| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
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| Other Sizes |
| Targets |
D-Ribonolactone targets E. coli β-galactosidase, an enzyme that hydrolyzes lactose into glucose and galactose. As a competitive inhibitor, it binds to the enzyme's active site and prevents substrate binding. It is also a substrate for lactonases and lactonizing enzymes, which hydrolyze it to D-ribose. Its role in ribose metabolism makes it a valuable tool for studying carbohydrate metabolism.
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|---|---|
| ln Vitro |
Ki: 26 mM (β-galactosidase)
D-Ribonolactone exhibits in vitro activity as an inhibitor of E. coli β-galactosidase with a Ki of 26 mM. Its activity is assessed in enzyme assays measuring the hydrolysis of a chromogenic or fluorogenic β-galactosidase substrate (e.g., X-gal, ONPG) in the presence of varying concentrations of the inhibitor. It also serves as a substrate for lactonases. |
| ln Vivo |
In vivo, D-ribonolactone is not typically used as a therapeutic agent. It is a sugar lactone that can be hydrolyzed to D-ribose, a fundamental sugar involved in numerous biological processes. It is utilized in the production of riboflavin (vitamin B2) and supports research in genetics, cellular biology, and metabolic regulation.
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| Enzyme Assay |
In vitro enzyme assays for D-ribonolactone involve measuring its inhibition of E. coli β-galactosidase. The enzyme is incubated with a substrate (e.g., ONPG) and varying concentrations of D-ribonolactone. The production of the chromogenic product (o-nitrophenol) is measured spectrophotometrically, and the Ki (26 mM) is determined from the inhibition kinetics. Assays for lactonase activity measure the hydrolysis of D-ribonolactone to D-ribose.
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| Cell Assay |
Cellular assays for D-ribonolactone are limited, as its primary use is as an enzyme inhibitor and substrate in biochemical assays. Its effects on cells can be studied in the context of carbohydrate metabolism. E. coli cultures can be treated with the compound to assess its effects on β-galactosidase activity and bacterial growth.
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| Animal Protocol |
In vivo animal experiments with D-ribonolactone are not extensively documented, as the compound is primarily used as a research reagent. Its role in ribose metabolism and vitamin B2 production may be studied in animal models of metabolic disorders. However, such studies are not common.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for D-ribonolactone are limited. It is a small, polar molecule with a molecular weight of 148.11 and a molecular formula of C5H8O5. It is soluble in water. Its pharmacokinetic properties are related to its role as a sugar lactone; it is hydrolyzed to D-ribose, which is then metabolized via the pentose phosphate pathway. The compound is typically stored at room temperature.
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| Toxicity/Toxicokinetics |
Comprehensive toxicological data for D-ribonolactone are limited. The compound is intended for research use only and is not approved for human therapeutic use. Standard laboratory safety precautions should be followed when handling this compound.
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| References | |
| Additional Infomation |
D-ribose-1,4-lactone is a five-membered ring structure of ribonucleotide with a D-configuration. It is a metabolite. It is both a ribonucleotide and a buty-4-lactone. Functionally, it is related to D-ribonucleic acid.
Ribonolactone is a sugar lactone and an inhibitor of E. coli β-galactosidase with a Ki of 26 mM. It is a substrate for lactonases and lactonizing enzymes, which hydrolyze it to D-ribose. The compound is utilized in the production of riboflavin (vitamin B2) and other ribose derivatives. It supports research in genetics, cellular biology, and metabolic regulation. |
| Molecular Formula |
C₅H₈O₅
|
|---|---|
| Molecular Weight |
148.11
|
| Exact Mass |
148.037
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| CAS # |
5336-08-3
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| PubChem CID |
111064
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| Appearance |
White to off-white solid powder
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| Density |
1.7±0.1 g/cm3
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| Boiling Point |
364.3±11.0 °C at 760 mmHg
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| Melting Point |
83-85ºC
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| Flash Point |
162.8±12.8 °C
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| Vapour Pressure |
0.0±1.8 mmHg at 25°C
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| Index of Refraction |
1.581
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| LogP |
-2.47
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| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
5
|
| Rotatable Bond Count |
1
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| Heavy Atom Count |
10
|
| Complexity |
145
|
| Defined Atom Stereocenter Count |
3
|
| SMILES |
C([C@@H]1[C@H]([C@H](C(=O)O1)O)O)O
|
| InChi Key |
CUOKHACJLGPRHD-BXXZVTAOSA-N
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| InChi Code |
InChI=1S/C5H8O5/c6-1-2-3(7)4(8)5(9)10-2/h2-4,6-8H,1H2/t2-,3-,4-/m1/s1
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| Chemical Name |
(3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-one
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| Synonyms |
DRibonolactone; D Ribonolactone
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~125 mg/mL (~843.97 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (14.04 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (14.04 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (14.04 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.7517 mL | 33.7587 mL | 67.5174 mL | |
| 5 mM | 1.3503 mL | 6.7517 mL | 13.5035 mL | |
| 10 mM | 0.6752 mL | 3.3759 mL | 6.7517 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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