| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
|
||
| Other Sizes |
| Targets |
Not applicable (linker/reagent).
|
|---|---|
| ln Vitro |
The triazole bond can be stabilized by a copper-free click chemistry reaction between the scaffold base and the azide group.
DBCO-PEG4-biotin is a click chemistry reagent. The DBCO group enables strain-promoted alkyne-azide cycloaddition (SPAAC) with azide-containing molecules. The biotin moiety allows for high-affinity binding to streptavidin or avidin for detection or purification. The PEG4 spacer improves water solubility. |
| ln Vivo |
DBCO-PEG4-biotin is not a drug and has no direct in vivo activity. It is used as a chemical tool for labeling biomolecules in vitro and in vivo for detection or purification purposes.
|
| Enzyme Assay |
DBCO-PEG4-biotin is used in chemical reactions to demonstrate copper-free click chemistry and biotin-streptavidin binding. The reaction between DBCO-PEG4-biotin and azide-containing molecules is monitored by analytical techniques such as HPLC or mass spectrometry. Biotinylation is confirmed by streptavidin binding assays.
|
| Cell Assay |
DBCO-PEG4-biotin is used in cell-based assays for labeling azide-modified biomolecules on cell surfaces or within cells. Cells are incubated with azide-containing probes, followed by DBCO-PEG4-biotin, and labeling is detected by streptavidin-conjugated fluorophores or enzymes.
|
| Animal Protocol |
DBCO-PEG4-biotin is used in animal models for in vivo labeling and imaging of azide-modified biomolecules. The biotin moiety allows for detection using streptavidin-conjugated probes. The click chemistry enables efficient and bioorthogonal labeling in vivo.
|
| ADME/Pharmacokinetics |
No detailed PK data available. DBCO-PEG4-biotin has a molecular weight of 749.92. Soluble in DMSO and ethanol (100 mM). Purity ≥95%. Storage at -20°C.
|
| Toxicity/Toxicokinetics |
No detailed toxicity data available. For research use only.
|
| References | |
| Additional Infomation |
DBCO-PEG4-biotin is a biotinylation reagent. Molecular formula: C39H51N5O8S; MW: 749.92. For research use only.
|
| Molecular Formula |
C39H51N5O8S
|
|---|---|
| Molecular Weight |
749.915949106216
|
| Exact Mass |
749.345
|
| CAS # |
1255942-07-4
|
| PubChem CID |
91757917
|
| Appearance |
Off-white to yellow solid powder
|
| Density |
1.3±0.1 g/cm3
|
| Boiling Point |
1062.4±65.0 °C at 760 mmHg
|
| Flash Point |
596.3±34.3 °C
|
| Vapour Pressure |
0.0±0.3 mmHg at 25°C
|
| Index of Refraction |
1.620
|
| LogP |
1.72
|
| Hydrogen Bond Donor Count |
4
|
| Hydrogen Bond Acceptor Count |
9
|
| Rotatable Bond Count |
23
|
| Heavy Atom Count |
53
|
| Complexity |
1240
|
| Defined Atom Stereocenter Count |
3
|
| SMILES |
C1[C@H]2[C@@H]([C@@H](S1)CCCCC(=O)NCCOCCOCCOCCOCCC(=O)NCCC(=O)N3CC4=CC=CC=C4C#CC5=CC=CC=C53)NC(=O)N2
|
| InChi Key |
LNHSQAOQVNHUGL-QRBHCBQLSA-N
|
| InChi Code |
InChI=1S/C39H51N5O8S/c45-35(12-6-5-11-34-38-32(28-53-34)42-39(48)43-38)41-18-20-50-22-24-52-26-25-51-23-21-49-19-16-36(46)40-17-15-37(47)44-27-31-9-2-1-7-29(31)13-14-30-8-3-4-10-33(30)44/h1-4,7-10,32,34,38H,5-6,11-12,15-28H2,(H,40,46)(H,41,45)(H2,42,43,48)/t32-,34-,38-/m0/s1
|
| Chemical Name |
5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-N-[2-[2-[2-[2-[3-[[3-(2-azatricyclo[10.4.0.04,9]hexadeca-1(16),4,6,8,12,14-hexaen-10-yn-2-yl)-3-oxopropyl]amino]-3-oxopropoxy]ethoxy]ethoxy]ethoxy]ethyl]pentanamide
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~133.35 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (3.33 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (3.33 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (3.33 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.3335 mL | 6.6674 mL | 13.3348 mL | |
| 5 mM | 0.2667 mL | 1.3335 mL | 2.6670 mL | |
| 10 mM | 0.1333 mL | 0.6667 mL | 1.3335 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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