| Size | Price | Stock | Qty |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
Dabcyl acid, SE is a labeling reagent that reacts with primary amines (e.g., lysine residues, N-termini of peptides/proteins) to form stable amide bonds. It is used as a quencher in FRET-based assays. DABCYL serves as an efficient acceptor (quencher) in the development of FRET-based nucleic acid probes and protease substrates.
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| ln Vitro |
Dabcyl acid, SE is the amino-active form of Dabcyl acid (DABCYL) and is used to prepare fluorescent probes containing DABCYL. The compound reacts with primary amines to form stable amide bonds, enabling the covalent attachment of the DABCYL quencher to peptides, proteins, or oligonucleotides.
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| ln Vivo |
As a labeling reagent, Dabcyl acid, SE is used to prepare FRET-based probes for various applications including nucleic acid detection and protease activity assays. The compound enables the development of fluorescent probes where DABCYL acts as a quencher paired with a fluorophore (e.g., EDANS, FAM). These probes are used in real-time PCR, molecular beacons, and protease substrate assays.
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| Enzyme Assay |
For labeling reactions, Dabcyl acid, SE is dissolved in an appropriate organic solvent (e.g., DMSO or DMF) and reacted with amine-containing molecules (peptides, proteins, or amine-modified oligonucleotides) in a suitable buffer (e.g., bicarbonate buffer, pH 8.5–9.0). The reaction proceeds at room temperature for 1–4 hours. Unreacted reagent is removed by dialysis, gel filtration, or HPLC. Labeling efficiency is assessed by absorbance at the DABCYL absorption maximum (~453 nm).
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| Cell Assay |
Cellular assays using Dabcyl acid, SE-labeled probes typically involve the delivery of FRET-based probes into cells via transfection, microinjection, or cell-penetrating peptide conjugation. Protease activity is measured by monitoring the increase in fluorescence upon cleavage of the probe, separating the fluorophore from the DABCYL quencher. Fluorescence is measured using a fluorescence microplate reader or flow cytometry.
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| Animal Protocol |
In vivo applications of Dabcyl acid, SE-labeled probes include the use of FRET-based probes for imaging protease activity in animal models. Probes are administered via intravenous injection or local delivery. Fluorescence imaging is performed using appropriate excitation and emission filters. The increase in fluorescence signal indicates protease activity in specific tissues or tumors.
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| ADME/Pharmacokinetics |
Dabcyl acid, SE is a small molecule with a molecular weight of 366.37. The compound is soluble in organic solvents such as DMSO and DMF. As a labeling reagent, it is stable when stored dry at –20°C. Once conjugated to biomolecules, the DABCYL-labeled probes are stable under physiological conditions for extended periods.
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| Toxicity/Toxicokinetics |
Dabcyl acid, SE is generally handled as a chemical reagent with standard laboratory safety precautions. The compound is not intended for therapeutic use. No significant toxicity has been reported for the compound itself, though the labeled probes may have specific toxicity profiles depending on the biomolecule conjugated.
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| Additional Infomation |
Dabcyl SE dye is a pyrrolidone monoazo compound that can be used as a fluorescent dye.
Dabcyl acid, SE is the amine-reactive succinimidyl ester form of Dabcyl acid (DABCYL), a widely used quencher in FRET-based probes. It is used to prepare fluorescent probes for nucleic acid detection and protease activity assays. The compound reacts with primary amines to form stable amide bonds. |
| Molecular Formula |
C19H18N4O4
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|---|---|
| Molecular Weight |
366.3706
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| Exact Mass |
366.132
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| CAS # |
146998-31-4
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| PubChem CID |
4206604
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| Appearance |
Brown to red solid powder
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| Density |
1.3±0.1 g/cm3
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| Boiling Point |
550.7±60.0 °C at 760 mmHg
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| Flash Point |
286.9±32.9 °C
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| Vapour Pressure |
0.0±1.5 mmHg at 25°C
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| Index of Refraction |
1.632
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| LogP |
2.53
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
27
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| Complexity |
575
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
IBOVDNBDQHYNJI-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C19H18N4O4/c1-22(2)16-9-7-15(8-10-16)21-20-14-5-3-13(4-6-14)19(26)27-23-17(24)11-12-18(23)25/h3-10H,11-12H2,1-2H3
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| Chemical Name |
(2,5-dioxopyrrolidin-1-yl) 4-[[4-(dimethylamino)phenyl]diazenyl]benzoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~5.26 mg/mL (~14.36 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 0.53 mg/mL (1.45 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 5.3 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 0.53 mg/mL (1.45 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 5.3 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7295 mL | 13.6474 mL | 27.2948 mL | |
| 5 mM | 0.5459 mL | 2.7295 mL | 5.4590 mL | |
| 10 mM | 0.2729 mL | 1.3647 mL | 2.7295 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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