| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| ln Vitro |
DC260126 suppresses palmitic acid potentiated glucose-stimulated insulin secretion, decreases GTP-loading and ERK1/2 phosphorylation stimulated by linoleic acid in GPR40-CHO cells, and negatively regulates GPR40 mRNA expression induced by oleic acid in Min6 cells[1].
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| Animal Protocol |
Mice: Male C57BL/KsJ-Lepdb (db/db) animals are kept in a 12-hour light-dark cycle at 23°C with free access to water and a regular diet of chow. Nine-week-old male db/db mice are split into four groups (n=6) to examine the dose-dependent effect of DC260126. Using a tail vein injection, mice are given either DC260126 (3, 10, 30 mg/kg) or vehicle (5% DMSO in PBS) once a day for five days. On day five, all mouse groups undergo a 6-hour fast, after which blood samples are taken from the orbital venous plexus and separated by centrifugation to extract serum. Next, an ELISA kit is used to measure the serum insulin concentration. In these long-term studies, eighteen six-week-old male, obese db/db mice are placed into two groups and given either DC260126 (10 mg/kg) or vehicle (5% DMSO in PBS) once a day by tail vein injection for 24 days.
Rats: Zucker rats that are obese and female (fa/fa) are kept in a 12:12 light-dark cycle, given free access to water, and fed a high-fat diet consisting of 15% fat, 1% cholesterol, 0.5% sodium cholate, and 15% sucrose, with the exception of times when they are fasted prior to certain experiments. Based on body weight, two groups of six rats each are created at eight weeks of age. During eight weeks, rats receive intraperitoneal injections of DC260126 (6 mg/kg) or vehicle (propylene glycol) once a day. Periodically, food consumption and body weight are assessed. Following a 12-hour fast at the conclusion of the experiment, blood is drawn from the mice. Adipose, renal, and liver tissues are quickly removed and weighed. For western blotting analysis, liver samples are kept at -80°C after being snap frozen in liquid nitrogen. |
| References |
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| Molecular Formula |
C₁₆H₁₈FNO₂S
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|---|---|
| Molecular Weight |
307.3834
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| Exact Mass |
307.1
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| Elemental Analysis |
C, 62.52 H, 5.90 F, 6.18 N, 4.56 O, 10.41 S, 10.43
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| CAS # |
346692-04-4
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| Appearance |
Solid powder
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| SMILES |
CCCCC1=CC=C(C=C1)NS(=O)(=O)C2=CC=C(C=C2)F
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| InChi Key |
CNGHPXKWPGIDSK-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C16H18FNO2S/c1-2-3-4-13-5-9-15(10-6-13)18-21(19,20)16-11-7-14(17)8-12-16/h5-12,18H,2-4H2,1H3
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| Chemical Name |
N-(4-butylphenyl)-4-fluorobenzenesulfonamide
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| Synonyms |
DC 260126; DC-260126; DC260126
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~61 mg/mL (~198.5 mM)
Ethanol: ~61 mg/mL |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (8.13 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.13 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (8.13 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.2533 mL | 16.2665 mL | 32.5330 mL | |
| 5 mM | 0.6507 mL | 3.2533 mL | 6.5066 mL | |
| 10 mM | 0.3253 mL | 1.6267 mL | 3.2533 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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