| Size | Price | Stock | Qty |
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| 500mg |
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| 1g |
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| Other Sizes |
| Targets |
This compound does not have a specific pharmacological target in the context of drug development. It is classified as a color additive and diagnostic aid rather than a therapeutic agent. Its primary function is as a pH indicator and fluorescent probe for biological systems. The FDA has established usage restrictions for this compound, limiting its concentration to no more than 0.01% by weight in externally applied drugs. As a pyrene dye, it interacts with biological systems primarily through its fluorescent properties rather than through specific receptor binding.
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| ln Vitro |
Despite being in a competitive setting, Pyran can distinguish between distinct cations and Cu+ ions. Pyran can detect ions in environmental samples thanks to its micromolar detection limit and quick fluorescence response (t1/2=1.66 minutes) to Cu+ ions. Pyran and Cu+ have an observed complexation stoichiometry of 2:1[1]. The effectiveness of the pH-sensitive fluorescent indicator pyran as an intravital corneal and anterior chamber probe was investigated. Fluorophotometry can be used to measure the concentrations in the cornea and anterior chamber after topical application, which can take many hours to reach. The ratio of fluorescence intensities at two excitation wavelengths, I463/I404, is used to calculate pH. This ratio depends on pH but is unaffected by fluorophore concentration or other factors that could change intensity. Pyranosine in the cornea and anterior chamber of rabbit eyes showed readily quantifiable changes in fluorescence ratios in response to eyelid closure and contact lens wear, suggesting sensitivity to minute pH variations [2].
In vitro, D & C Green No. 8 functions as a fluorescent pH indicator and a chemical sensor for copper ions. It exhibits pH-sensitive fluorescence, with excitation and emission wavelengths of 450 nm and 510 nm, respectively. The compound is used to stain DNA and chromosomes for fluorescent microscopy and flow cytometry applications. It binds preferentially to DNA rich in adenine and thymine by attaching in the minor groove. Due to its fluorescent properties, it enables detection and quantification in various biochemical assays. |
| ln Vivo |
In vivo, D & C Green No. 8 is not used as a therapeutic agent and does not exhibit pharmacological activity. Its primary in vivo application is as a color additive in externally applied drugs and cosmetics. The FDA has established that it may be safely used in externally applied drugs at concentrations not exceeding 0.01% by weight. Toxicological properties have not been fully investigated, and it is not intended for systemic administration.
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| Enzyme Assay |
In vitro enzymatic or receptor binding assays are not applicable for this compound as it is not a drug targeting specific enzymes or receptors. However, its fluorescent properties can be characterized using spectrophotometric methods. The compound's ability to act as a pH indicator can be assessed by measuring fluorescence intensity across a range of pH values. Its copper ion sensing capability can be evaluated by monitoring fluorescence changes upon addition of copper ions. The compound's purity and identity can be confirmed using HPLC and NMR analysis.
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| Cell Assay |
In vitro cell-based assays for D & C Green No. 8 primarily involve its use as a fluorescent stain. Cells are incubated with the compound, and fluorescence microscopy or flow cytometry is used to visualize cellular structures. DAPI, a related compound, binds preferentially to DNA rich in adenine and thymine. The compound is cell-permeable and forms a fluorescent complex by attaching in the minor groove of A-T rich sequences. These properties make it useful for staining DNA and chromosomes in various cell-based applications.
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| Animal Protocol |
In vivo animal studies are not typically performed with D & C Green No. 8 as a therapeutic agent. Its use in animals is limited to its role as a color additive in externally applied products. The FDA has reviewed the safety of this compound and established usage restrictions based on toxicological data. The Environmental Working Group has reported some evidence of damage to the developing fetus and chronic aquatic toxicity. However, comprehensive animal study protocols for this compound are not well-documented.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties are not well-characterized for D & C Green No. 8 as it is not intended for systemic absorption. The compound is a water-soluble dye with a molecular weight of 524.39 g/mol. It is used as a pH indicator for biological systems. When used in externally applied products, it is expected to remain on the skin surface with minimal absorption. The compound is stable to light and heat, making it useful as a fluorescence probe and optical sensor.
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| Toxicity/Toxicokinetics |
The toxicological properties of D & C Green No. 8 have not been fully investigated. The Environmental Working Group has classified it as having LOW concern for cancer, allergies, and immunotoxicity, but HIGH concern for developmental and reproductive toxicity. It has been reported to show some evidence of damage to the developing fetus. The compound also exhibits evidence of chronic and acute aquatic toxicity. The FDA has restricted its use to externally applied drugs at concentrations not exceeding 0.01% by weight.
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| References |
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| Additional Infomation |
Pyranone is an organosodium salt used as a fluorescent dye. It contains a pyran(3-) group derived from the hydride of pyrene.
D & C Green No. 8 is a synthetic pyrene color additive with the FDA Unique Ingredient Identifier UNII I2W85YOX9L. It is also known by several synonyms including Pyranine, Solvent Green 7, and C.I. 59040. The compound has a molecular formula of C16H7O10S3·3Na. It is classified as an indicator, reagent, or diagnostic aid. The compound is used in externally applied drugs and cosmetics with FDA certification required. The Code of Federal Regulations limits the levels of subsidiary colors in this additive. |
| Molecular Formula |
C16H7NA3O10S3
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|---|---|
| Molecular Weight |
524.37
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| Exact Mass |
523.889
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| CAS # |
6358-69-6
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| PubChem CID |
61388
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| Appearance |
Light yellow to yellow solid powder
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| Density |
2.15
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| Boiling Point |
171-178 °C10 mm Hg(lit.)
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| Melting Point |
62-63.5 °C(lit.)
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| Flash Point |
250 °C
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| LogP |
4.244
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
10
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
32
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| Complexity |
932
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
KXXXUIKPSVVSAW-UHFFFAOYSA-K
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| InChi Code |
InChI=1S/C16H10O10S3.3Na/c17-11-5-12(27(18,19)20)8-3-4-10-14(29(24,25)26)6-13(28(21,22)23)9-2-1-7(11)15(8)16(9)10;;;/h1-6,17H,(H,18,19,20)(H,21,22,23)(H,24,25,26);;;/q;3*+1/p-3
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| Chemical Name |
trisodium;8-hydroxypyrene-1,3,6-trisulfonate
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| Synonyms |
Green No. 204; Green 204; D & C Green no. 8
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~20 mg/mL (~38.14 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: 25 mg/mL (47.67 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with heating and sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.9071 mL | 9.5353 mL | 19.0705 mL | |
| 5 mM | 0.3814 mL | 1.9071 mL | 3.8141 mL | |
| 10 mM | 0.1907 mL | 0.9535 mL | 1.9071 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
| NCT Number | Recruitment | interventions | Conditions | Sponsor/Collaborators | Start Date | Phases |
| NCT01000311 | Completed | Biological: MenACWY-CRM Biological: DTaP-IPV/Hib |
Meningococcal Disease | Novartis Vaccines | November 2009 | Phase 3 |
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