| Size | Price | Stock | Qty |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
DAPI binds to DNA, specifically to the minor groove of A-T rich sequences. It binds preferentially to DNA rich in adenine and thymine. The compound forms a fluorescent complex upon binding to DNA, enabling detection and visualization of DNA in cells and chromosomes.
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| ln Vitro |
DAPI binds to the double-stranded DNA's minor groove, preferring to attach to adenine-thymine clusters. Permeabilization and/or fixation of cells are necessary for DAPI to penetrate and bind DNA. Approximately a 20-fold increase in fluorescence is observed upon DAPI binding to double-stranded DNA. [1]
DAPI binds preferentially to DNA rich in adenine and thymine. It forms a fluorescent complex by attaching in the minor groove of A-T rich sequences. The compound is cell-permeable and is used to stain DNA and chromosomes for fluorescent microscopy and flow cytometry. |
| ln Vivo |
DAPI is used as a fluorescent probe to stain DNA and chromosomes in cells. It binds preferentially to DNA rich in adenine and thymine. The compound is cell-permeable and can be used in live and fixed cells. It is commonly used in flow cytometry and fluorescence microscopy applications.
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| Enzyme Assay |
In vitro assays for DAPI involve its use as a fluorescent DNA stain. The compound is incubated with DNA samples, and fluorescence is measured to quantify DNA content. DAPI binds preferentially to A-T rich DNA sequences. The compound's fluorescence properties are characterized by excitation and emission spectra.
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| Cell Assay |
DAPI is used in cell-based assays to stain DNA and chromosomes. Cells are incubated with DAPI, and fluorescence microscopy or flow cytometry is used to visualize and quantify DNA content. The compound is cell-permeable and binds preferentially to A-T rich DNA sequences.
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| Animal Protocol |
DAPI is used in animal studies for staining DNA and chromosomes in tissue sections. The compound is cell-permeable and can be used to visualize DNA in tissues. However, specific animal study protocols are not well-documented. DAPI is primarily used as a research tool for DNA staining.
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| ADME/Pharmacokinetics |
DAPI has a molecular weight of 350.25 and a molecular formula of C16H17Cl2N5. It has a CAS number of 28718-90-3. The compound is a cell-permeable fluorescent probe. It binds preferentially to DNA rich in adenine and thymine. It is soluble in water and DMSO. It should be stored at -20°C.
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| Toxicity/Toxicokinetics |
No detailed toxicity data is available for DAPI. The compound is for research use only and is not intended for human therapeutic use. It is a fluorescent DNA stain used in laboratory applications. It is generally considered safe for research purposes when handled properly.
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| References | |
| Additional Infomation |
DAPI (4',6-diamidino-2-phenylindole) dihydrochloride is a cell-permeable DNA-binding dye that binds preferentially to DNA rich in adenine and thymine. It has a molecular weight of 350.25 and a molecular formula of C16H17Cl2N5. The compound has a CAS number of 28718-90-3. It is a fluorescent probe commonly used to stain DNA and chromosomes for fluorescent microscopy and flow cytometry applications. It forms a fluorescent complex by attaching in the minor groove of A-T rich sequences. The compound is for research use only and has not been approved for clinical use.
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| Molecular Formula |
C16H17CL2N5
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|---|---|
| Molecular Weight |
350.24
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| Exact Mass |
349.086
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| Elemental Analysis |
C, 54.87; H, 4.89; Cl, 20.24; N, 20.00
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| CAS # |
28718-90-3
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| Related CAS # |
47165-04-8
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| PubChem CID |
160166
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| Appearance |
Light yellow to green yellow solid powder
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| Density |
1.41g/cm3
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| Boiling Point |
545.4ºC at 760mmHg
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| Melting Point |
>300°C (dec.)
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| Flash Point |
283.6ºC
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| Vapour Pressure |
5.96E-12mmHg at 25°C
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| LogP |
5.607
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| Hydrogen Bond Donor Count |
7
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
23
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| Complexity |
413
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| Defined Atom Stereocenter Count |
0
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| SMILES |
Cl[H].Cl[H].N1([H])C(C2C([H])=C([H])C(/C(=N/[H])/N([H])[H])=C([H])C=2[H])=C([H])C2C([H])=C([H])C(/C(=N/[H])/N([H])[H])=C([H])C1=2
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| InChi Key |
FPNZBYLXNYPRLR-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
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| Chemical Name |
2-(4-carbamimidoylphenyl)-1H-indole-6-carboximidamide;dihydrochloride
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| Synonyms |
FxCycle Violet; DAPI Dihydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 70~75 mg/mL (199.9~214.1 mM)
H2O: ~5.6 mg/mL (~15.9 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (5.94 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (5.94 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (5.94 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.8552 mL | 14.2759 mL | 28.5519 mL | |
| 5 mM | 0.5710 mL | 2.8552 mL | 5.7104 mL | |
| 10 mM | 0.2855 mL | 1.4276 mL | 2.8552 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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