| Size | Price | Stock | Qty |
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| 10mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
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Purity: =98.9%
| Targets |
- Mitochondrial aldehyde dehydrogenase (Ki = 0.082 μM) [3]
- Serotonin and dopamine metabolism - related enzymes in mitochondria [4] |
|---|---|
| ln Vitro |
- Inhibiting mitochondrial enzyme activity: Daidzin inhibits mitochondrial aldehyde dehydrogenase activity, with a Ki value of 0.082 μM. It also inhibits the metabolism of serotonin and dopamine in isolated mitochondria, suppressing the production of 5 - HIAA (IC50 = 2.7 μM) and increasing the content of 5 - HTOL in hamster mitochondrial culture [3][4]
Daidzin had a 0.082 and 0.052 μM Kis inhibitory effect on ALDH-2 and hamster, respectively[1]. Daidzin (500 μM) inhibits phorbol myristyl ester (PMA), which results in antioxidant action. To find out how well 5-HIAL in mitochondria and cells reduces cytochrome c, daidin (0-30 μM, 30 min) suppresses 5-HIAA (IC50 value = 2.7 μM) and increases hamster mitochondrial culture [3]. The 5-HTOL content [4]. |
| ln Vivo |
- Reducing blood alcohol levels and shortening sleep time: In rats, Daidzin (30 mg/kg, by gavage) can lower the blood alcohol levels caused by 3 g ethanol/kg body weight and shorten the sleep time produced by ethanol intoxication (7 g/kg, by gavage) [1]
- Suppressing ethanol intake: In Syrian golden hamsters, Daidzin can suppress ethanol intake. When administered at a dose of 70 meq/hamster/day by intra - peritoneal injection, it can prevent 62% of ethanol inhalation [3][4] Daidzin (70 meq/hamster/day, intraperitoneal injection) can prevent 62% of ethanol inhalation in rat hamsters [4]. Daidzin (30 mg/kg, gavage) lowers fasting reserve blood alcohol levels (caused by 3 g ethanol/kg weight) and sleep duration (produced by ethanol intoxication, gavage 7 g/kg) [3]. |
| Enzyme Assay |
- Mitochondrial aldehyde dehydrogenase activity assay: Prepare mitochondrial extracts from relevant tissues or cells. Add different concentrations of Daidzin to the reaction system, along with the substrate of mitochondrial aldehyde dehydrogenase. Incubate at an appropriate temperature for a certain period, then use a specific detection method (such as measuring the change of product concentration by spectrophotometry) to determine the enzyme activity, and calculate the inhibitory effect of Daidzin on the enzyme according to the experimental results [3]
- Serotonin and dopamine metabolism - related enzyme activity assay: Isolate mitochondria from hamster tissues. Incubate mitochondria with Daidzin at different concentrations, and add the necessary co - factors and substrates for serotonin and dopamine metabolism. After a certain incubation time, detect the levels of 5 - HIAA and 5 - HTOL in the reaction system by appropriate methods (such as high - performance liquid chromatography), and evaluate the inhibitory effect of Daidzin on the metabolism of these two substances [4] |
| Animal Protocol |
- For the experiment of reducing blood alcohol levels and shortening sleep time, rats are first fasted, and then given 3 g ethanol/kg body weight by gavage. After that, Daidzin is administered at a dose of 30 mg/kg by gavage. Observe and record the blood alcohol levels at different time points, and record the sleep time after giving 7 g/kg ethanol by gavage to induce intoxication [1]
- For the experiment of suppressing ethanol intake, Syrian golden hamsters are used. Daidzin is dissolved in an appropriate solvent and administered to hamsters by intra - peritoneal injection at a dose of 70 meq/hamster/day. Observe and record the ethanol intake of hamsters [3][4] |
| Toxicity/Toxicokinetics |
107971 mouse LD50 intraperitoneal >2 gm/kg Pharmaceutical Chemistry Journal, 13(51), 1979
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| References |
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| Additional Infomation |
Daidzin is an antioxidant isoflavonoid. It has the potential to be used as an antidipsotropic agent, which can reduce blood alcohol levels, shorten the sleep time induced by ethanol intoxication, and suppress ethanol intake, indicating its therapeutic potential for alcohol - related problems [1][3][4]
Daidzein 7-O-beta-D-glucoside is a glycosyloxyisoflavone that is daidzein attached to a beta-D-glucopyranosyl residue at position 7 via a glycosidic linkage. It is used in the treatment of alcohol dependency (antidipsotropic). It has a role as a plant metabolite. It is a hydroxyisoflavone, a monosaccharide derivative and a 7-hydroxyisoflavones 7-O-beta-D-glucoside. It is functionally related to a daidzein. Daidzin has been reported in Salvia hispanica, Begonia nantoensis, and other organisms with data available. See also: Astragalus propinquus root (part of). |
| Molecular Formula |
C21H20O9
|
|---|---|
| Molecular Weight |
416.3781
|
| Exact Mass |
416.11
|
| Elemental Analysis |
C, 60.58; H, 4.84; O, 34.58
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| CAS # |
552-66-9
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| PubChem CID |
107971
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| Appearance |
White to off-white solid powder
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| Density |
1.6±0.1 g/cm3
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| Boiling Point |
727.6±60.0 °C at 760 mmHg
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| Melting Point |
234-236°C
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| Flash Point |
259.8±26.4 °C
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| Vapour Pressure |
0.0±2.5 mmHg at 25°C
|
| Index of Refraction |
1.695
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| LogP |
0.45
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| Hydrogen Bond Donor Count |
5
|
| Hydrogen Bond Acceptor Count |
9
|
| Rotatable Bond Count |
4
|
| Heavy Atom Count |
30
|
| Complexity |
644
|
| Defined Atom Stereocenter Count |
5
|
| SMILES |
C1=CC(=CC=C1C2=COC3=C(C2=O)C=CC(=C3)O[C@H]4[C@@H]([C@H]([C@@H]([C@H](O4)CO)O)O)O)O
|
| InChi Key |
KYQZWONCHDNPDP-QNDFHXLGSA-N
|
| InChi Code |
InChI=1S/C21H20O9/c22-8-16-18(25)19(26)20(27)21(30-16)29-12-5-6-13-15(7-12)28-9-14(17(13)24)10-1-3-11(23)4-2-10/h1-7,9,16,18-23,25-27H,8H2/t16-,18-,19+,20-,21-/m1/s1
|
| Chemical Name |
3-(4-hydroxyphenyl)-7-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxychromen-4-one
|
| Synonyms |
Daidzin; 552-66-9; Daidzoside; Daidzein 7-O-glucoside; Daidzein 7-glucoside; Daidzein-7-glucoside; daidzein 7-O-beta-D-glucoside; CHEBI:42202;
|
| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~250 mg/mL (~600.41 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (5.00 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.08 mg/mL (5.00 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (5.00 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4017 mL | 12.0083 mL | 24.0165 mL | |
| 5 mM | 0.4803 mL | 2.4017 mL | 4.8033 mL | |
| 10 mM | 0.2402 mL | 1.2008 mL | 2.4017 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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