| Size | Price | Stock | Qty |
|---|---|---|---|
| 10mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
Not applicable (linker/reagent).
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|---|---|
| ln Vitro |
The ADC is made up of a preamplifier, to which the ADC input connects the ADC cytotoxic.
DBCO-NHS ester is a bifunctional linker. The NHS ester group reacts with primary amines to form stable amide bonds. The DBCO group enables strain-promoted alkyne-azide cycloaddition (SPAAC) with azide-containing molecules. This allows for the conjugation of two different moieties. |
| ln Vivo |
DBCO-NHS ester is not a drug and has no direct in vivo activity. It is used as a chemical tool for the synthesis of bioconjugates, including antibody-drug conjugates (ADCs), for in vivo applications.
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| Enzyme Assay |
DBCO-NHS ester is used in chemical reactions to demonstrate NHS ester-amine coupling. The reaction is monitored by analytical techniques such as HPLC or mass spectrometry to confirm conjugation efficiency.
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| Cell Assay |
DBCO-NHS ester is used in cell-based assays for labeling amine-containing biomolecules on cell surfaces. Cells are treated with DBCO-NHS ester, followed by azide-containing probes, and labeling is detected by fluorescence or other methods.
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| Animal Protocol |
DBCO-NHS ester is used in animal models as part of ADC or bioconjugate formulations for targeted therapy. The NHS ester enables conjugation to antibodies, and the DBCO group enables attachment of payloads via click chemistry.
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| ADME/Pharmacokinetics |
No detailed PK data available. DBCO-NHS ester has a molecular weight of 402.40. Soluble in DMSO. Purity ≥97%. Storage at -20°C.
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| Toxicity/Toxicokinetics |
No detailed toxicity data available. For research use only.
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| References | |
| Additional Infomation |
DBCO-NHS ester is an amine-reactive click chemistry reagent. Molecular formula: C23H18N2O5. MW: 402.40. For research use only.
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| Molecular Formula |
C23H18N2O5
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|---|---|
| Molecular Weight |
402.3994
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| Exact Mass |
402.122
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| CAS # |
1353016-71-3
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| PubChem CID |
77078178
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| Appearance |
White to yellow solid powder
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| Density |
1.43±0.1 g/cm3
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| Boiling Point |
670.2±65.0 °C at 760 mmHg
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| LogP |
2.323
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
30
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| Complexity |
778
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| Defined Atom Stereocenter Count |
0
|
| InChi Key |
XCEBOJWFQSQZKR-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C23H18N2O5/c26-20(13-14-23(29)30-25-21(27)11-12-22(25)28)24-15-18-7-2-1-5-16(18)9-10-17-6-3-4-8-19(17)24/h1-8H,11-15H2
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| Chemical Name |
(2,5-dioxopyrrolidin-1-yl) 4-(2-azatricyclo[10.4.0.04,9]hexadeca-1(16),4,6,8,12,14-hexaen-10-yn-2-yl)-4-oxobutanoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~124.25 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.21 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (6.21 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (6.21 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4851 mL | 12.4254 mL | 24.8509 mL | |
| 5 mM | 0.4970 mL | 2.4851 mL | 4.9702 mL | |
| 10 mM | 0.2485 mL | 1.2425 mL | 2.4851 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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