit any discernible activity. HCT116 cells exhibit excellent binding of DCLK1-IN-1 (IC50=279 nM) to DCLK1. The DCLK1-IN-1 compound strongly inhibits DCLK1 and weakly inhibits ERK5 in the mitochondria of PATU-8988T cells and live cells [1].
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| ln Vivo |
Inside the mold, DCLK1-IN-1 has favorable pharmacokinetic properties, including an 81% epidermal bioavailability, an area under contour of 5506 h/ng mL, and a half-life of 2.09 hours [1].
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| References |
| Molecular Formula |
C26H28F3N7O2
|
|---|---|
| Molecular Weight |
527.541435241699
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| Exact Mass |
527.225
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| CAS # |
2222635-15-4
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| PubChem CID |
134457640
|
| Appearance |
Light yellow to yellow solid powder
|
| LogP |
4.2
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| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
11
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
38
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| Complexity |
812
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| Defined Atom Stereocenter Count |
0
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| SMILES |
FC(CN1C(C2C=CC=CC=2N(C)C2=C1C=NC(=N2)NC1C=CC(=CC=1OC)N1CCN(C)CC1)=O)(F)F
|
| InChi Key |
OQFCHSFVWSLDAO-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C26H28F3N7O2/c1-33-10-12-35(13-11-33)17-8-9-19(22(14-17)38-3)31-25-30-15-21-23(32-25)34(2)20-7-5-4-6-18(20)24(37)36(21)16-26(27,28)29/h4-9,14-15H,10-13,16H2,1-3H3,(H,30,31,32)
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| Chemical Name |
2-[2-methoxy-4-(4-methylpiperazin-1-yl)anilino]-11-methyl-5-(2,2,2-trifluoroethyl)pyrimido[4,5-b][1,4]benzodiazepin-6-one
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| Synonyms |
DCLK1IN1 DCLK1 IN 1
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~35.71 mg/mL (~67.69 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (3.94 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (3.94 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.8956 mL | 9.4780 mL | 18.9559 mL | |
| 5 mM | 0.3791 mL | 1.8956 mL | 3.7912 mL | |
| 10 mM | 0.1896 mL | 0.9478 mL | 1.8956 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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