| Size | Price | Stock | Qty |
|---|---|---|---|
| 5g |
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| Other Sizes |
| Targets |
D(-)-2-Aminobutyric acid acts as a substrate for D-amino acid oxidase (DAO) [1]
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|---|---|
| ln Vitro |
D(-)-2-aminobutyric acid (D-α-aminobutyric acid) is D-amino acid oxidase's substrate [1].
Spectroscopic analysis (ultraviolet-visible spectroscopy) confirmed the formation of an initial-stage complex between D(-)-2-Aminobutyric acid and D-amino acid oxidase [1] The binding of D(-)-2-Aminobutyric acid to DAO induced characteristic changes in the absorption spectrum of the enzyme, particularly at specific wavelengths corresponding to the enzyme-substrate interaction [1] This initial binding step is a prerequisite for the subsequent catalytic oxidation of D(-)-2-Aminobutyric acid by DAO [1] |
| Enzyme Assay |
D-amino acid oxidase was purified and dissolved in an appropriate buffer to a specific concentration [1]
The reaction system was prepared by mixing the enzyme solution with D(-)-2-Aminobutyric acid (at a concentration sufficient to initiate complex formation) in the same buffer, maintained at a constant temperature [1] Ultraviolet-visible absorption spectra of the mixture were recorded over a wavelength range of 250–400 nm at specified time intervals after mixing [1] Spectra of the free enzyme and free D(-)-2-Aminobutyric acid (under the same conditions) were measured as controls to compare and identify spectral changes induced by complex formation [1] |
| References | |
| Additional Infomation |
D-α-aminobutyric acid (DABA) is the optically active form of α-aminobutyric acid with the D-configuration. It is an α-aminobutyric acid and also a D-α-amino acid. It is an enantiomer of L-α-aminobutyric acid. (R)-2-aminobutyric acid has been reported in Euglena gracilis, and relevant data are available. D(-)-2-aminobutyric acid is a natural substrate of D-amino acid oxidase, which is involved in the metabolism of D-amino acids [1]. The initial complex formation between D(-)-2-aminobutyric acid and DAO is a non-covalent interaction that precedes the enzymatic oxidation reaction [1].
|
| Molecular Formula |
C4H9NO2
|
|---|---|
| Molecular Weight |
103.11976
|
| Exact Mass |
103.063
|
| CAS # |
2623-91-8
|
| Related CAS # |
D(-)-2-Aminobutyric acid-d6;1276197-57-9
|
| PubChem CID |
439691
|
| Appearance |
White to off-white solid powder
|
| Density |
1.1±0.1 g/cm3
|
| Boiling Point |
215.2±23.0 °C at 760 mmHg
|
| Melting Point |
300ºC
|
| Flash Point |
83.9±22.6 °C
|
| Vapour Pressure |
0.1±0.9 mmHg at 25°C
|
| Index of Refraction |
1.462
|
| LogP |
-0.15
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
7
|
| Complexity |
72.1
|
| Defined Atom Stereocenter Count |
1
|
| SMILES |
CC[C@H](C(=O)O)N
|
| InChi Key |
QWCKQJZIFLGMSD-GSVOUGTGSA-N
|
| InChi Code |
InChI=1S/C4H9NO2/c1-2-3(5)4(6)7/h3H,2,5H2,1H3,(H,6,7)/t3-/m1/s1
|
| Chemical Name |
(2R)-2-aminobutanoic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O : ~100 mg/mL (~969.74 mM)
DMSO :< 1 mg/mL |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 50 mg/mL (484.87 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 9.6974 mL | 48.4872 mL | 96.9744 mL | |
| 5 mM | 1.9395 mL | 9.6974 mL | 19.3949 mL | |
| 10 mM | 0.9697 mL | 4.8487 mL | 9.6974 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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