| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| 250mg | |||
| 500mg | |||
| Other Sizes |
| Targets |
Not applicable (linker/reagent).
|
|---|---|
| ln Vitro |
The ADC is made up of a preamplifier, to which the ADC input connects the ADC cytotoxic.
DBCO-Maleimide is a bifunctional linker. The maleimide group reacts with sulfhydryl groups (e.g., cysteine residues on antibodies) to form stable thioether bonds. The DBCO group enables strain-promoted alkyne-azide cycloaddition (SPAAC) with azide-containing molecules. This allows for the conjugation of two different moieties via a stable linker. |
| ln Vivo |
DBCO-Maleimide is not a drug and has no direct in vivo activity. It is used as a chemical tool for the synthesis of bioconjugates, including antibody-drug conjugates (ADCs), for in vivo applications.
|
| Enzyme Assay |
DBCO-Maleimide is used in chemical reactions to demonstrate maleimide-thiol coupling and copper-free click chemistry. The reactions are monitored by analytical techniques such as HPLC or mass spectrometry to confirm conjugation efficiency.
|
| Cell Assay |
DBCO-Maleimide is used in cell-based assays for labeling sulfhydryl-containing biomolecules on cell surfaces. Cells are treated with DBCO-Maleimide, followed by azide-containing probes, and labeling is detected by fluorescence or other methods.
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| Animal Protocol |
DBCO-Maleimide is used in animal models as part of ADC or bioconjugate formulations for targeted therapy. The maleimide enables conjugation to antibodies (via cysteine), and the DBCO group enables attachment of payloads via click chemistry.
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| ADME/Pharmacokinetics |
No detailed PK data available. DBCO-Maleimide has a molecular weight of 427.45. Purity 95%. Storage at -20°C.
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| Toxicity/Toxicokinetics |
No detailed toxicity data available. For research use only.
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| References | |
| Additional Infomation |
DBCO-Maleimide is an ADC linker. Molecular formula: C25H21N3O4; MW: 427.45. For research use only.
|
| Molecular Formula |
C25H21N3O4
|
|---|---|
| Molecular Weight |
427.4519
|
| Exact Mass |
427.153
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| CAS # |
1395786-30-7
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| PubChem CID |
77078149
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| Appearance |
Light yellow to brown solid powder
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| Density |
1.4±0.1 g/cm3
|
| Boiling Point |
790.5±60.0 °C at 760 mmHg
|
| Flash Point |
431.9±32.9 °C
|
| Vapour Pressure |
0.0±2.8 mmHg at 25°C
|
| Index of Refraction |
1.688
|
| LogP |
3.1
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
6
|
| Heavy Atom Count |
32
|
| Complexity |
849
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
C1C2=CC=CC=C2C#CC3=CC=CC=C3N1C(=O)CCNC(=O)CCN4C(=O)C=CC4=O
|
| InChi Key |
NHFQNAGPXIVKND-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C25H21N3O4/c29-22(14-16-27-23(30)11-12-24(27)31)26-15-13-25(32)28-17-20-7-2-1-5-18(20)9-10-19-6-3-4-8-21(19)28/h1-8,11-12H,13-17H2,(H,26,29)
|
| Chemical Name |
N-[3-(2-azatricyclo[10.4.0.04,9]hexadeca-1(16),4,6,8,12,14-hexaen-10-yn-2-yl)-3-oxopropyl]-3-(2,5-dioxopyrrol-1-yl)propanamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~125 mg/mL (~292.43 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (4.87 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (4.87 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (4.87 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.3395 mL | 11.6973 mL | 23.3945 mL | |
| 5 mM | 0.4679 mL | 2.3395 mL | 4.6789 mL | |
| 10 mM | 0.2339 mL | 1.1697 mL | 2.3395 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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