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D-Cysteine

Alias: DCysteine; D Cysteine
Cat No.:V38868 Purity: ≥98%
D-Cysteine is the D-isomer of cysteine and a potent inhibitor of E. coli growth.
D-Cysteine
D-Cysteine Chemical Structure CAS No.: 921-01-7
Product category: New2
This product is for research use only, not for human use. We do not sell to patients.
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Product Description
D-Cysteine is the D-isomer of cysteine and a potent inhibitor of E. coli growth. D-Cysteine produces H2S mediated by D-amino acid (AA) oxidase and is a neuro-protective (neuro-protection) agent against cerebellar ataxia. D-Cysteine suppresses the growth and cariogenicity of dual-species biofilms formed by Streptococcus mutans and Streptococcus sanguineus.
Biological Activity I Assay Protocols (From Reference)
References

[1]. Seki T. Availability of D-cysteine as a protectant for cerebellar neurons. Nihon Yakurigaku Zasshi. 2019;154(3):133-137.

[2]. Role of D-cysteine desulfhydrase in the adaptation of Escherichia coli to D-cysteine. J Biol Chem. 2001 Nov 2;276(44):40864-72.

[3]. Effect of D-cysteine on dual-species biofilms of Streptococcus mutans and Streptococcus sanguinis. Sci Rep. 2019 Apr 30;9(1):6689.

Additional Infomation
D-cysteine is an optically active form of cysteine having D-configuration. It is a cysteine and a D-alpha-amino acid. It is a conjugate base of a D-cysteinium. It is a conjugate acid of a D-cysteinate(1-). It is an enantiomer of a L-cysteine. It is a tautomer of a D-cysteine zwitterion.
A thiol-containing non-essential amino acid that is oxidized to form CYSTINE.
D-Cysteine is a metabolite found in or produced by Escherichia coli (strain K12, MG1655).
D-cysteine has been reported in Indigofera hirsuta, Gossypium hirsutum, and other organisms with data available.
See also: Cysteine (annotation moved to).
These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C₃H₇NO₂S
Molecular Weight
121.16
Exact Mass
121.019
CAS #
921-01-7
PubChem CID
92851
Appearance
White to off-white solid powder
Density
1.3±0.1 g/cm3
Boiling Point
293.9±35.0 °C at 760 mmHg
Melting Point
230ºC
Flash Point
131.5±25.9 °C
Vapour Pressure
0.0±1.3 mmHg at 25°C
Index of Refraction
1.550
LogP
0.23
Hydrogen Bond Donor Count
3
Hydrogen Bond Acceptor Count
4
Rotatable Bond Count
2
Heavy Atom Count
7
Complexity
75.3
Defined Atom Stereocenter Count
1
SMILES
C([C@H](C(=O)O)N)S
InChi Key
XUJNEKJLAYXESH-UWTATZPHSA-N
InChi Code
InChI=1S/C3H7NO2S/c4-2(1-7)3(5)6/h2,7H,1,4H2,(H,5,6)/t2-/m1/s1
Chemical Name
(2S)-2-amino-3-sulfanylpropanoic acid
Synonyms
DCysteine; D Cysteine
HS Tariff Code
2934.99.9001
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO : ~1 mg/mL (~8.25 mM)
H2O : ~1 mg/mL (~8.25 mM)
Solubility (In Vivo)
Solubility in Formulation 1: 7.69 mg/mL (63.47 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication (<60°C).

 (Please use freshly prepared in vivo formulations for optimal results.)
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 8.2535 mL 41.2677 mL 82.5355 mL
5 mM 1.6507 mL 8.2535 mL 16.5071 mL
10 mM 0.8254 mL 4.1268 mL 8.2535 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

Calculator

Molarity Calculator allows you to calculate the mass, volume, and/or concentration required for a solution, as detailed below:

  • Calculate the Mass of a compound required to prepare a solution of known volume and concentration
  • Calculate the Volume of solution required to dissolve a compound of known mass to a desired concentration
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An example of molarity calculation using the molarity calculator is shown below:
What is the mass of compound required to make a 10 mM stock solution in 5 ml of DMSO given that the molecular weight of the compound is 350.26 g/mol?
  • Enter 350.26 in the Molecular Weight (MW) box
  • Enter 10 in the Concentration box and choose the correct unit (mM)
  • Enter 5 in the Volume box and choose the correct unit (mL)
  • Click the “Calculate” button
  • The answer of 17.513 mg appears in the Mass box. In a similar way, you may calculate the volume and concentration.

Dilution Calculator allows you to calculate how to dilute a stock solution of known concentrations. For example, you may Enter C1, C2 & V2 to calculate V1, as detailed below:

What volume of a given 10 mM stock solution is required to make 25 ml of a 25 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=25 μM, V2=25 ml and V1 is the unknown:
  • Enter 10 into the Concentration (Start) box and choose the correct unit (mM)
  • Enter 25 into the Concentration (End) box and select the correct unit (mM)
  • Enter 25 into the Volume (End) box and choose the correct unit (mL)
  • Click the “Calculate” button
  • The answer of 62.5 μL (0.1 ml) appears in the Volume (Start) box
g/mol

Molecular Weight Calculator allows you to calculate the molar mass and elemental composition of a compound, as detailed below:

Note: Chemical formula is case sensitive: C12H18N3O4  c12h18n3o4
Instructions to calculate molar mass (molecular weight) of a chemical compound:
  • To calculate molar mass of a chemical compound, please enter the chemical/molecular formula and click the “Calculate’ button.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
  • Molecular mass (or molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
  • Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
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Reconstitution Calculator allows you to calculate the volume of solvent required to reconstitute your vial.

  • Enter the mass of the reagent and the desired reconstitution concentration as well as the correct units
  • Click the “Calculate” button
  • The answer appears in the Volume (to add to vial) box
In vivo Formulation Calculator (Clear solution)
Step 1: Enter information below (Recommended: An additional animal to make allowance for loss during the experiment)
Step 2: Enter in vivo formulation (This is only a calculator, not the exact formulation for a specific product. Please contact us first if there is no in vivo formulation in the solubility section.)
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Calculation results

Working concentration mg/mL;

Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
             (2) Be sure to add the solvent(s) in order.

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