| Size | Price | Stock | Qty |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
Dabcyl acid is a dark fluorescence quencher used in FRET applications. It absorbs energy from a fluorophore without emitting fluorescence itself. The compound is used as a molecular beacon nucleic acid probe to identify and report the presence of specific nucleic acids in homogeneous solutions. It is an azo dye characterized by a benzoic acid functional group and a dimethylamino-azobenzene chromophore.
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| ln Vitro |
Dabcyl acid is a non-fluorescent chromophore and quencher. It is used as a molecular beacon nucleic acid probe. The compound efficiently absorbs and dissipates energy without fluorescence emission. When paired with a fluorophore, Dabcyl suppresses fluorescence until a specific event occurs. It is used in FRET applications and as a quencher in various scientific and biomedical applications.
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| ln Vivo |
Dabcyl acid is used primarily as a laboratory reagent rather than a therapeutic compound. Its utility in FRET applications and molecular beacon probes has been established. The compound is used in scientific and biomedical research for detecting specific nucleic acids and studying molecular interactions. Further in vivo studies are limited as the compound is primarily a reagent.
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| Enzyme Assay |
In vitro FRET assays for Dabcyl acid involve pairing the compound with a fluorophore to measure molecular interactions. The compound is conjugated to a probe molecule, and fluorescence is measured. When the fluorophore and Dabcyl are in close proximity, fluorescence is quenched. Upon a specific event (e.g., target binding), the fluorophore and Dabcyl separate, resulting in increased fluorescence. Assays are performed in appropriate buffer systems with positive controls.
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| Cell Assay |
Dabcyl acid cell-based assays are limited as the compound is primarily a labeling reagent. The compound is used in molecular beacon probes for detecting nucleic acids in cells. Cells are treated with Dabcyl-labeled probes and fluorescence is measured. Cell viability is assessed by standard assays. Experiments are performed with appropriate positive and negative controls.
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| Animal Protocol |
Dabcyl acid in vivo studies are limited as the compound is primarily a laboratory reagent. Its utility in FRET applications and molecular beacon probes has been established. The compound is used in research applications for detecting nucleic acids and studying molecular interactions. Studies are conducted in accordance with institutional guidelines for reagent use.
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| ADME/Pharmacokinetics |
Dabcyl acid (MW 269.3 g/mol, C15H15N3O2) is a non-fluorescent azo dye. It is also known as DABCYL and 4-(dimethylamino)azobenzene-4′-carboxylic acid. The compound is soluble in organic solvents. It is stable under recommended storage conditions. Dabcyl acid is used as a dark fluorescence quencher in FRET applications. Pharmacokinetic properties are not typically characterized as it is a laboratory reagent.
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| Toxicity/Toxicokinetics |
Dabcyl acid is generally considered safe as a laboratory reagent. The compound is widely used in FRET applications and molecular beacon probes. No significant adverse effects have been reported in the available literature at research-use concentrations. The compound is intended for research use only. Standard laboratory safety practices should be employed when handling this compound. Comprehensive toxicological evaluation would be required for therapeutic development.
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| Additional Infomation |
Dabcyl acid (DABCYL) is a non-fluorescent azo dye and dark fluorescence quencher used in FRET applications. It efficiently absorbs and dissipates energy without emitting fluorescence. When paired with a fluorophore, Dabcyl suppresses fluorescence until a specific event occurs. It is used as a molecular beacon nucleic acid probe. Its molecular formula is C15H15N3O2 with a molecular weight of 269.3 g/mol. All applications are limited to non-human research use.
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| Molecular Formula |
C15H15N3O2
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|---|---|
| Molecular Weight |
269.2985
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| Exact Mass |
269.116
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| CAS # |
6268-49-1
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| Related CAS # |
Dabcyl acid sodium;845-46-5
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| PubChem CID |
22650
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| Appearance |
Orange to red solid powder
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| Density |
1.17g/cm3
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| Boiling Point |
482.6ºC at 760 mmHg
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| Flash Point |
245.6ºC
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| Index of Refraction |
1.592
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| LogP |
3.866
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
20
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| Complexity |
341
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
WCKQPPQRFNHPRJ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C15H15N3O2/c1-18(2)14-9-7-13(8-10-14)17-16-12-5-3-11(4-6-12)15(19)20/h3-10H,1-2H3,(H,19,20)
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| Chemical Name |
4-[[4-(dimethylamino)phenyl]diazenyl]benzoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~17.86 mg/mL (~66.32 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: 1.79 mg/mL (6.65 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 17.9 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 1.79 mg/mL (6.65 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 17.9 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.7133 mL | 18.5667 mL | 37.1333 mL | |
| 5 mM | 0.7427 mL | 3.7133 mL | 7.4267 mL | |
| 10 mM | 0.3713 mL | 1.8567 mL | 3.7133 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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