| Size | Price | Stock | Qty |
|---|---|---|---|
| 5g |
|
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| Other Sizes |
| ln Vitro |
D-(+)-Melezitose (hydrate) was used as a key substrate in the assimilation test to assist in classifying 122 clinical Klebsiella isolates from 120 patients. The assimilation ability of D-(+)-Melezitose (hydrate) differed across Klebsiella species: when combined with growth at 10°C and histamine assimilation tests, it enabled 84% of isolates to be identified as Klebsiella pneumoniae or Klebsiella variicola, 15% as Klebsiella oxytoca, and 1% as Raoultella planticola. This phenotypic identification result was fully validated by the reference method of 512-bp rpoB gene sequence analysis, which also detected five K. variicola isolates within the K. pneumoniae/K. variicola group [1]
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|---|---|
| Cell Assay |
To perform the D-(+)-Melezitose (hydrate) assimilation assay, clinical Klebsiella isolates were first purified and then inoculated into a culture medium where D-(+)-Melezitose (hydrate) served as the sole carbon source. The inoculated medium was incubated under standardized conditions for a set period to facilitate bacterial metabolic activity. The assimilation capacity was determined by observing the degree of bacterial growth: isolates that could metabolize D-(+)-Melezitose (hydrate) showed obvious proliferation, while those unable to assimilate it exhibited negligible or no growth. This assay was conducted in parallel with 16 conventional biochemical tests and two other supplementary tests (growth at 10°C and histamine assimilation) to comprehensively improve the accuracy of Klebsiella species typing [1]
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| References | |
| Additional Infomation |
This study did not study D-(+)-melatonin (hydrate) as a therapeutic agent, but rather used it as a biochemical tool for phenotypic identification of Klebsiella spp. This study aims to address the complexity of Klebsiella spp. classification caused by the limitations of traditional biochemical classification methods, and to identify the assimilation effect of D-(+)-melatonin (hydrate) as one of the three necessary supplementary detection methods for reliable Klebsiella spp. isolate typing. PCR typing of common sequences among repetitive sequences in Enterobacteriaceae further revealed the genotypic diversity among isolates, which is consistent with the phenotypic classification results including D-(+)-melatonin (hydrate) assimilation data [1].
|
| Molecular Formula |
C18H34O17
|
|---|---|
| Molecular Weight |
522.4524
|
| Exact Mass |
504.169
|
| CAS # |
207511-10-2
|
| PubChem CID |
16217716
|
| Appearance |
White to off-white solid powder
|
| Density |
1.8±0.1 g/cm3
|
| Boiling Point |
881.8±65.0 °C at 760 mmHg
|
| Melting Point |
160 °C (dec.)(lit.)
|
| Flash Point |
487.1±34.3 °C
|
| Vapour Pressure |
0.0±0.6 mmHg at 25°C
|
| Index of Refraction |
1.676
|
| LogP |
-5.79
|
| Hydrogen Bond Donor Count |
12
|
| Hydrogen Bond Acceptor Count |
17
|
| Rotatable Bond Count |
8
|
| Heavy Atom Count |
35
|
| Complexity |
655
|
| Defined Atom Stereocenter Count |
14
|
| SMILES |
C([C@@H]1[C@H]([C@@H]([C@H]([C@H](O1)O[C@H]2[C@@H]([C@H](O[C@@]2(CO)O[C@@H]3[C@@H]([C@H]([C@@H]([C@H](O3)CO)O)O)O)CO)O)O)O)O)O.O
|
| InChi Key |
CFXAVQUXSYFPDE-ZDVKUAOASA-N
|
| InChi Code |
InChI=1S/C18H32O16.H2O/c19-1-5-8(23)11(26)13(28)16(30-5)32-15-10(25)7(3-21)33-18(15,4-22)34-17-14(29)12(27)9(24)6(2-20)31-17;/h5-17,19-29H,1-4H2;1H2/t5-,6-,7-,8-,9-,10-,11+,12+,13-,14-,15+,16-,17-,18+;/m1./s1
|
| Chemical Name |
(2R,3R,4S,5S,6R)-2-[(2S,3S,4R,5R)-4-hydroxy-2,5-bis(hydroxymethyl)-2-[(2R,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxolan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol;hydrate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O : ~100 mg/mL
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (Infinity mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.9141 mL | 9.5703 mL | 19.1406 mL | |
| 5 mM | 0.3828 mL | 1.9141 mL | 3.8281 mL | |
| 10 mM | 0.1914 mL | 0.9570 mL | 1.9141 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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