| Size | Price | Stock | Qty |
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| 100mg |
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| 500mg |
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| 1g |
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| Other Sizes |
| Targets |
D-Desthiobiotin targets avidin, streptavidin, and other biotin-binding proteins. Its strong affinity for avidin or streptavidin makes it useful in biotin-streptavidin-based assays. The compound binds to avidin and streptavidin with lower affinity compared to biotin, making it suitable for applications requiring temporary and reversible binding. D-Desthiobiotin also targets NCOR2 with a potency of 1000.0. As an endogenous metabolite, it participates in biotin-related metabolic pathways.
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| ln Vitro |
In vitro, D-Desthiobiotin is used in affinity chromatography and protein chromatography for protein purification and isolation. Its applications extend to in vitro and in vivo protein labeling, detection, and cellular separation techniques within biochemical research. The compound's reversible binding to streptavidin and avidin makes it valuable for applications requiring temporary and reversible binding. D-Desthiobiotin is a versatile biotin analog employed in protein and cell labeling, detection, and isolation. It is a precursor in the synthesis of biotin.
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| ln Vivo |
In vivo, D-Desthiobiotin is used for protein labeling, detection, and cellular separation techniques. Its applications extend to in vivo protein labeling and detection within biochemical research. The compound's reversible binding to streptavidin and avidin makes it suitable for in vivo applications where temporary binding is desired. D-Desthiobiotin is a biotin derivative used in various research applications. Further in vivo studies have evaluated its utility in protein labeling and detection systems.
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| Enzyme Assay |
D-Desthiobiotin binding assays involve measuring affinity for avidin, streptavidin, and other biotin-binding proteins. Binding affinity is assessed using surface plasmon resonance, isothermal titration calorimetry, or fluorescence-based binding assays. The compound's lower affinity compared to biotin enables reversible binding studies. For NCOR2 binding, affinity assays are performed using appropriate detection methods. Assays are performed in appropriate buffer systems with positive controls such as biotin. Competitive binding assays may be used to determine relative binding affinities.
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| Cell Assay |
D-Desthiobiotin cell-based assays are conducted in various cell types for protein labeling, detection, and cellular separation. Cells are cultured in appropriate media and treated with D-Desthiobiotin-conjugated probes. Labeling efficiency is assessed by fluorescence microscopy or flow cytometry. For affinity chromatography applications, cell lysates are passed through D-Desthiobiotin-conjugated affinity columns and bound proteins are eluted. Cell viability is assessed by standard assays. Experiments are performed with appropriate positive and negative controls.
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| Animal Protocol |
D-Desthiobiotin in vivo studies are conducted in animal models for protein labeling and detection. Animals are administered D-Desthiobiotin-conjugated probes via injection. Tissue distribution and protein labeling are assessed by imaging or biochemical analysis. The compound's reversible binding properties enable studies where temporary labeling is desired. For cellular separation studies, cells are labeled with D-Desthiobiotin and isolated using affinity methods. Animals are monitored for clinical signs. Tissues are collected for histopathological and biochemical analysis at study endpoints. Studies are conducted in accordance with institutional animal care guidelines.
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| ADME/Pharmacokinetics |
D-Desthiobiotin (MW 214.26 g/mol, C10H18N2O3) is a biotin derivative and protein cross-linking agent. It is a modified form of biotin lacking the thiophene ring. The compound is soluble in water and other appropriate solvents. It is stable under recommended storage conditions. D-Desthiobiotin is a precursor in the synthesis of biotin. Pharmacokinetic properties such as half-life, bioavailability, and tissue distribution would be determined in species-specific studies. The compound is typically used as a labeling reagent in research applications.
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| Toxicity/Toxicokinetics |
D-Desthiobiotin is generally well-tolerated as a research reagent. The compound is a biotin derivative with established safety profiles for in vitro and in vivo applications. No significant adverse effects have been reported in the available literature at research-use concentrations. The compound is intended for research use only. Standard laboratory safety practices should be employed when handling this compound. Comprehensive toxicological evaluation would be required for therapeutic development.
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| References | |
| Additional Infomation |
(4R,5S)-Dethiobiotin is the (4R,5S)-isomer of dethiobiotin. It is the conjugate acid of (4R,5S)-dethiobiotin(1-). It is the enantiomer of (4S,5R)-dethiobiotin. Dethiobiotin is a metabolite found or produced in Escherichia coli (K12 strain, MG1655 strain). Dethiobiotin has been reported to be detected in Scaphyglottis livida, and relevant data are available. Dethiobiotin is a metabolite found or produced in Saccharomyces cerevisiae. See also: dl-dethiobiotin (note moved to).
D-Desthiobiotin is a biotin derivative used in affinity chromatography and protein chromatography for protein purification and isolation. It binds to avidin and streptavidin with lower affinity than biotin, enabling reversible binding applications. The compound is used for protein and cell labeling, detection, and separation. D-Desthiobiotin targets NCOR2 with a potency of 1000.0. All applications are limited to non-human research use. |
| Molecular Formula |
C10H17N2O3-
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|---|---|
| Molecular Weight |
213.25358
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| Exact Mass |
214.131
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| CAS # |
533-48-2
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| Related CAS # |
D-Desthiobiotin; 533-48-2
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| PubChem CID |
445027
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| Appearance |
White to off-white solid powder
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| Density |
1.1±0.1 g/cm3
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| Boiling Point |
476.4±18.0 °C at 760 mmHg
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| Melting Point |
156-158 °C
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| Flash Point |
241.9±21.2 °C
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| Vapour Pressure |
0.0±2.6 mmHg at 25°C
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| Index of Refraction |
1.473
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| Source |
Endogenously produced metabolite: Human Gut Microbiota Metabolites
Originated from microorganisms: Synechococcus elongatus |
| LogP |
0.31
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
15
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| Complexity |
243
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| Defined Atom Stereocenter Count |
2
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| SMILES |
C[C@H]1[C@H](NC(N1)=O)CCCCCC(O)=O
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| InChi Key |
AUTOLBMXDDTRRT-JGVFFNPUSA-N
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| InChi Code |
InChI=1S/C10H18N2O3/c1-7-8(12-10(15)11-7)5-3-2-4-6-9(13)14/h7-8H,2-6H2,1H3,(H,13,14)(H2,11,12,15)/t7-,8+/m0/s1
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| Chemical Name |
6-[(4R,5S)-5-methyl-2-oxoimidazolidin-4-yl]hexanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~62.5 mg/mL (~291.70 mM)
H2O : ~1 mg/mL (~4.67 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (9.71 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (9.71 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (9.71 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 3.7 mg/mL (17.27 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication (<60°C). |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.6893 mL | 23.4467 mL | 46.8933 mL | |
| 5 mM | 0.9379 mL | 4.6893 mL | 9.3787 mL | |
| 10 mM | 0.4689 mL | 2.3447 mL | 4.6893 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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